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Detailed Vector Information: pH3c-LIC

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Vector Name: pH3c-LIC
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Synonyms: None

pH3c-LIC

Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with N-terminal 8x His tag separated from the insert by a 3C protease cleavage site; kanamycin resistance in bacteria; ligation independent cloning (LIC)
Comments: None
Size (bp): 5382
Empty Vector: EvNO00292960
Parent Vector: None
Properties: bacterial expression
Author Name: Center for Structures of Membrane Proteins
University of California San Francisco
Marta Pioletti
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
ligation independent cloning LIC Ligation independent cloning site 5162 5167
phage origin f1 f1 phage origin 29 335
promoter T7 T7 promoter 4984 5003
protease cleavage site 3C 3C protease cleavage site; separates the His tag from the insert 5132 5155
selectable marker KanR Kanamycin resistance gene 563 1066
tag His N-terminal 8xHis tag 5075 5098
trxn termination sequence T7 term T7 transcription termination sequence 5316 5362