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Detailed Vector Information: pENTR/D-TOPO


Vector Name: pENTR/D-TOPO
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Synonyms: pENTR-D-TOPO


Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning system entry (master) vector for TOPO cloning of insert; kanamycin resistance; recombinational cloning, TOPO cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://www.orfeomecollaboration.org/html/vectors.shtml.
Size (bp): 2580
Empty Vector: None
Parent Vector: None
Properties: Gateway, TOPO Cloning, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (NotI and AscI sites flank the TOPO cloning site) 680 693
TOPO recognition site TOPO 1 TOPO recognition site 1 680 684
TOPO recognition site TOPO 2 TOPO recognition site 2 689 693
bacterial origin pUC ori pUC-type origin of replication 1904 2577
primer site M13 Rev M13 reverse primer site 845 861
primer site M13 F M13 forward priming site 537 552
promoter T7 T7promoter and primer binding site 821 840
recombination site attL1 attL1 recombination site 569 668
recombination site attL2 attL2 recombination site 705 804
selectable marker KanR kanamycin resistance gene 974 1783
trxn termination sequence rrnB trxn term rrnB T2 transcription termination sequence 268 295
trxn termination sequence rrnB txn term rrnB T1 transcription termination sequence 427 470