Detailed Vector Information: pLenti6.2/V5-DEST

Vector Name:
pLenti6.2/V5-DEST
Synonyms:
pLenti6.2/V5-DEST
Sequencing Primer:
Forward:  CMV forward
Reverse:  V5 reverse
Description:
with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection, mammalian expression expression with CMV and PGK and BlastR and AmpR and RSV and 5' splice donor and HIV-1 psi and HIV-1 RRE and 3' splice acceptor and V5 and SV40 and pUC ori and attR1 and attR2 and ccdB and CmR; blasticidin resistance in mammalian, ampicillin resistance in bacterial;
Comments:
None
Size (bp):
8813
Parent Vector:
None
Properties:
Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
bacterial origin
pUC ori
pUC origin of replication
7733
8406
death cassette
ccdB
ccdB death cassette (removed when insert is present)
3767
3976
packaging signal
HIV-1 psi
HIV-1 psi packaging signal
521
565
poly-A signal
SV40
SV40 polyadenylation signal
5639
5770
promoter
CMV
CMV promoter driving gene of interest
1809
2392
promoter
PGK
PGK promoter to drive selection marker in mammalian cells
4266
4771
promoter
RSV
RSV/5' LTR hybrid promoter
1
410
recombination site
attR1
attR1 recombinational cloning site
2440
2456
recombination site
attR2
attR2 recombinational cloning site
4127
4142
response element
HIV-1 RRE
HIV-1 Rev response element (RRE)
1075
1308
selectable marker
BlastR
Blasticidin resistance gene in mammalian cells
4849
5249
selectable marker
CmR
chloramphenicol resistance marker (removed in plasmids with insert)
2673
3329
selectable marker
AmpR
Ampicillin resistance gene for selection in bacteria
6728
7588
splice acceptor site
3' splice acceptor
two 3' splice acceptors at 1656 and 1684
1656
1656
splice donor site
5' splice donor
5' splice donor
520
520
tag
V5
C-terminal V5 epitope tag
4197
4238