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PEP1 (Saccharomyces cerevisiae) in pBY011 (yeast expression vector)

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Explanation of Terms


Gene: Gene Symbol:  PEP1
Gene Name:  vacuolar protein sorting receptor for carboxypeptidase Y and proteinase A; related to Sortilin
Sequence             
Original Clone ID: FLH260085.01X
Keyword: None
Species: Saccharomyces cerevisiae
Type: cDNA
Vector Name: pBY011               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
ScCD00096315

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 4740nts         Open reading frame : 1 to 4740

ATGATATTACTTCATTTTGTCTATTCTCTTTGGGCCTTACTTCTCATTCCTTTAACTAAT
GCCGAAGAATTCACCCCCAAAGTGACAAAGACTATCGCGCAAGATTCATTTGATATATTA
AGCTTTGATGATTCCAACACTTTAATTAGAAAACAAGACACCTCCGTTACTATAAGCTTT
GACGATGGTGAAACATGGGAAAAAGTTGAAGGCATTGAAGGCGAAATCACTTGGATATAC
ATCGACCCCTTCAATAGACATGACAGAGCCGTTGCAACGGCAATGAATGGGTCGTACCTT
TATATAACCAATGATCAAGGTAAGTCATGGGAGCGTATAACGCTACCTGACTCCGGAGAA
AGTATTTCACCTCGTGAATGCTATATAGAAACCCATCCCTTGAATAAGAACTATTTTCTT
GCAAAGTGCAACTATTGTGAGAAAACAGAAGTAAACAATGACAATGAAGAGAATTCGGGG
GACGAAGAGGGACAATTTGAAATATTTAATATTACACGTTGCACAGACAAGGTTTTTGCA
AGTAATGATGGTGGAAAATCCTTTTCTGAGATCAAGTCTTCTCTAGAAAGGAACGAAAAT
AGTCCTATCAGCATTTCTGATTGTGGCTTTGCCAAGACCAGCAAAGATTCTGACCTTGAA
AGTAGTGATACCTCAATAATCTGTCTTTTTCAAAATATGCAGCTTATTATGGATGAGTTT
AGTTCTCCTTACACCGAAAGTAAATTGGTCCTAACTACGGACTGGGGCAAATCACTAAAA
GAATTTGACCAATTTAAAGATAAGGTCGTCAATGGTTACAGGATATTGAAATCTCACATG
GTTGTCTTAACCCAGGGCGACAGATATAATGATATGTCTTCCATGGATGTGTGGGTATCA
AATGATCTGTCAAACTTTAAAATGGCTTACATGCCTACTCAGTTAAGGCATTCTATGCAA
GGAGAAATATATGAGGACGCTATGGGAAGAATTATCTTGCCCATGAGTAGGGAAAGAAGT
GATCAAGAGGAGGATAAGGGCATCGTGTCTGAAATTTTAATTTCCGACTCACAAGGGTTA
AAATTTTCCCCCATCCCATGGACCGCAAATGAGGTGTTTGGTTATATTAATTTTTATCAA
CCTACTTACTTGAAAGGAACGATGATTGCCTCACTTTACCCTCTATCTAGGCGTCGTAAC
CGTAAAGGAAAAGCCAAAGGAGTAAAGAGTAAGGGGGTAACCAAAATATCTGTTGATAAT
GGCCTCACATGGACAATGTTAAAAGTTGTTGATCCAGATAACGCAGACTCATTCGACTGT
GATATTACTGATTTTGAGAATTGTTCGCTTCAAAATATGTTTTACACACGGGAGGGTTCC
ACTCCAACCGCCGGAATTCTAATGACAACAGGTATTGTTGGCGATGGTAGTGTCTTCGAC
TGGGGAGATCAAAGAACCTTTATTTCTAGGGATGGTGGCTTAACATGGAAACTCGCCTTT
GATTTTCCTTGTTTATACGCTGTTGGTGATTACGGAAATGTTATTGTGGCTATACCGTAT
AATGCGGATGAAGACGACGATCCTCAATCCGAATTTTATTACTCTTTAGACCAAGGTAAA
ACTTGGACCGAATATCAGCTAGAAACTACTATCTACCCAAATGAAGTAATGAATACAACG
CCCGACGGATCTGGAGCTAAATTTATTCTAAATGGGTTTACTTTGGCGCATATGGATGGT
ACAACGAATTTCATCTATGCAATTGATTTTTCAACAGCCTTTAATGATAAGACATGCGAA
GAAAATGATTTCGAGGATTGGAATTTAGCTGAGGGGAAGTGTGTCAATGGAGTCAAGTAC
AAGATCAGAAGAAGAAAACAGGACGCTCAGTGCTTGGTGAAGAAAGTTTTTGAAGACTTA
CAATTATTTGAGACTGCTTGTGACAAGTGTACCGAGGCTGATTACGAATGCGCGTTTGAA
TTTGTTAGGGACGCGACCGGGAAATGCGTACCAGACTACAACCTAATCGTTCTCTCTGAC
GTATGTGATAAGACAAAGAAAAAAACTGTGCCTGTAAAACCATTGCAACTAGTTAAAGGT
GATAAATGTAAAAAACCAATGACAGTCAAATCAGTGGATATTTCGTGTGAGGGAGTTCCA
AAGAAGGGAACGAATGATAAAGAAATAGTGGTTACAGAAAACAAATTTGATTTCAAGATT
CAATTCTATCAATACTTTGACACAGTCACCGACGAATCCCTCCTCATGATCAATTCAAGA
GGAGAAGCTTATATATCTCATGATGGTGGACAAACAATAAAAAGGTTCGACAGTAATGGT
GAAACAATTATTGAAGTTGTGTTTAATCCATACTACAATTCTTCAGCTTATCTGTTTGGT
TCCAAAGGTAGCATTTTCTCTACCCATGATAGGGGATACTCTTTTATGACTGCTAAATTG
CCCGAGGCTAGGCAGTTAGGTATGCCATTAGACTTTAACGCTAAGGCACAGGATACATTT
ATCTATTATGGTGGTAAGAATTGTGAGTCAATCTTAAGTCCGGAATGTCATGCGGTAGCA
TACCTGACCAATGATGGGGGCGAAACGTTTACGGAAATGCTTGATAATGCAATTCATTGT
GAGTTTGCGGGCTCACTTTTCAAATATCCGTCAAATGAGGATATGGTTATGTGTCAAGTG
AAGGAAAAGTCTTCGCAGACAAGAAGCTTAGTTTCTTCTACTGATTTTTTCCAGGATGAT
AAAAATACCGTCTTTGAAAATATTATCGGCTACTTATCCACTGGTGGCTATATCATCGTT
GCTGTTCCTCATGAGAACAACGAATTGAGAGCATACGTAACTATCGATGGTACTGAGTTT
GCCGAGGCAAAATTCCCATATGATGAAGATGTTGGGAAGCAAGAGGCATTCACTATATTA
GAGTCTGAGAAAGGATCGATATTCTTACATTTAGCAACAAACTTAGTACCAGGACGCGAT
TTTGGCAATCTTTTGAAATCCAACTCAAATGGTACTTCTTTTGTCACGTTGGAGCATGCC
GTTAATAGAAACACATTCGGCTATGTTGACTTTGAAAAAATTCAAGGTCTCGAAGGCATT
ATTCTCACCAACATCGTTTCAAATAGTGACAAGGTCGCCGAGAATAAAGAAGACAAACAA
TTGAAGACGAAGATCACCTTTAATGAAGGTTCAGATTGGAACTTTTTGAAACCTCCGAAG
AGGGATTCAGAAGGAAAAAAGTTTTCTTGCAGCTCCAAATCACTGGATGAGTGTTCATTG
CACTTACATGGCTATACTGAACGTAAGGATATTAGAGATACATATTCTTCCGGTTCTGCA
TTAGGAATGATGTTCGGCGTTGGCAACGTTGGTCCTAACCTTTTACCATATAAAGAATGT
TCCACCTTCTTCACCACCGATGGTGGCGAAACGTGGGCTGAAGTTAAGAAGACTCCTCAC
CAATGGGAATACGGTGACCACGGTGGGATTTTAGTTTTAGTTCCTGAAAACTCAGAAACT
GATTCTATTTCCTATTCTACCGATTTTGGTAAAACATGGAAAGATTATAAATTCTGCGCT
GATAAGGTTTTAGTAAAGGATATAACCACTGTTCCCAGGGATTCTGCTTTGAGATTTTTG
CTGTTTGGAGAGGCAGCAGATATTGGAGGCAGCTCATTTAGAACGTACACAATTGATTTT
AGAAACATCTTCGAAAGACAATGTGATTTCGACATCACTGGTAAGGAAAGCGCAGATTAT
AAATACTCTCCTCTGGGTTCCAAAAGCAATTGCCTATTTGGTCACCAAACCGAGTTTTTA
CGTAAAACCGATGAAAATTGTTTTATTGGGAATATTCCACTTTCTGAATTTTCAAGAAAT
ATCAAAAACTGTTCTTGTACAAGACAAGATTTCGAGTGTGATTACAACTTTTACAAAGCT
AACGATGGTACTTGTAAATTAGTCAAAGGACTAAGCCCAGCAAATGCTGCAGACGTTTGT
AAAAAAGAGCCAGATTTAATCGAATATTTTGAATCGTCAGGCTACAGAAAGATCCCTCTA
TCAACCTGTGAGGGTGGCCTGAAATTGGATGCTCCCTCATCACCACATGCTTGCCCAGGA
AAAGAAAAAGAATTCAAGGAAAAGTACTCAGTAAGTGCCGGTCCCTTTGCATTTATTTTC
ATTTCAATTCTTTTAATAATTTTCTTTGCCGCATGGTTTGTATATGACAGAGGTATCAGA
AGAAATTGGGGATTTGCAAGGTTTGGAGAAATTAGGCTAGGTGACGATGGTTTAATAGAA
AACAATAATACTGACAGAGTTGTCAATAACATTGTGAAATCAGGATTTTACGTTTTCTCA
AATATCGGGTCTCTTTTACAGCACACAAAAACTAATATAGCGCATGCTATCTCCAAAATT
AGAGGAAGGTTTGGAAACAGAACAGGTCCAAGCTACTCATCCCTGATCCATGATCAATTT
TTGGATGAAGCAGATGACCTGCTTGCTGGCCACGATGAAGACGCCAATGACTTATCCAGT
TTCATGGATCAGGGTAGTAATTTTGAAATCGAAGAAGATGATGTTCCAACACTTGAAGAA
GAGCATACATCATATACAGATCAACCTACGACCACCGATGTTCCAGATACATTACCAGAA
GGAAATGAGGAAAACATCGACAGGCCTGATTCTACAGCGCCATCTAACGAAAACCAGTAG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 4740
Start on reference sequence 1
Mutations:
None
Discrepancy:
g4267t,G1423W
                                

5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  yeast    Marker:  URA
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pBY011

pBY011 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Yeast expression vector with ARS1, CEN4, Gal1-10 promoter; amp resistance (URA3 for yeast); recombinational cloning.
Comments: This is a bacteria-yeast shuttle vector useful for expressing cDNAs in yeast that includes an auxotrophic marker for selection in yeast.
Size (bp): 8121
Parent Vector: Name: YCplac33
Description: Parent vector is a CEN4, URA3, ampR, ColE1 vector from R.D. Geitz described in Gene 74(2) 5270534 (1988). The Gal1-10 promoter and the Gateway-type attR recombination cassette (reference frame A, see information at the Invitrogen website) were inserted into the parent to create pBY011.
Empty Vector: None
Properties: Gateway, acceptor (destination), inducible expression, recombinational cloning, with tag/fusion/marker, yeast expression, yeast shuttle
Author Name: Bhullar
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin 3651 4333
negative selection marker ccdB ccdB negative selection marker gene LOST in recombined (with insert) form 4915 5124
primer M13 reverse M13 reverse sequencing primer 4702 4722
primer M13 forward M13 forward sequencing primer 7183 7200
promoter Gal1 Gal1-10 yeast promoter 6934 7145
promoter Ura3 promoter URA3 promoter 2349 2378
recombination site attR2 attR recombination site 2, lost or altered in recombined (with insert) form 4749 4764
recombination site attR1 attR recombination site 1, lost or altered in recombined (with insert) form 6439 6455
selectable marker Ura3 URA3 1460 2263
selectable marker CmR chloramphenicol resistance gene LOST in recombined (with insert) form 5566 6222
selectable marker AmpR ampicillin resistance gene 2894 3553
yeast centromere CEN yeast centromeric sequence 255 7396
yeast origin ARS1 ARS1 yeast origin of replication 0 0

 

  • Gene
  • Protein
  • Clone
  • Organism

Gene

Target GenBank: CAA84836

 

Protein

SMART domain : VPS10 :

Clone

Cloning Information : 260085
HIP Master Clone ID : 125206
Original Clone ID : FLH260085.01X

Organism


Species Specific ID: YBL017C