Vector Name:
pENTR/D-TOPO
Synonyms:
pENTR-D-TOPO
Description:
Gateway type (lambda att-type) recombinational cloning system entry (master) vector for TOPO cloning of insert; kanamycin resistance; recombinational cloning, TOPO cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://www.orfeomecollaboration.org/html/vectors.shtml.
Size (bp):
2580
Parent Vector:
None
Properties:
Gateway, TOPO Cloning, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (NotI and AscI sites flank the TOPO cloning site)
680
693
TOPO recognition site
TOPO 1
TOPO recognition site 1
680
684
TOPO recognition site
TOPO 2
TOPO recognition site 2
689
693
bacterial origin
pUC ori
pUC-type origin of replication
1904
2577
primer site
M13 F
M13 forward priming site
537
552
primer site
M13 Rev
M13 reverse primer site
845
861
promoter
T7
T7promoter and primer binding site
821
840
recombination site
attL1
attL1 recombination site
569
668
recombination site
attL2
attL2 recombination site
705
804
selectable marker
KanR
kanamycin resistance gene
974
1783
trxn termination sequence
rrnB trxn term
rrnB T2 transcription termination sequence
268
295
trxn termination sequence
rrnB txn term
rrnB T1 transcription termination sequence
427
470
