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Detailed Vector Information: pDB.His.TRX


Vector Name: pDB.His.TRX
       DyNA Vector Map

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Synonyms: pHX


Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector that adds N-terminal 6xHis tag, thioredoxin and TEV protease sites; kanamycin resistance; restriction enzyme cloning.
Comments: None
Size (bp): 5720
Empty Vector: EvNO00085132
Parent Vector: None
Properties: bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name: Berkeley Structural Genomics Center
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type bacterial origin of replication 1421 2103
promoter T7 T7 promoter 4984 5003
protease cleavage site TEV TEV protease cleavage site 5458 5475
repressor binding site lac operator lacO sequence region 5003 5025
repressor protein gene lacI lacI coding region 3518 4597
restriction site CpoI CpoI restriction site 5202 5208
selectable marker KanR kanamycin resistance gene 563 1375
ssDNA origin f1 ori f1 origin 29 335
tag TRX thioredoxin tag 5107 5430
tag His 6xHis tag 5083 5100
trxn termination sequence T7 term T7 terminator sequence 5649 5695