Gene:
Gene Symbol:
LPHN1
Gene Name:
latrophilin 1
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
Genentech clones
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00945746
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4422nts
Open reading frame: 1 to 4422
ATGGCACGCCTGGCCGCCGTGCTGTGGAACCTGTGCGTGACCGCCGTGCTGGTGACCAGC
GCCACTCAGGGACTGAGTCGGGCAGGACTGCCCTTCGGCCTGATGCGTAGAGAGCTGGCC
TGCGAGGGCTATCCAATCGAACTGCGCTGTCCCGGGAGCGACGTGATTATGGTGGAGAAC
GCTAATTACGGCCGGACCGACGATAAAATCTGCGATGCAGACCCTTTCCAGATGGAGAAT
GTGCAGTGTTACCTGCCAGACGCCTTTAAGATTATGTCCCAGCGATGCAACAATCGTACA
CAGTGCGTGGTCGTTGCCGGATCTGATGCTTTCCCTGACCCATGCCCGGGCACCTATAAG
TACCTGGAAGTGCAGTATGATTGCGTGCCCTACAAGGTGGAGCAGAAAGTGTTTGTCTGT
CCTGGCACCCTGCAGAAAGTGCTGGAGCCAACCTCAACACACGAAAGCGAGCATCAGTCC
GGTGCATGGTGTAAGGACCCCCTGCAGGCTGGGGACCGAATCTACGTGATGCCCTGGATT
CCCTATCGTACTGATACCCTGACAGAATACGCCTCCTGGGAGGACTATGTGGCAGCCAGA
CACACCACAACTTATCGGCTGCCCAACAGAGTGGATGGCACCGGGTTCGTGGTCTACGAC
GGAGCCGTGTTTTATAATAAGGAGCGGACCAGAAACATCGTCAAATACGACCTGAGGACA
CGCATCAAGTCAGGCGAAACTGTGATTAATACCGCCAACTACCATGATACAAGCCCTTAT
AGATGGGGCGGGAAAACTGACATCGACCTGGCCGTGGACGAAAATGGCCTGTGGGTCATC
TACGCTACCGAGGGAAACAATGGCCGGCTGGTGGTGAGCCAGCTGAACCCATATACACTG
AGATTCGAAGGCACCTGGGAGACAGGGTACGATAAGAGGAGTGCCTCAAATGCTTTTATG
GTCTGCGGCGTGCTGTACGTGCTGCGGAGCGTGTACGTGGACGATGACAGCGAGGCTGCA
GGGAACCGGGTGGACTACGCCTTCAACACAAATGCTAACAGAGAGGAACCTGTGAGCCTG
ACTTTCCCCAATCCTTACCAGTTTATCAGCTCCGTGGATTATAATCCAAGAGACAACCAG
CTGTATGTCTGGAACAATTACTTCGTGGTGCGGTATTCCCTGGAGTTTGGTCCACCTGAT
CCATCTGCAGGTCCAGCCACTTCTCCACCGCTGAGTACCACAACTACCGCTAGGCCAACC
CCGCTGACTTCCACCGCATCTCCAGCAGCTACAACTCCACTCAGGCGCGCCCCTCTGACC
ACACACCCAGTGGGAGCTATCAACCAGCTGGGTCCCGACCTGCCACCTGCAACTGCACCC
GTGCCTTCTACCAGACGGCCCCCCGCACCTAACCTGCATGTGAGTCCAGAGCTGTTCTGC
GAACCCCGCGAGGTCCGGAGAGTTCAGTGGCCTGCTACACAGCAGGGGATGCTGGTGGAA
CGGCCATGCCCGAAGGGCACTAGAGGGATCGCCAGCTTTCAGTGTCTGCCCGCCCTGGGC
CTGTGGAACCCCAGAGGCCCTGATCTGTCAAATTGTACCAGCCCCTGGGTGAACCAGGTC
GCCCAGAAGATCAAAAGTGGAGAAAATGCAGCCAACATTGCATCAGAGCTGGCCAGGCAC
ACACGCGGAAGCATCTACGCTGGCGACGTGAGCAGCAGCGTTAAGCTGATGGAGCAGCTG
CTGGACATCCTGGACGCTCAGCTGCAGGCCCTGCGGCCCATTGAACGTGAGAGCGCCGGC
AAGAATTATAACAAGATGCACAAACGAGAACGTACCTGCAAGGATTACATCAAGGCCGTG
GTCGAGACAGTGGACAATCTGCTGCGGCCCGAAGCTCTGGAGAGCTGGAAAGACATGAAC
GCAACCGAGCAGGTGCATACAGCCACTATGCTGCTGGACGTGCTGGAGGAAGGGGCCTTC
CTGCTGGCCGACAACGTGCGAGAACCTGCCCGGTTCCTGGCTGCAAAGGAAAACGTGGTG
CTGGAGGTCACCGTGCTGAACACTGAAGGACAGGTGCAGGAGCTGGTCTTCCCCCAGGAG
GAATATCCTAGAAAAAACTCCATCCAGCTGTCTGCCAAGACCATCAAGCAGAATAGTAGG
AACGGCGTCGTTAAGGTGGTCTTCATCCTGTACAACAACCTGGGCCTGTTCCTGAGCACC
GAAAATGCCACAGTGAAGCTGGCTGGAGAGGCAGGTCCAGGAGGTCCAGGTGGTGCTAGT
CTGGTTGTGAACAGCCAGGTCATCGCCGCTTCCATTAACAAAGAGAGCAGCCGGGTGTTC
CTGATGGACCCCGTGATCTTCACCGTGGCACACCTGGAAGACAAGAATCATTTCAATGCC
AACTGCTCTTTTTGGAACTACAGTGAGCGGAGCATGCTGGGATATTGGTCCACACAGGGT
TGTCGGCTGGTGGAATCTAATAAGACTCACACTACCTGCGCCTGTTCTCACCTGACCAAC
TTCGCAGTGCTGATGGCCCACAGGGAAATCTACCAGGGGCGCATTAACGAGCTGCTCCTG
AGTGTGATCACTTGGGTTGGAATCGTGATTAGTCTGGTCTGCCTCGCCATCTGTATTTCA
ACCTTCTGCTTTCTGCGAGGCCTGCAGACTGATCGTAATACCATCCATAAAAACCTGTGC
ATTAACCTGTTCCTGGCCGAGCTGCTGTTTCTGGTGGGAATTGACAAGACCCAGTATGAG
ATCGCATGCCCAATTTTCGCCGGCCTGCTGCACTACTTCTTTCTGGCAGCCTTTTCCTGG
CTGTGCCTGGAGGGGGTGCATCTGTACCTGCTGCTCGTGGAGGTCTTCGAAAGCGAGTAT
TCCAGGACTAAATACTATTACCTGGGAGGTTACTGCTTTCCCGCCCTGGTCGTTGGAATC
GCTGCAGCCATTGATTATCGGAGCTACGGCACCGAGAAGGCTTGTTGGCTGCGCGTGGAC
AATTACTTCATCTGGAGCTTCATCGGCCCCGTGAGCTTCGTGATCGTGGTCAACCTGGTG
TTCCTGATGGTGACCCTGCACAAAATGATTCGGAGCAGCAGCGTGCTGAAGCCAGATAGC
TCCCGCCTGGACAACATCAAATCCTGGGCACTGGGCGCTATTGCACTGCTGTTCCTGCTC
GGCCTGACATGGGCTTTCGGGCTGCTGTTCATCAATAAGGAGTCTGTGGTCATGGCATAC
CTGTTTACAACTTTCAACGCCTTTCAGGGGGTGTTCATTTTTGTCTTCCACTGCGCCCTG
CAGAAGAAAGTGCATAAGGAATACTCCAAATGTCTGAGGCACTCTTATTGCTGTATCCGC
AGTCCCCCTGGTGGGACACATGGGAGTCTGAAAACTTCAGCCATGCGGAGCAATACCAGA
TATTACACCGGAACACAGTCCCGCATCAGGCGCATGTGGAACGATACAGTGCGAAAGCAG
ACTGAGTCTAGTTTTATGGCCGGCGACATTAATTCTACTCCAACCCTGAACCGCGGCACT
ATGGGGAATCACCTGCTCACCAACCCTGTGCTGCAGCCACGGGGAGGCACCAGCCCATAT
AACACACTGATCGCCGAGTCCGTGGGATTCAATCCCTCAAGCCCACCCGTGTTCAACTCT
CCTGGCAGTTACAGGGAACCAAAGCATCCACTGGGCGGCAGAGAGGCCTGCGGCATGGAT
ACCCTGCCCCTGAACGGGAACTTCAACAATAGCTACTCCCTGAGAAGCGGCGATTTTCCC
CCTGGGGACGGAGGTCCTGAGCCACCCCGGGGCAGAAACCTGGCAGACGCTGCAGCCTTC
GAAAAAATGATCATTAGCGAGCTGGTGCACAACAATCTGAGGGGCAGCAGCAGCGCCGCA
AAGGGTCCACCTCCACCAGAGCCACCTGTGCCACCCGTGCCCGGTGGTGGAGGCGAGGAA
GAGGCCGGCGGGCCTGGAGGTGCCGACAGAGCTGAAATCGAGCTGCTCTATAAGGCTCTG
GAAGAGCCCCTGCTCCTGCCTAGGGCACAGAGCGTGCTGTACCAGTCCGATCTGGACGAA
TCTGAGAGTTGTACCGCTGAGGATGGCGCAACAAGCCGGCCTCTGTCAAGCCCCCCTGGA
AGAGACTCCCTCTATGCATCTGGTGCCAACCTGCGGGATTCACCTAGCTACCCAGACTCC
TCTCCCGAAGGCCCTAGCGAGGCTCTGCCACCACCACCTCCAGCACCACCAGGTCCTCCA
GAAATCTACTACACCAGCCGGCCCCCCGCCCTGGTGGCACGTAATCCCCTGCAGGGCTAT
TACCAGGTGCGGCGGCCCAGCCACGAAGGCTACCTGGCAGCTCCAGGCCTGGAGGGACCA
GGTCCTGATGGCGACGGGCAGATGCAGCTGGTGACCAGCCTG
Protein sequence predicted based on nucleotide sequence
MARLAAVLWNLCVTAVLVTSATQGLSRAGLPFGLMRRELACEGYPIELRCPGSDVIMVEN ANYGRTDDKICDADPFQMENVQCYLPDAFKIMSQRCNNRTQCVVVAGSDAFPDPCPGTYK YLEVQYDCVPYKVEQKVFVCPGTLQKVLEPTSTHESEHQSGAWCKDPLQAGDRIYVMPWI PYRTDTLTEYASWEDYVAARHTTTYRLPNRVDGTGFVVYDGAVFYNKERTRNIVKYDLRT RIKSGETVINTANYHDTSPYRWGGKTDIDLAVDENGLWVIYATEGNNGRLVVSQLNPYTL RFEGTWETGYDKRSASNAFMVCGVLYVLRSVYVDDDSEAAGNRVDYAFNTNANREEPVSL TFPNPYQFISSVDYNPRDNQLYVWNNYFVVRYSLEFGPPDPSAGPATSPPLSTTTTARPT PLTSTASPAATTPLRRAPLTTHPVGAINQLGPDLPPATAPVPSTRRPPAPNLHVSPELFC EPREVRRVQWPATQQGMLVERPCPKGTRGIASFQCLPALGLWNPRGPDLSNCTSPWVNQV AQKIKSGENAANIASELARHTRGSIYAGDVSSSVKLMEQLLDILDAQLQALRPIERESAG KNYNKMHKRERTCKDYIKAVVETVDNLLRPEALESWKDMNATEQVHTATMLLDVLEEGAF LLADNVREPARFLAAKENVVLEVTVLNTEGQVQELVFPQEEYPRKNSIQLSAKTIKQNSR NGVVKVVFILYNNLGLFLSTENATVKLAGEAGPGGPGGASLVVNSQVIAASINKESSRVF LMDPVIFTVAHLEDKNHFNANCSFWNYSERSMLGYWSTQGCRLVESNKTHTTCACSHLTN FAVLMAHREIYQGRINELLLSVITWVGIVISLVCLAICISTFCFLRGLQTDRNTIHKNLC INLFLAELLFLVGIDKTQYEIACPIFAGLLHYFFLAAFSWLCLEGVHLYLLLVEVFESEY SRTKYYYLGGYCFPALVVGIAAAIDYRSYGTEKACWLRVDNYFIWSFIGPVSFVIVVNLV FLMVTLHKMIRSSSVLKPDSSRLDNIKSWALGAIALLFLLGLTWAFGLLFINKESVVMAY LFTTFNAFQGVFIFVFHCALQKKVHKEYSKCLRHSYCCIRSPPGGTHGSLKTSAMRSNTR YYTGTQSRIRRMWNDTVRKQTESSFMAGDINSTPTLNRGTMGNHLLTNPVLQPRGGTSPY NTLIAESVGFNPSSPPVFNSPGSYREPKHPLGGREACGMDTLPLNGNFNNSYSLRSGDFP PGDGGPEPPRGRNLADAAAFEKMIISELVHNNLRGSSSAAKGPPPPEPPVPPVPGGGGEE EAGGPGGADRAEIELLYKALEEPLLLPRAQSVLYQSDLDESESCTAEDGATSRPLSSPPG RDSLYASGANLRDSPSYPDSSPEGPSEALPPPPPAPPGPPEIYYTSRPPALVARNPLQGY YQVRRPSHEGYLAAPGLEGPGPDGDGQMQLVTSL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
