Gene:
Gene Symbol:
BAI3
Gene Name:
brain-specific angiogenesis inhibitor 3
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
Genentech clones
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00945747
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4566nts
Open reading frame: 1 to 4566
ATGAAGGCCGTCAGAAACCTGCTCATCTACATCTTCAGCACATATCTGCTGGTCATGTTT
GGCTTCAATGCCGCTCAGGACTTCTGGTGCAGTACTCTGGTTAAGGGCGTGATCTACGGG
TCTTATAGCGTGAGCGAGATGTTTCCAAAAAACTTCACCAATTGTACATGGACTCTGGAA
AACCCCGACCCCACAAAGTACTCAATCTATCTGAAGTTCAGCAAGAAGGACCTGAGCTGC
TCCAATTTCTCTCTGCTGGCCTACCAGTTTGACCACTTCAGTCATGAGAAGATCAAGGAC
CTGCTCCGCAAGAACCACAGCATTATGCAGCTGTGCAACTCCAAGAATGCCTTTGTCTTC
CTGCAGTATGATAAAAACTTCATCCAGATTCGGAGAGTGTTTCCCACCAATTTCCCTGGC
CTGCAGAAGAAAGGGGAGGAAGACCAGAAGAGCTTCTTTGAGTTTCTGGTGCTGAACAAG
GTGAGCCCTAGTCAGTTCGGCTGCCACGTGCTGTGCACATGGCTGGAGAGCTGCCTGAAG
TCCGAAAATGGCCGGACTGAGAGCTGTGGGATTATGTACACCAAATGCACATGTCCCCAG
CATCTGGGCGAGTGGGGGATCGACGATCAGTCCCTGATTCTGCTGAACAATGTGGTCCTG
CCCCTGAACGAACAGACCGAGGGCTGCCTCACACAGGAGCTGCAGACCACACAGGTGTGC
AATCTGACCCGCGAAGCCAAGCGACCCCCTAAAGAGGAATTTGGAATGATGGGCGACCAC
ACAATCAAGTCACAGAGGCCCCGCAGCGTTCATGAAAAAAGAGTGCCTCAGGAGCAGGCA
GACGCAGCCAAGTTCATGGCACAGACCGGCGAGTCTGGTGTCGAGGAATGGAGCCAGTGG
AGTACTTGCTCAGTTACCTGTGGTCAGGGGTCCCAGGTGCGAACCCGTACATGCGTCTCT
CCCTACGGAACACACTGTTCCGGTCCTCTGCGAGAGAGCCGGGTGTGCAACAATACCGCC
CTGTGCCCAGTGCACGGCGTGTGGGAGGAATGGAGCCCTTGGTCCCTGTGCTCTTTTACC
TGTGGACGCGGTCAGCGGACTAGAACCAGGAGCTGCACACCACCACAGTATGGCGGGCGG
CCATGTGAAGGTCCAGAGACACACCATAAGCCATGCAACATCGCCCTGTGCCCAGTCGAT
GGCCAGTGGCAGGAGTGGAGCAGCTGGAGTCAGTGCTCAGTGACTTGTTCTAATGGCACC
CAGCAGCGGAGCCGGCAGTGCACCGCTGCAGCCCACGGAGGCAGCGAGTGTCGTGGACCC
TGGGCCGAATCACGAGAGTGCTACAACCCCGAATGTACAGCTAACGGCCAGTGGAATCAG
TGGGGACATTGGAGCGGTTGCTCAAAGAGCTGTGACGGCGGGTGGGAGAGGCGCATCAGG
ACATGCCAGGGCGCCGTCATTACTGGGCAGCAGTGTGAAGGCACCGGCGAGGAGGTGCGG
CGGTGCAATGAGCAGCGGTGCCCAGCTCCTTACGAAATCTGCCCCGAGGATTATCTGATG
TCTATGGTGTGGAAACGGACCCCTGCAGGCGACCTGGCCTTCAACCAGTGCCCACTGAAT
GCCACAGGAACTACCAGCCGGAGATGTTCCCTGAGCCTGCACGGCGTGGCCTTTTGGGAG
CAGCCTAGTTTCGCTCGGTGCATTTCAAACGAATATAGACACCTGCAGCATAGCATCAAG
GAGCATCTGGCTAAAGGGCAGAGAATGCTGGCAGGAGACGGCATGTCCCAGGTGACAAAG
ACCCTGCTGGACCTGACCCAGAGGAAAAACTTTTACGCCGGGGACCTGCTCATGAGCGTT
GAGATTCTGAGGAATGTGACCGATACATTCAAGCGCGCCTCCTATATTCCTGCTTCTGAC
GGAGTGCAGAACTTCTTTCAGATCGTCTCTAACCTGCTGGACGAGGAAAATAAGGAAAAA
TGGGAGGATGCCCAGCAAATCTACCCAGGAAGCATTGAACTGATGCAGGTCATCGAGGAT
TTTATCCACATTGTTGGCATGGGGATGATGGACTTCCAGAACTCCTATCTGATGACTGGC
AACGTGGTGGCATCAATTCAGAAGCTGCCTGCTGCAAGCGTCCTGACCGACATCAACTTC
CCAATGAAGGGAAGGAAGGGTATGGTGGACTGGGCCCGGAACAGCGAGGATAGGGTGGTC
ATCCCAAAGTCCATTTTTACCCCCGTGAGCAGCAAAGAACTGGATGAGAGCAGCGTGTTC
GTGCTGGGGGCCGTGCTGTACAAGAACCTGGACCTGATTCTGCCTACACTGAGAAACTAT
ACTGTGATCAATAGCAAGATCATTGTGGTCACAATTAGGCCAGAGCCGAAAACAACTGAC
TCCTTTCTGGAAATCGAGCTGGCCCACCTGGCTAACGGCACCCTGAACCCATACTGCGTG
CTGTGGGACGATAGTAAGACCAATGAATCACTGGGAACTTGGAGCACCCAGGGTTGTAAG
ACAGTGCTGACTGACGCCTCACATACAAAATGCCTGTGCGATCGCCTGAGCACTTTCGCA
ATCCTGGCCCAGCAGCCCCGGGAAATCATTATGGAGTCCTCTGGCACACCTAGCGTGACC
CTGATTGTTGGAAGCGGCCTGTCCTGTCTGGCCCTGATCACACTGGCCGTGGTGTACGCC
GCTCTGTGGCGTTATATCCGGAGCGAGAGGAGTATCATTCTGATCAACTTCTGCCTGAGC
ATCATTAGTTCAAATATCCTCATTCTGGTGGGACAGACTCAGACCCACAACAAGTCCATC
TGCACCACAACTACCGCCTTCCTGCATTTCTTTTTCCTGGCCAGTTTCTGTTGGGTCCTG
ACCGAGGCTTGGCAGTCATACATGGCAGTGACCGGCAAGATCCGTACACGGCTGATTAGA
AAGCGGTTCCTGTGCCTGGGTTGGGGGCTGCCCGCCCTGGTCGTTGCAACTTCTGTGGGC
TTCACACGGACTAAGGGATACGGCACCGATCACTATTGCTGGCTGAGCCTGGAGGGAGGC
CTGCTCTATGCCTTTGTGGGTCCTGCAGCCGCTGTGGTCCTGGTGAACATGGTCATCGGC
ATCCTGGTGTTCAACAAGCTGGTGAGCCGGGACGGCATCCTGGACAAGAAACTGAAACAC
CGGGCAGGACAGATGTCTGAGCCACATAGTGGCCTGACCCTGAAGTGCGCCAAATGTGGC
GTGGTGAGCACCACTGCTCTGTCTGCAACCACAGCCAGTAACGCTATGGCATCACTGTGG
AGCAGCTGCGTGGTGCTGCCACTGCTGGCCCTGACCTGGATGTCCGCTGTGCTGGCAATG
ACAGATAAGCGGAGCATCCTCTTTCAGATTCTGTTTGCAGTGTTCGACTCTCTGCAGGGG
TTCGTTATCGTGATGGTCCACTGCATTCTGAGGCGTGAGGTGCAGGACGCTTTCCGCTGC
CGACTGCGTAACTGTCAGGACCCCATCAATGCTGACAGCAGCAGCAGCTTCCCTAACGGA
CACGCACAGATCATGACCGATTTCGAAAAGGACGTGGACATCGCCTGCAGGAGCGTGCTG
CATAAAGACATCGGCCCCTGTCGAGCAGCCACCATCACAGGCACCCTGAGCCGGATCAGC
CTGAACGACGATGAGGAAGAGAAGGGAACTAATCCAGAGGGCCTGAGTTACTCAACCCTG
CCGGGAAATGTGATCAGCAAAGTCATCATTCAGCAGCCCACCGGCCTGCACATGCCTATG
AGCATGAACGAGCTGTCCAATCCATGCCTGAAGAAAGAAAACTCCGAGCTGCGACGTACC
GTGTACCTGTGCACCGACGATAACCTGCGCGGCGCCGACATGGATATTGTGCATCCCCAG
GAAAGAATGATGGAGTCCGATTATATCGTCATGCCAAGGTCCTCTGTTAACAATCAGCCG
TCTATGAAGGAAGAGAGTAAAATGAACATCGGGATGGAAACCCTGCCACACGAGCGCCTG
CTCCATTACAAGGTTAATCCCGAGTTTAACATGAATCCCCCTGTGATGGACCAGTTCAAC
ATGAACCTGGAGCAGCACCTGGCTCCCCAGGAGCACATGCAGAACCTGCCATTTGAACCG
CGGACAGCCGTGAAAAACTTCATGGCCAGCGAACTGGACGATAATGCAGGCCTGAGCCGG
AGCGAGACAGGGTCCACAATCTCTATGAGTTCACTGGAACGGAGAAAGAGTAGGTATTCA
GACCTGGATTTTGAGAAAGTGATGCACACCCGGAAAAGACACATGGAGCTGTTCCAGGAG
CTGAACCAGAAATTTCAGACACTGGACAGGTTCCGCGATATTCCTAACACCAGCAGCATG
GAGAATCCCGCTCCTAACAAGAATCCCTGGGACACTTTTAAAAACCCCTCCGAATACCCT
CACTATACTACCATCAATGTGCTGGACACAGAAGCAAAGGATGCCCTGGAGCTGCGGCCC
GCAGAATGGGAGAAATGCCTCAATCTGCCCCTGGACGTGCAGGAAGGCGATTTCCAGACC
GAGGTC
Protein sequence predicted based on nucleotide sequence
MKAVRNLLIYIFSTYLLVMFGFNAAQDFWCSTLVKGVIYGSYSVSEMFPKNFTNCTWTLE NPDPTKYSIYLKFSKKDLSCSNFSLLAYQFDHFSHEKIKDLLRKNHSIMQLCNSKNAFVF LQYDKNFIQIRRVFPTNFPGLQKKGEEDQKSFFEFLVLNKVSPSQFGCHVLCTWLESCLK SENGRTESCGIMYTKCTCPQHLGEWGIDDQSLILLNNVVLPLNEQTEGCLTQELQTTQVC NLTREAKRPPKEEFGMMGDHTIKSQRPRSVHEKRVPQEQADAAKFMAQTGESGVEEWSQW STCSVTCGQGSQVRTRTCVSPYGTHCSGPLRESRVCNNTALCPVHGVWEEWSPWSLCSFT CGRGQRTRTRSCTPPQYGGRPCEGPETHHKPCNIALCPVDGQWQEWSSWSQCSVTCSNGT QQRSRQCTAAAHGGSECRGPWAESRECYNPECTANGQWNQWGHWSGCSKSCDGGWERRIR TCQGAVITGQQCEGTGEEVRRCNEQRCPAPYEICPEDYLMSMVWKRTPAGDLAFNQCPLN ATGTTSRRCSLSLHGVAFWEQPSFARCISNEYRHLQHSIKEHLAKGQRMLAGDGMSQVTK TLLDLTQRKNFYAGDLLMSVEILRNVTDTFKRASYIPASDGVQNFFQIVSNLLDEENKEK WEDAQQIYPGSIELMQVIEDFIHIVGMGMMDFQNSYLMTGNVVASIQKLPAASVLTDINF PMKGRKGMVDWARNSEDRVVIPKSIFTPVSSKELDESSVFVLGAVLYKNLDLILPTLRNY TVINSKIIVVTIRPEPKTTDSFLEIELAHLANGTLNPYCVLWDDSKTNESLGTWSTQGCK TVLTDASHTKCLCDRLSTFAILAQQPREIIMESSGTPSVTLIVGSGLSCLALITLAVVYA ALWRYIRSERSIILINFCLSIISSNILILVGQTQTHNKSICTTTTAFLHFFFLASFCWVL TEAWQSYMAVTGKIRTRLIRKRFLCLGWGLPALVVATSVGFTRTKGYGTDHYCWLSLEGG LLYAFVGPAAAVVLVNMVIGILVFNKLVSRDGILDKKLKHRAGQMSEPHSGLTLKCAKCG VVSTTALSATTASNAMASLWSSCVVLPLLALTWMSAVLAMTDKRSILFQILFAVFDSLQG FVIVMVHCILRREVQDAFRCRLRNCQDPINADSSSSFPNGHAQIMTDFEKDVDIACRSVL HKDIGPCRAATITGTLSRISLNDDEEEKGTNPEGLSYSTLPGNVISKVIIQQPTGLHMPM SMNELSNPCLKKENSELRRTVYLCTDDNLRGADMDIVHPQERMMESDYIVMPRSSVNNQP SMKEESKMNIGMETLPHERLLHYKVNPEFNMNPPVMDQFNMNLEQHLAPQEHMQNLPFEP RTAVKNFMASELDDNAGLSRSETGSTISMSSLERRKSRYSDLDFEKVMHTRKRHMELFQE LNQKFQTLDRFRDIPNTSSMENPAPNKNPWDTFKNPSEYPHYTTINVLDTEAKDALELRP AEWEKCLNLPLDVQEGDFQTEV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
