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Detailed Vector Information: pNYSGXRC

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Vector Name: pNYSGXRC
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Synonyms: pNYSGXRC_NY

pNYSGXRC

Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector for directionaly cloning, similar to BS vector; kanamycin resistance; directional cloning (restriction enzyme cloning).
Comments: Supplied by Chris Lima for the NYSGXRC.
Size (bp): 5759
Empty Vector: None
Parent Vector: None
Properties: bacterial expression, multiple cloning site
Author Name: New York SGX Research Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type origin of replication 1554 2236
promoter T7 T7 promoter (for Smt2p region) 5117 5136
repressor protein gene LacI LacI coding region 3651 4730
selectable marker AmpR ampicillin resistance gene 797 1456
ssDNA origin F1 ori F1 origin for ssDNA production 457 17
tag His 6xHis tag 5216 5233
tag Smt3 S. cerevisiae Smt3p cds 5204 5584
trxn regulatory element lac operator lac operator 5136 5158
trxn termination sequence T7 term T7 terminator sequence (after Smt3p region) 5688 5734