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Detailed Vector Information: pMCSG20

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Vector Name: pMCSG20
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Synonyms: pMCSG-20

pMCSG20

Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Coexpression vector with T7 promoter, adds N-terminal Stag, GST, TEV, SNA; amp resistance; ligation independent cloning (LIC
Comments: pMCSG7 derivative
Size (bp): 5991
Empty Vector: EvNO00084266
Parent Vector: None
Properties: bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: PMID: 17363272
Title: New vectors for co-expression of proteins: structure of Bacillus subtilis ScoAB obtained by high-throughput protocols

PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS LIC Ligation independent cloning (LIC) site 204 235
bacterial origin ori ColE1-type bacterial origin of replication 3985 3985
promoter T7 T7 promoter 1065 1081
protease cleavage site TEV TEV cleavage site cds 223 243
repressor binding site lac operator LacO fragment 1040 1062
repressor protein gene LacI LacI coding sequence 1595 2554
selectable marker AmpR ampicillin resistance gene 4743 5603
tag S-tag N-terminal S-tag 946 990
tag GST Glutathione transferase (GST) tag 265 924
tag His C-terminal 6xHis tag 140 157
trxn termination sequence T7 term T7 terminator sequence 26 72