Gene:
Gene Symbol:
BAI1
Gene Name:
brain-specific angiogenesis inhibitor 1
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
Genentech clones
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00945748
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4752nts
Open reading frame: 1 to 4752
ATGAGGGGTCAGGCCGCTGCACCTGGTCCCGTGTGGATTCTGGCACCACTGCTCCTGCTG
CTGCTGCTGCTCGGCCGGCGGGCCAGAGCCGCCGCCGGAGCCGACGCTGGACCAGGTCCA
GAGCCTTGCGCCACACTGGTGCAGGGCAAGTTCTTTGGGTACTTTAGCGCCGCTGCAGTC
TTCCCAGCAAACGCCTCACGGTGTAGCTGGACACTGAGAAATCCCGACCCCAGGCGCTAC
ACTCTGTATATGAAGGTGGCTAAAGCACCCGTCCCTTGCTCTGGTCCAGGTCGCGTGCGA
ACCTACCAGTTTGACAGTTTCCTGGAGTCAACACGGACTTATCTGGGCGTCGAGAGCTTC
GACGAGGTTCTGAGACTGTGCGATCCATCAGCACCCCTGGCCTTCCTGCAGGCCAGCAAG
CAGTTTCTGCAGATGCGGCGGCAGCAGCCACCTCAGCACGATGGCCTGCGGCCCCGGGCC
GGACCACCAGGTCCTACAGACGATTTCTCCGTGGAGTATCTGGTGGTCGGCAACCGTAAT
CCTTCTCGGGCCGCTTGCCAGATGCTGTGCAGGTGGCTCGACGCTTGCCTGGCAGGCAGC
CGGAGCAGCCATCCATGTGGCATCATGCAGACCCCATGCGCATGTCTGGGTGGTGAAGCT
GGAGGTCCTGCAGCAGGACCACTGGCCCCCCGGGGCGACGTGTGCCTGCGCGATGCCGTC
GCTGGCGGGCCAGAGAACTGTCTGACCAGCCTGACACAGGATAGGGGAGGCCACGGCGCT
ACCGGTGGTTGGAAGCTGTGGAGCCTGTGGGGCGAGTGCACACGCGACTGTGGAGGTGGC
CTGCAGACCAGAACAAGGACTTGCCTGCCAGCACCTGGTGTGGAAGGTGGAGGTTGTGAG
GGCGTCCTGGAGGAAGGGAGACAGTGCAATAGGGAGGCCTGCGGTCCAGCAGGTCGCACC
TCTAGTCGAAGTCAGTCACTGCGGAGCACCGATGCACGGAGAAGGGAGGAGCTGGGCGAC
GAGCTGCAGCAGTTTGGTTTCCCAGCTCCTCAGACTGGCGACCCCGCTGCAGAGGAATGG
AGCCCCTGGAGCGTGTGCAGCAGCACCTGTGGAGAGGGTTGGCAGACCCGCACACGATTC
TGCGTGTCCTCTAGTTACAGCACCCAGTGTTCCGGACCACTCCGGGAACAGAGACTGTGC
AACAATAGCGCCGTCTGTCCTGTGCACGGCGCTTGGGATGAGTGGAGCCCCTGGAGCCTG
TGCAGCAGCACCTGTGGCCGGGGCTTCCGGGACCGGACTAGAACCTGCAGACCCCCTCAG
TTTGGCGGGAACCCTTGTGAAGGCCCAGAGAAGCAGACCAAATTTTGCAATATCGCCCTG
TGCCCAGGAAGGGCAGTGGATGGTAACTGGAATGAATGGTCCTCTTGGAGCGCCTGCAGC
GCCAGCTGTTCTCAGGGGCGCCAGCAGAGGACACGCGAGTGCAACGGACCCTCCTACGGA
GGTGCCGAATGTCAGGGCCACTGGGTGGAGACTCGGGATTGCTTCCTGCAGCAGTGTCCT
GTGGACGGCAAGTGGCAGGCATGGGCCAGCTGGGGGAGTTGCTCAGTGACCTGTGGAGCA
GGCAGCCAGCGTCGAGAGAGAGTCTGCTCCGGTCCCTTCTTTGGTGGTGCAGCTTGTCAG
GGACCTCAGGACGAATATCGACAGTGCGGCACACAGCGGTGTCCAGAACCGCATGAGATT
TGCGACGAGGATAACTTTGGGGCTGTGATCTGGAAGGAAACACCAGCAGGAGAGGTGGCA
GCCGTCAGGTGCCCACGTAATGCAACTGGGCTGATTCTCCGGCGGTGCGAACTGGATGAG
GAAGGAATCGCTTACTGGGAGCCACCGACCTATATCCGCTGCGTGAGCATTGACTACCGG
AATATCCAGATGATGACAAGAGAACACCTGGCTAAGGCACAGAGGGGCCTGCCAGGTGAA
GGCGTGAGCGAAGTGATCCAGACCCTGGTGGAGATCAGCCAGGACGGCACAAGCTATTCC
GGGGACCTGCTGAGCACCATTGATGTGCTGCGCAACATGACCGAGATTTTCCGGAGGGCC
TACTATAGTCCCACACCTGGCGACGTGCAGAATTTCGTGCAGATTCTGTCAAACCTGCTG
GCCGAGGAAAATAGGGACAAGTGGGAGGAAGCACAGCTGGCCGGTCCAAACGCTAAAGAA
CTGTTTCGCCTCGTGGAGGACTTCGTGGACGTGATCGGCTTCCGGATGAAGGACCTGAGG
GATGCCTACCAGGTTACCGATAACCTGGTGCTGAGCATTCATAAACTGCCTGCTTCCGGC
GCAACCGACATCTCTTTCCCAATGAAAGGATGGAGGGCTACAGGCGACTGGGCAAAGGTG
CCTGAGGATCGCGTCACCGTGAGCAAAAGTGTCTTCTCTACTGGCCTGACCGAAGCCGAT
GAGGCCAGCGTGTTCGTTGTGGGCACCGTGCTGTACAGGAACCTGGGCAGCTTCCTGGCC
CTGCAGCGCAACACCACCGTGCTGAACAGCAAAGTGATCAGCGTGACTGTCAAGCCACCT
CCACGGAGCCTGCGTACCCCCCTGGAAATTGAGTTTGCCCACATGTATAACGGCACTACC
AATCAGACTTGTATCCTGTGGGACGAGACAGATGTGCCCAGTTCAAGCGCCCCACCACAG
CTGGGTCCTTGGTCTTGGCGTGGTTGCAGAACAGTGCCACTGGATGCTCTCCGAACTCGT
TGCCTGTGCGACCGGCTGTCCACATTCGCAATTCTGGCCCAGCTCTCTGCCGACGCTAAC
ATGGAAAAGGCCACCCTGCCTAGCGTCACACTGATCGTTGGATGTGGCGTGAGCAGCCTG
ACCCTGCTCATGCTGGTCATCATCTACGTGAGCGTGTGGAGGTATATCCGCAGCGAGCGA
TCCGTGATCCTGATCAACTTCTGCCTGAGCATCATTAGTTCAAATGCCCTGATCCTCATT
GGCCAGACACAGACTAGAAATAAGGTGGTGTGCACCCTCGTGGCTGCATTCCTGCACTTC
TTCTTCCTGAGCAGCTTCTGTTGGGTGCTGACAGAGGCTTGGCAGTCTTACATGGCAGTC
ACTGGCCACCTGCGGAACAGACTGATTAGGAAGCGGTTCCTGTGCCTCGGTTGGGGGCTG
CCCGCCCTGGTGGTCGCTATCAGCGTGGGATTCACAAAGGCCAAAGGTTACTCTACTATG
AATTATTGCTGGCTGAGCCTGGAGGGAGGCCTGCTCTATGCTTTTGTGGGTCCAGCCGCT
GCAGTGGTGCTGGTCAACATGGTCATCGGCATCCTGGTGTTCAACAAGCTGGTGAGCAAG
GACGGCATTACAGATAAGAAACTGAAGGAGAGGGCAGGGGCCAGCCTGTGGTCTAGTTGC
GTGGTGCTGCCCCTGCTGGCCCTGACTTGGATGTCAGCTGTGCTGGCAGTCACCGATCGG
CGGAGCGCCCTGTTTCAGATCCTGTTTGCCGTGTTCGACTCCCTGGAAGGCTTCGTCATT
GTTATGGTGCACTGCATCCTGCGTCGTGAGGTCCAGGACGCCGTTAAGTGTCGTGTGGTC
GATCGGCAGGAGGAAGGAAACGGCGACTCTGGCGGGAGTTTCCAGAATGGCCATGCACAG
CTGATGACCGATTTTGAGAAGGACGTGGACCTGGCATGCCGGAGCGTGCTGAACAAGGAC
ATTGCCGCTTGTCGGACCGCCACAATCACTGGCACCCTGAAGCGGCCCAGCCTGCCAGAG
GAAGAGAAGCTGAAGCTGGCCCACGCAAAGGGCCCTCCAACTAACTTTAATAGTCTGCCT
GCCAACGTGTCAAAACTGCACCTGCACGGCAGCCCCAGATACCCCGGAGGTCCACTGCCA
GATTTCCCAAATCATTCCCTGACCCTGAAGAGAGACAAAGCACCAAAGAGCAGCTTCGTG
GGCGACGGGGATATTTTTAAGAAGCTGGATTCAGAACTGAGCCGGGCACAGGAGAAGGCC
CTGGACACCAGCTACGTGATCCTGCCAACAGCCACTGCTACCCTGCGGCCCAAGCCTAAA
GAAGAGCCCAAATATTCCATCCACATTGACCAGATGCCTCAGACCAGACTGATCCACCTG
AGCACCGCCCCTGAGGCCAGCCTGCCAGCCCGGAGCCCCCCCTCTCGACAGCCTCCAAGC
GGTGGTCCACCAGAAGCACCTCCAGCACAGCCGCCCCCTCCACCTCCTCCTCCACCGCCC
CCTCCACAGCAGCCACTGCCACCACCTCCAAACCTGGAGCCTGCACCACCCTCCCTGGGA
GATCCAGGCGAGCCAGCAGCACACCCAGGACCCAGCACCGGTCCAAGCACTAAGAACGAA
AACGTGGCTACCCTGAGCGTGAGCAGCCTGGAGAGAAGGAAATCACGGTATGCAGAACTG
GACTTTGAGAAAATTATGCACACACGAAAGCGTCATCAGGACATGTTCCAGGACCTGAAC
AGAAAGCTGCAGCACGCTGCAGAAAAGGACAAAGAGGTGCTGGGGCCCGATTCTAAACCT
GAAAAGCAGCAGACCCCAAACAAGCGGCCCTGGGAGAGTCTGAGGAAGGCCCACGGCACC
CCAACTTGGGTGAAGAAAGAACTGGAGCCACTCCAGCCTTCTCCCCTGGAGCTGCGCAGT
GTGGAATGGGAGCGATCAGGTGCCACTATCCCCCTGGTGGGCCAGGACATCATTGATCTG
CAGACCGAGGTC
Protein sequence predicted based on nucleotide sequence
MRGQAAAPGPVWILAPLLLLLLLLGRRARAAAGADAGPGPEPCATLVQGKFFGYFSAAAV FPANASRCSWTLRNPDPRRYTLYMKVAKAPVPCSGPGRVRTYQFDSFLESTRTYLGVESF DEVLRLCDPSAPLAFLQASKQFLQMRRQQPPQHDGLRPRAGPPGPTDDFSVEYLVVGNRN PSRAACQMLCRWLDACLAGSRSSHPCGIMQTPCACLGGEAGGPAAGPLAPRGDVCLRDAV AGGPENCLTSLTQDRGGHGATGGWKLWSLWGECTRDCGGGLQTRTRTCLPAPGVEGGGCE GVLEEGRQCNREACGPAGRTSSRSQSLRSTDARRREELGDELQQFGFPAPQTGDPAAEEW SPWSVCSSTCGEGWQTRTRFCVSSSYSTQCSGPLREQRLCNNSAVCPVHGAWDEWSPWSL CSSTCGRGFRDRTRTCRPPQFGGNPCEGPEKQTKFCNIALCPGRAVDGNWNEWSSWSACS ASCSQGRQQRTRECNGPSYGGAECQGHWVETRDCFLQQCPVDGKWQAWASWGSCSVTCGA GSQRRERVCSGPFFGGAACQGPQDEYRQCGTQRCPEPHEICDEDNFGAVIWKETPAGEVA AVRCPRNATGLILRRCELDEEGIAYWEPPTYIRCVSIDYRNIQMMTREHLAKAQRGLPGE GVSEVIQTLVEISQDGTSYSGDLLSTIDVLRNMTEIFRRAYYSPTPGDVQNFVQILSNLL AEENRDKWEEAQLAGPNAKELFRLVEDFVDVIGFRMKDLRDAYQVTDNLVLSIHKLPASG ATDISFPMKGWRATGDWAKVPEDRVTVSKSVFSTGLTEADEASVFVVGTVLYRNLGSFLA LQRNTTVLNSKVISVTVKPPPRSLRTPLEIEFAHMYNGTTNQTCILWDETDVPSSSAPPQ LGPWSWRGCRTVPLDALRTRCLCDRLSTFAILAQLSADANMEKATLPSVTLIVGCGVSSL TLLMLVIIYVSVWRYIRSERSVILINFCLSIISSNALILIGQTQTRNKVVCTLVAAFLHF FFLSSFCWVLTEAWQSYMAVTGHLRNRLIRKRFLCLGWGLPALVVAISVGFTKAKGYSTM NYCWLSLEGGLLYAFVGPAAAVVLVNMVIGILVFNKLVSKDGITDKKLKERAGASLWSSC VVLPLLALTWMSAVLAVTDRRSALFQILFAVFDSLEGFVIVMVHCILRREVQDAVKCRVV DRQEEGNGDSGGSFQNGHAQLMTDFEKDVDLACRSVLNKDIAACRTATITGTLKRPSLPE EEKLKLAHAKGPPTNFNSLPANVSKLHLHGSPRYPGGPLPDFPNHSLTLKRDKAPKSSFV GDGDIFKKLDSELSRAQEKALDTSYVILPTATATLRPKPKEEPKYSIHIDQMPQTRLIHL STAPEASLPARSPPSRQPPSGGPPEAPPAQPPPPPPPPPPPPQQPLPPPPNLEPAPPSLG DPGEPAAHPGPSTGPSTKNENVATLSVSSLERRKSRYAELDFEKIMHTRKRHQDMFQDLN RKLQHAAEKDKEVLGPDSKPEKQQTPNKRPWESLRKAHGTPTWVKKELEPLQPSPLELRS VEWERSGATIPLVGQDIIDLQTEV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
