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Detailed Vector Information: pDB.His.MBP

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Vector Name: pDB.His.MBP
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Synonyms: pDBHMBP, pHM

pDB.His.MBP

Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector that adds 6xHis tag, MBP, TEV protease site; kanamycin resistance; restriction enzyme cloning.
Comments: None
Size (bp): 6494
Empty Vector: EvNO00085130
Parent Vector: None
Properties: bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name: Berkeley Structural Genomics Center
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type bacterial origin of replication 1421 2103
promoter T7 T7 promoter 4984 5003
protease cleavage site TEV TEV protease cleavage site 6232 6249
repressor binding site lac operator lacO sequence region 5003 5025
repressor protein gene lacI lacI coding region 3518 4597
selectable marker KanR kanamycin resistance gene 563 1375
ssDNA origin f1 ori f1 origin 29 335
tag MBP Maltose Binding Protein cds 5071 6369
tag His 6xHis tag 5083 5100
trxn termination sequence T7 term T7 terminator sequence 6423 6469