Gene:
Gene Symbol:
BAI2
Gene Name:
brain-specific angiogenesis inhibitor 2
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
Genentech clones
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00945749
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4755nts
Open reading frame: 1 to 4755
ATGGAGAACACTGGCTGGATGGGGAAAGGACATAGAATGACCCCAGCATGCCCACTGCTC
CTGTCTGTGATCCTGAGCCTGAGGCTGGCAACAGCCTTTGACCCTGCACCAAGCGCCTGT
TCCGCTCTGGCATCTGGCGTGCTGTATGGTGCCTTCAGCCTGCAGGACCTGTTCCCCACC
ATTGCTTCTGGCTGCAGTTGGACCCTGGAGAACCCCGACCCCACAAAGTATTCCCTCTAC
CTGCGGTTTAATAGACAGGAACAGGTGTGCGCACATTTCGCCCCTAGACTCCTGCCACTG
GACCACTACCTGGTCAACTTCACATGTCTGAGGCCTTCCCCAGAGGAAGCCGTGGCTCAG
GCAGAATCTGAGGTCGGCAGACCAGAGGAAGAGGAAGCAGAAGCCGCTGCAGGCCTGGAG
CTGTGCTCCGGCAGCGGTCCCTTTACCTTCCTGCACTTTGATAAGAATTTCGTGCAGCTG
TGCCTGTCAGCCGAACCAAGCGAGGCTCCTCGTCTGCTGGCCCCAGCCGCTCTGGCCTTT
CGGTTCGTGGAGGTCCTCCTGATCAACAATAACAATAGCTCCCAGTTTACCTGCGGCGTG
CTGTGCAGGTGGTCCGAGGAATGCGGCCGGGCAGCAGGTAGAGCTTGTGGATTCGCACAG
CCAGGTTGCTCTTGTCCTGGCGAGGCAGGGGCTGGATCAACCACAACTACCAGCCCTGGT
CCACCTGCTGCACATACCCTGTCCAACGCTCTGGTGCCCGGTGGTCCAGCACCACCAGCA
GAGGCCGACCTGCACTCTGGATCTAGTAATGATCTGTTCACAACTGAAATGCGCTATGGC
GAGGAACCAGAGGAAGAGCCCAAGGTGAAAACACAGTGGCCTCGGAGCGCCGACGAGCCA
GGGCTGTACATGGCACAGACTGGAGATCCTGCCGCTGAAGAGTGGAGCCCCTGGAGCGTG
TGCTCCCTGACTTGTGGTCAGGGCCTGCAGGTGCGGACCCGGAGCTGCGTCTCAAGCCCC
TACGGCACCCTGTGCAGTGGACCACTCAGGGAGACACGCCCTTGCAACAATTCCGCCACT
TGTCCAGTGCATGGGGTCTGGGAAGAGTGGGGAAGTTGGTCACTGTGCAGCAGGTCCTGT
GGGCGCGGATCTAGGAGTCGCATGCGAACCTGCGTGCCACCTCAGCACGGAGGCAAGGCC
TGCGAAGGACCAGAGCTGCAGACAAAGCTGTGCTCTATGGCAGCCTGTCCCGTGGAAGGC
CAGTGGCTGGAGTGGGGACCCTGGGGTCCATGCTCAACAAGCTGTGCCAACGGCACTCAG
CAGCGGAGCCGGAAGTGCTCTGTGGCTGGTCCTGCTTGGGCAACTTGTACCGGAGCACTG
ACAGACACTAGAGAATGCTCAAACCTGGAGTGTCCCGCCACCGATAGCAAGTGGGGGCCT
TGGAATGCTTGGAGCCTGTGCAGTAAAACATGTGACACTGGATGGCAGCGGCGGTTCCGG
ATGTGCCAGGCAACCGGCACACAGGGGTATCCATGTGAAGGCACCGGCGAAGAGGTGAAG
CCCTGCAGCGAGAAAAGATGTCCTGCCTTCCACGAAATGTGCAGGGATGAGTATGTCATG
CTGATGACCTGGAAGAAAGCTGCAGCCGGGGAGATCATCTACAACAAGTGCCCACCGAAT
GCCTCCGGATCTGCTAGTAGGCGCTGTCTCCTGAGCGCACAGGGTGTGGCATACTGGGGC
CTGCCCTCTTTTGCCCGGTGCATCAGTCATGAATACAGATACCTGTACCTGAGCCTGAGG
GAGCACCTGGCCAAAGGGCAGCGCATGCTGGCTGGGGAAGGAATGTCCCAGGTGGTGCGG
AGCCTGCAGGAGCTGCTGGCCCGACGTACCTACTATTCAGGCGACCTGCTGTTTAGCGTG
GACATCCTGAGAAACGTGACTGACACCTTCAAGAGGGCCACATACGTCCCCTCCGCTGAC
GACGTGCAGCGGTTCTTCCAGGTGGTGAGCTTCATGGTGGATGCAGAAAATAAGGAGAAA
TGGGACGATGCCCAGCAGGTGTCACCTGGCAGCGTCCACCTGCTGCGTGTCGTTGAGGAC
TTTATCCACCTGGTGGGGGATGCCCTGAAGGCCTTCCAGTCCTCTCTGATTGTTACTGAC
AACCTGGTCATCAGCATTCAGCGTGAACCCGTGAGCGCCGTGAGCAGCGACATCACCTTC
CCTATGCGGGGACGGAGAGGCATGAAGGACTGGGTGCGACATAGTGAAGATCGGCTGTTC
CTGCCAAAGGAGGTCCTGAGCCTGAGCAGCCCTGGCAAACCAGCCACAAGTGGCGCTGCA
GGGTCACCAGGTAGAGGCAGGGGTCCAGGAACTGTGCCCCCTGGTCCAGGTCACTCCCAT
CAGCGGCTGCTGCCAGCCGACCCCGATGAGTCTAGTTATTTCGTGATCGGTGCTGTCCTG
TACAGAACCCTGGGCCTGATTCTGCCACCACCCAGGCCTCCACTGGCCGTGACCAGCAGA
GTGATGACAGTTACCGTGCGGCCCCCCACACAGCCTCCAGCTGAACCCCTGATCACTGTG
GAGCTGTCTTATATCATTAACGGCACCACAGACCCTCACTGCGCCAGTTGGGACTACTCA
AGAGCCGATGCTTCAAGCGGGGACTGGGATACCGAAAATTGTCAGACCCTGGAGACACAG
GCCGCTCATACCAGGTGCCAGTGTCAGCACCTGTCCACATTTGCCGTGCTGGCTCAGCCA
CCAAAGGACCTGACCCTGGAGCTGGCCGGCAGCCCCAGCGTCCCACTGGTCATCGGTTGC
GCCGTGAGCTGTATGGCACTCCTGACCCTGCTGGCCATCTACGCAGCCTTTTGGCGGTTC
ATCAAGTCCGAGCGATCTATCATTCTGCTGAACTTCTGCCTGAGCATTCTCGCCTCCAAT
ATCCTGATTCTCGTCGGGCAGAGCCGGGTGCTGAGTAAGGGAGTGTGCACCATGACAGCT
GCATTTCTGCACTTCTTCTTCCTGAGCAGCTTCTGTTGGGTGCTGACTGAGGCATGGCAG
AGCTATCTGGCCGTCATCGGCCGCATGCGAACCCGTCTGGTGCGGAAAAGATTTCTGTGC
CTCGGTTGGGGACTGCCCGCCCTGGTGGTCGCAGTCAGCGTTGGGTTCACTCGGACCAAG
GGTTATGGCACCAGTTCATACTGTTGGCTGAGCCTGGAGGGTGGCCTGCTGTACGCCTTT
GTGGGTCCCGCCGCTGTGATCGTCCTGGTTAACATGCTCATTGGGATCATTGTCTTCAAT
AAGCTGATGGCCCGCGACGGGATTAGTGATAAATCAAAGAAACAGAGAGCAGGCAGCGAG
AGGTGCCCCTGGGCCAGCCTGCTGCTCCCATGCTCTGCATGTGGTGCCGTGCCCTCTCCA
CTGCTGAGCAGCGCCAGCGCCCGTAACGCTATGGCATCACTGTGGTCTAGTTGCGTGGTG
CTGCCCCTGCTGGCCCTGACTTGGATGAGTGCTGTGCTGGCAATGACCGACCGGCGGAGC
GTGCTGTTTCAGGCCCTGTTTGCCGTGTTCAACAGCGCACAGGGCTTTGTCATCACCGCC
GTTCACTGCTTCCTGCGACGTGAGGTGCAGGACGTGGTTAAGTGCCAGATGGGGGTCTGT
AGAGCCGATGAAAGCGAGGACTCCCCCGATTCTTGTAAGAACGGACAGCTGCAGATCCTG
AGCGACTTTGAGAAAGACGTGGATCTGGCCTGCCAGACCGTCCTGTTCAAGGAAGTTAAC
ACATGTAATCCTAGCACAATTACTGGCACCCTGAGCCGGCTGAGCCTGGACGAAGATGAA
GAGCCTAAGAGCTGCCTGGTGGGACCAGAGGGCAGCCTGAGCTTCAGCCCTCTGCCAGGA
AACATCCTGGTCCCAATGGCAGCCAGCCCAGGGCTGGGAGAACCTCCACCACCACAGGAG
GCAAATCCCGTGTACATGTGCGGCGAGGGAGGCCTGCGTCAGCTGGACCTGACCTGGCTG
CGGCCCACAGAACCAGGCTCCGAGGGGGATTACATGGTGCTGCCACGGCGGACCCTGAGC
CTGCAGCCAGGTGGTGGAGGTGGTGGTGGAGAAGACGCACCTAGGGCCCGCCCAGAGGGA
ACTCCAAGGCGTGCTGCAAAAACTGTCGCCCATACCGAGGGCTATCCTAGCTTTCTGTCC
GTGGACCACAGCGGCCTGGGCCTGGGTCCTGCCTACGGCAGCCTGCAGAACCCATACGGC
ATGACTTTCCAGCCTCCACCGCCCACCCCATCAGCCCGTCAGGTGCCCGAACCAGGCGAG
CGGAGCCGGACAATGCCTCGTACTGTCCCAGGCAGCACCATGAAGATGGGCTCACTGGAA
CGAAAGAAACTGCGTTACAGCGACCTGGATTTCGAGAAAGTGATGCACACTCGGAAAAGA
CATAGCGAACTGTATCACGAGCTGAACCAGAAATTTCATACATTCGACCGGTACAGAAGC
CAGAGCACCGCCAAGCGAGAAAAGCGGTGGAGCGTGAGCAGCGGTGGTGCAGCTGAGAGA
AGCGTGTGCACAGATAAGCCTTCCCCAGGAGAAAGGCCCTCCCTGTCTCAGCACCGGCGG
CACCAGAGTTGGTCAACTTTTAAGAGCATGACCCTGGGCAGCCTGCCTCCAAAACCCCGG
GAGCGGCTGACCCTGCACAGGGCAGCCGCTTGGGAACCAACCGAGCCACCAGACGGCGAT
TTCCAGACAGAAGTG
Protein sequence predicted based on nucleotide sequence
MENTGWMGKGHRMTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPT IASGCSWTLENPDPTKYSLYLRFNRQEQVCAHFAPRLLPLDHYLVNFTCLRPSPEEAVAQ AESEVGRPEEEEAEAAAGLELCSGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAF RFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGSTTTTSPG PPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEP GLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSAT CPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEG QWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGP WNAWSLCSKTCDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVM LMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLR EHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSAD DVQRFFQVVSFMVDAENKEKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTD NLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATSGAA GSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSR VMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQ AAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRF IKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQ SYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAF VGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSP LLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITA VHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVN TCNPSTITGTLSRLSLDEDEEPKSCLVGPEGSLSFSPLPGNILVPMAASPGLGEPPPPQE ANPVYMCGEGGLRQLDLTWLRPTEPGSEGDYMVLPRRTLSLQPGGGGGGGEDAPRARPEG TPRRAAKTVAHTEGYPSFLSVDHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGE RSRTMPRTVPGSTMKMGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRS QSTAKREKRWSVSSGGAAERSVCTDKPSPGERPSLSQHRRHQSWSTFKSMTLGSLPPKPR ERLTLHRAAAWEPTEPPDGDFQTEV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
