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Detailed Vector Information: pSGP47

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Vector Name: pSGP47
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Synonyms: None

pSGP47

Sequencing Primer: Forward:  pSGP-Forward
Reverse:  pSGP-Reverse
Description: Yeast expression vector with Adh2 promoter, adds 3C protease cleavage site and 10xHis tag; ampicillin resistance (bacteria); uracil (yeast); ligation independent cloning (LIC).
Comments: S. cerevisiae expression strain
Size (bp): 6296
Empty Vector: EvNO00304417
Parent Vector: None
Properties: ligation independent cloning (LIC), with tag/fusion/marker, yeast expression
Author Name: Center for High Throughput Structural Biology
Mark Dumont
Nadia Fedoriw
PSI
Publications: PMID: 20045057
Title: Purification of transmembrane proteins from Saccharomyces cerevisiae for X-ray crystallography

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
ligation independent cloning LIC Ligation independent cloning site 4220 4231
primer primer sequencing primer: CAATCAACTATCAACTATTAACTA 4155 4178
promoter Ura3 promoter Ura3 promoter 5981 6010
protease cleavage site 3C Rhinovirus 3C protease cleavage site 4233 4256
selectable marker AmpR ampicillan selectable marker 1742 2401
selectable marker Ura3 URA3 nutritional marker 5092 5895
tag His 10xHis tag 4275 4304