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Detailed Vector Information: pMYC4-H


Vector Name: pMYC4-H
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Synonyms: ''


Sequencing Primer: Forward:  Phsp
Reverse:  Unknown
Description: Mycoplasma coexpression expression vector with the Phsp promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning ( NdeI, SbfI).
Comments: Target gene controlled by Phsp promoter for expression is cloned between NdeI and SbfI restriction sites.
Size (bp): 5165
Empty Vector: EvNO00306565
Parent Vector: None
Properties: multiple cloning site, with tag/fusion/marker
Author Name: Brian Fox
Yong Chang
David Mead
Vinay Dhodda
Phil Brumm
Center for Eukaryotic Structural Genomics
Publications: PMID: 19760663
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies

Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
MCS MCS Multiple cloning site 465 571
bacterial origin ColE ColE1 replication origin (on complementary strand) 1250 1784
mycobacterial origin myc ori mycobacterial origin of replication 1971 3816
protease cleavage site Phsp Phsp promoter 85 464
selectable marker HygR hygromycin resistance cassette 4153 5151
tag His His tag 508 540
tag myc C-terminal c-myc tag 508 543
trxn termination sequence rrnB rrnB T2 transcriptional terminator 50 70
trxn termination sequence T4g32 T4g32 transcription terminator 586 606