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Detailed Vector Information: pVP65K


Vector Name: pVP65K
       DyNA Vector Map

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Synonyms: ''


Sequencing Primer: Forward:  MBP Forward
Reverse:  Unknown
Description: Bacterial (E. coli) expression with N-terminal 8xHis, MBP tag; kanamycin resistance in bacteria; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat cassette.
Comments: Vector contains a lethal BarCat cassette that requires the strain BR610 for growth. Lethal cassette is removed during cloning.
Size (bp): 7174
Empty Vector: EvNO00306668
Parent Vector: None
Properties: bacterial expression
Author Name: University of Wisconsin
Center for Eukaryotic Structural Genomics
Publications: PMID: 18988018
Title: Flexi vector cloning.

Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin of replication 4977 6204
gene fragment TVMV cds TVMV gene 139 849
gene fragment BarCat BarCat cassette including Barnase 2245 3376
primer MBP forward MBP forward primer 2071 2091
primer T7 term T7 terminator reverse primer 3426 3443
promoter T5 T5 promoter region 952 1032
promoter TET TET promoter 873 946
promoter LacIq LacIq-wt promoter 4899 4967
protease cleavage site TVMV TVMV protease cleavage site 2189 2209
repressor binding site LacI LacI coding region 3807 4898
selectable marker KanR Kanamycin resistance gene 6213 7174
tag MBP N-terminal maltose binding protein tag 1061 2165
tag His 8xHis tag 2210 2234
trxn termination sequence rrnB rrnB transcription terminator 15 111