DNASU Plasmid Repository • 480.965.5697 | Email

GEA2 (Saccharomyces cerevisiae) in pBY011 (yeast expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  GEA2
Gene Name:  Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs), involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; similar to but not functionally redundant with Gea1p
Sequence             
Original Clone ID: FLH262667.01X
Keyword: None
Species: Saccharomyces cerevisiae
Type: cDNA
Vector Name: pBY011               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
ScCD00096986

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 4380nts         Open reading frame : 1 to 4380

ATGAGTGATAGGGAATTCGTCACGGTAGATCCCGTCACTATCATAATCAAAGAATGCATT
AATTTATCGACAGCGATGCGGAAATACTCTAAATTTACCTCTCAATCTGGAGTGGCCGCT
TTGCTGGGGGGAGGAAGTGAAATATTTAGCAATCAAGATGACTACTTGGCTCACACATTC
AACAATTTGAATACCAACAAGCACAATGATCCATTTTTATCTGGATTCATTCAGTTAAGA
CTTATGTTGAATAAACTGAAAAATCTAGATAATATAGATTCACTAACCATATTGCAGCCA
TTTTTATTAATTGTGAGTACAAGTTCCATTTCTGGTTACATCACTTCCCTGGCCCTGGAC
TCTTTGCAGAAATTCTTTACCTTGAATATCATCAATGAATCATCGCAAAACTATATTGGT
GCACACAGGGCGACGGTAAATGCTCTAACACATTGTAGGTTTGAAGGATCTCAACAACTT
TCTGATGATTCAGTTCTTTTGAAAGTCGTGTTTTTACTGCGTTCAATCGTCGACTCACCT
TACGGAGATTTATTATCAAACTCTATCATATATGACGTATTGCAAACGATTCTTTCATTG
GCTTGTAATAACAGAAGGAGCGAAGTCCTTAGGAATGCTGCACAATCAACAATGATAGCC
GTTACCGTAAAGATTTTCTCAAAACTAAAGACTATTGAGCCTGTTAATGTGAATCAAATA
TACATCAATGATGAAAGTTACACAAATGATGTATTGAAGGCCGATACAATTGGCACAAAT
GTAGAATCCAAAGAAGAAGGAAGTCAAGAAGATCCCATCGGCATGAAAGTGAATAATGAG
GAAGCTATTAGCGAGGACGATGGCATTGAAGAAGAGCATATTCATTCAGAGAAGAGCACA
AATGGCGCCGAACAACTAGATATTGTGCAAAAAACAACAAGATCAAATTCCAGGATCCAA
GCGTATGCTGATGATAACTATGGATTGCCCGTGGTTAGGCAATATTTAAACTTATTACTA
TCATTGATTGCGCCAGAAAATGAATTAAAACATTCATACTCCACTAGAATATTTGGCCTA
GAGTTAATTCAAACGGCATTAGAAATTTCAGGTGATCGATTGCAGCTATACCCACGGCTT
TTTACACTGATATCAGATCCTATTTTCAAAAGCATTTTGTTTATCATACAGAACACTACA
AAATTATCACTACTTCAAGCTACATTGCAGCTATTTACTACTCTAGTTGTTATATTGGGC
AACAACTTACAATTACAGATCGAGCTCACTCTAACAAGAATATTTTCTATTCTTTTAGAT
GATGGTACCGCAAATAACTCGAGTTCTGAAAATAAGAACAAGCCATCAATAATAAAGGAA
CTTCTAATTGAGCAAATATCCATCTTATGGACTAGGTCGCCATCTTTTTTTACTTCTACT
TTTATCAATTTCGATTGTAATCTCGATAGGGCAGACGTTTCCATAAACTTTTTGAAGGCT
TTGACTAAATTGGCCTTACCAGAATCCGCCTTAACTACCACAGAAAGTGTACCACCCATT
TGCCTTGAGGGATTGGTCTCCCTAGTCGATGATATGTTCGATCACATGAAGGACATTGAC
AGAGAAGAATTTGGCAGGCAAAAGAATGAAATGGAAATCTTAAAAAAGAGGGACCGTAAA
ACAGAGTTTATTGAATGTACCAATGCATTCAATGAAAAGCCCAAAAAGGGTATTCCGATG
TTAATAGAAAAAGGTTTCATTGCTTCCGACTCCGATAAAGATATTGCGGAGTTTCTTTTC
AATAATAACAACCGTATGAATAAAAAAACAATCGGTTTGCTACTTTGCCATCCGGACAAA
GTAAGCTTGTTGAATGAATATATTCGTTTGTTTGATTTTTCAGGGTTAAGGGTCGATGAA
GCTATTAGAATTTTGTTGACGAAATTTAGGTTGCCTGGTGAATCGCAACAAATTGAAAGA
ATCATCGAAGCCTTCTCGTCTGCGTATTGTGAAAATCAAGATTACGATCCATCCAAAATC
AGTGACAACGCGGAGGATGACATTTCTACTGTTCAACCAGACGCTGATTCTGTTTTCATT
TTAAGTTATTCAATTATTATGTTGAACACTGACCTACATAACCCTCAAGTGAAGGAACAC
ATGTCATTTGAAGATTACTCTGGTAACTTAAAGGGATGCTGTAATCACAAAGACTTCCCA
TTCTGGTATTTGGATAGAATTTACTGTTCAATCAGAGATAAAGAAATTGTTATGCCTGAA
GAGCACCACGGCAACGAAAAGTGGTTTGAAGATGCTTGGAATAACTTGATATCTTCAACT
ACTGTTATAACTGAAATAAAAAAAGACACACAATCTGTCATGGATAAATTAACACCCTTG
GAGCTTTTGAACTTTGATAGAGCAATTTTTAAACAAGTTGGCCCAAGTATTGTCAGTACT
TTATTCAACATTTACGTAGTTGCATCTGATGACCATATATCTACCAGAATGATAACAAGT
TTGGACAAATGTTCCTATATTTCCGCATTTTTTGACTTCAAAGATCTCTTTAATGATATA
CTAAACTCCATTGCTAAGGGCACTACTTTGATTAATTCAAGCCATGACGATGAACTTTCA
ACTTTAGCTTTTGAATATGGCCCAATGCCACTGGTGCAAATTAAATTCGAAGACACTAAC
ACTGAGATCCCGGTTAGTACAGATGCTGTTAGATTTGGTAGATCATTTAAGGGTCAACTA
AATACAGTTGTTTTTTTCCGGATTATTCGCAGGAACAAAGATCCTAAAATTTTCTCCAAG
GAATTATGGTTAAACATTGTTAATATTATACTAACATTGTACGAAGACTTGATTTTGTCT
CCTGATATTTTCCCTGATTTACAAAAAAGACTGAAATTAAGCAACTTGCCTAAGCCATCT
CCTGAAATTTCTATTAACAAGAGCAAAGAAAGCAAAGGTCTCTTATCAACATTTGCTTCT
TATTTAAAAGGTGATGAAGAACCCACAGAAGAGGAAATCAAATCCTCAAAAAAAGCGATG
GAGTGCATAAAGTCGAGTAATATTGCCGCCTCTGTCTTTGGAAATGAATCAAATATAACA
GCGGATTTAATAAAAACTTTACTAGACTCCGCCAAAACTGAGAAAAACGCAGATAATTCC
AGGTATTTTGAAGCAGAACTTTTATTTATCATCGAATTGACTATTGCATTATTTCTATTT
TGCAAAGAGGAGAAAGAATTAGGAAAGTTCATACTTCAAAAAGTTTTCCAACTTTCTCAC
ACGAAAGGCCTCACGAAAAGGACTGTTCGTAGAATGCTAACATACAAAATTTTGTTAATT
TCGTTATGTGCGGATCAGACGGAGTACTTGTCCAAATTAATAAACGATGAGCTGTTAAAA
AAGGGGGATATTTTTACCCAAAAATTTTTTGCAACTAATCAAGGTAAGGAATTTTTGAAG
AGACTATTTTCATTGACCGAATCAGAGTTTTATAGAGGATTTTTACTAGGAAATGAGAAT
TTTTGGAAATTTTTAAGAAAAGTTACAGCAATGAAAGAGCAGAGCGAGAGCATTTTTGAA
TATTTAAATGAATCGATCAAGACAGACAGCAATATTTTGACAAATGAGAACTTCATGTGG
GTCCTAGGACTATTAGATGAAATTTCATCAATGGGTGCCGTTGGAAATCACTGGGAAATA
GAATACAAGAAATTGACAGAAAGTGGTCATAAAATTGATAAGGAGAATCCATACAAGAAA
TCGATCGAATTATCATTGAAATCCATTCAACTAACATCACACTTGCTGGAAGATAATAAC
GATCTGCGTAAAAACGAGATATTCGCTATTATTCAAGCTTTGGCACATCAATGCATCAAT
CCGTGTAAGCAGATAAGTGAATTTGCAGTGGTAACGCTAGAGCAGACGCTCATCAATAAA
ATCGAAATTCCAACTAATGAGATGGAATCGGTAGAAGAATTAATTGAGGGCGGATTACTA
CCGTTGCTAAATTCGAGTGAAACACAGGAAGACCAGAAAATCCTCATTTCATCCATATTA
ACAATAATTTCAAATGTTTATTTGCATTATTTGAAACTAGGGAAGACAAGCAACGAAACG
TTTTTGAAAATTTTGAGTATTTTCAATAAATTTGTAGAGGACTCAGATATTGAAAAAAAG
CTACAGCAATTAATACTTGATAAGAAGAGTATTGAGAAGGGCAACGGTTCATCATCTCAT
GGATCTGCACATGAACAAACACCAGAGTCAAACGACGTTGAAATTGAGGCTACTGCGCCA
ATTGATGACAATACAGACGATGATAACAAACCGAAGTTATCTGATGTAGAAAAGGATTAG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 4380
Start on reference sequence 1
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  yeast    Marker:  URA
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pBY011

pBY011 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Yeast expression vector with ARS1, CEN4, Gal1-10 promoter; amp resistance (URA3 for yeast); recombinational cloning.
Comments: This is a bacteria-yeast shuttle vector useful for expressing cDNAs in yeast that includes an auxotrophic marker for selection in yeast.
Size (bp): 8121
Parent Vector: Name: YCplac33
Description: Parent vector is a CEN4, URA3, ampR, ColE1 vector from R.D. Geitz described in Gene 74(2) 5270534 (1988). The Gal1-10 promoter and the Gateway-type attR recombination cassette (reference frame A, see information at the Invitrogen website) were inserted into the parent to create pBY011.
Empty Vector: None
Properties: Gateway, acceptor (destination), inducible expression, recombinational cloning, with tag/fusion/marker, yeast expression, yeast shuttle
Author Name: Bhullar
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin 3651 4333
negative selection marker ccdB ccdB negative selection marker gene LOST in recombined (with insert) form 4915 5124
primer M13 reverse M13 reverse sequencing primer 4702 4722
primer M13 forward M13 forward sequencing primer 7183 7200
promoter Gal1 Gal1-10 yeast promoter 6934 7145
promoter Ura3 promoter URA3 promoter 2349 2378
recombination site attR2 attR recombination site 2, lost or altered in recombined (with insert) form 4749 4764
recombination site attR1 attR recombination site 1, lost or altered in recombined (with insert) form 6439 6455
selectable marker Ura3 URA3 1460 2263
selectable marker CmR chloramphenicol resistance gene LOST in recombined (with insert) form 5566 6222
selectable marker AmpR ampicillin resistance gene 2894 3553
yeast centromere CEN yeast centromeric sequence 255 7396
yeast origin ARS1 ARS1 yeast origin of replication 0 0

 

  • Gene
  • Protein
  • Clone
  • Organism

Gene

Target GenBank: AAB64499

 

Protein

SMART domain : Sec7 : Sec7 domain

Clone

Cloning Information : 262667
HIP Master Clone ID : 125142
Original Clone ID : FLH262667.01X

Organism


Species Specific ID: YEL022W