Vector Name:
pLP-DS3xFlag
Synonyms:
pLP-DS-3xFlag
Sequencing Primer:
Forward:
M13F
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, with CITE region, adds 3xFlag tag; amp resistance; recombinational cloning.
Comments:
None
Size (bp):
3719
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1004
1686
primer site
M13 F
M13 Forward primer binding site
3679
3696
promoter
CITE
CITE region for in vitro translation with mammalian extracts
16
518
recombination site
LoxP
LoxP site for recombinational cloning
594
627
selectable marker
AmpR
ampicillin resistance gene
1784
2443
ssDNA origin
f1 ori
f1 origin for ssDNA production
3090
3530
tag
Flag
3xFlag tag
522
587
trxn termination sequence
T7 term
T7 terminator sequence
776
822
