Detailed Vector Information: pMCSG20

Vector Name:
pMCSG20
Synonyms:
pMCSG-20
Sequencing Primer:
Forward:  T7
Reverse:  T7 terminator
Description:
Coexpression vector with T7 promoter, adds N-terminal Stag, GST, TEV, SNA; amp resistance; ligation independent cloning (LIC
Comments:
pMCSG7 derivative
Size (bp):
5991
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
PMID: 17363272
Title: New vectors for co-expression of proteins: structure of Bacillus subtilis ScoAB obtained by high-throughput protocols
PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
MCS
LIC
Ligation independent cloning (LIC) site
204
235
bacterial origin
ori
ColE1-type bacterial origin of replication
3985
3985
promoter
T7
T7 promoter
1065
1081
protease cleavage site
TEV
TEV cleavage site cds
223
243
repressor binding site
lac operator
LacO fragment
1040
1062
repressor protein gene
LacI
LacI coding sequence
1595
2554
selectable marker
AmpR
ampicillin resistance gene
4743
5603
tag
His
C-terminal 6xHis tag
140
157
tag
S-tag
N-terminal S-tag
946
990
tag
GST
Glutathione transferase (GST) tag
265
924
trxn termination sequence
T7 term
T7 terminator sequence
26
72