Vector Name:
pNYSGXRC
Synonyms:
pNYSGXRC_NY
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial (E. coli) expression vector for directionaly cloning, similar to BS vector; kanamycin resistance; directional cloning (restriction enzyme cloning).
Comments:
Supplied by Chris Lima for the NYSGXRC.
Size (bp):
5759
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColE1-type origin of replication
1554
2236
promoter
T7
T7 promoter (for Smt2p region)
5117
5136
repressor protein gene
LacI
LacI coding region
3651
4730
selectable marker
AmpR
ampicillin resistance gene
797
1456
ssDNA origin
F1 ori
F1 origin for ssDNA production
457
17
tag
His
6xHis tag
5216
5233
tag
Smt3
S. cerevisiae Smt3p cds
5204
5584
trxn regulatory element
lac operator
lac operator
5136
5158
trxn termination sequence
T7 term
T7 terminator sequence (after Smt3p region)
5688
5734
