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Detailed Vector Information: pMA507

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Vector Name: pMA507
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Synonyms: None

pMA507

Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: E. coli expression vector with a T7 promoter, N-terminal 6xHis tag and TEV protease cleavage site; contains the ccdB death cassette; kanamycin resistance in bacteria; ligation independent cloning (LIC).
Comments: Requires special host strain E. coli DB3.1 for growth
Size (bp): 6046
Empty Vector: EvNO00305686
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Integrated Center for Structure and Function Innovation
Mark Arbing
David Eisenberg
PSI
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial operon lac operator Lac operator CDS 5003 5027
bacterial origin ColE ColE1 pBR322 origin 2084 2084
death cassette ccdB ccdB death cassette 5134 5883
phage origin f1 f1 phage origin 12 467
primer T7 forward T7 forward sequencing primer 4985 5001
primer T7 term primer T7 terminator reverse primer 5963 5981
promoter T7 T7 promoter 4984 5000
protease cleavage site TEV TEV protease cleavage site 5113 5133
repressor protein gene LacI lacI regulatory gene 3518 4597
ribosome binding site RBS ribosomal binding site 5055 5063
selectable marker KanR kanamycin resistance gene 563 1375
tag His N terminal 6xHis tag 5095 5112
trxn termination sequence T7 term T7 terminator 5975 6021