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Detailed Vector Information: pMCSG19C


Vector Name: pMCSG19C
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Synonyms: ''


Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Vector for solubility of insert and for purification, with T7 promoter, adds N-terminal MBP, TVMV, His, TEV, NA; amp resistance; ligation independent cloning (LIC).
Comments: as 19 + constitutive expression of TVMV protease from integrated gene
Size (bp): 7468
Empty Vector: EvNO00084274
Parent Vector: None
Properties: in vitro transcription, ligation independent cloning (LIC)
Author Name: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 5432 5432
gene fragment TVMV cds TVMV protease gene 1927 2637
ligation independent cloning LIC LIC Site 204 235
promoter T7 T7 promoter 1515 1531
protease cleavage site TEV protease cleavage site 223 243
protease cleavage site TVMV TVMV protease cleavage site 286 306
repressor binding site lac operator LacO fragment 1490 1512
repressor binding site tet operator TetO fragment 1753 1908
repressor protein gene LacI LacI CDS 3072 4031
selectable marker AmpR ampicillin resistance gene 6220 7080
tag His N-terminal 6x His tag 268 285
tag MBP N-terminal MBP tag 337 1443
trxn termination sequence T7 term T7 terminator 26 72