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Detailed Vector Information: pMYC5-H

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Vector Name: pMYC5-H
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Synonyms: None

pMYC5-H

Sequencing Primer: Forward:  Phsp
Reverse:  Unknown
Description: Mycoplasma coexpression expression vector with the Phsp promoter and C-termial c-myc tag; modified from pMYC4-H with unique SrfI site; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (NdeI, SbfI).
Comments: Target gene controlled by Phsp promoter for expression is cloned between NdeI and SbfI restriction sites. Modified from PMYC4H with unique SrfI site.
Size (bp): 5157
Empty Vector: EvNO00306566
Parent Vector: None
Properties: multiple cloning site, with tag/fusion/marker
Author Name: Center for Eukaryotic Structural Genomics
Brian Fox
Phil Brumm
Yong Chang
David Mead
Vinay Dhodda
PSI
Publications: PMID: 19760663
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS multiple cloning site 457 563
bacterial origin ColE ColE1 replication origin (on complementary strand) 1242 1776
mycobacterial origin myc ori mycobacterial origin of replication 1963 3808
promoter Phsp Phsp promoter 77 456
selectable marker HygR hygromycin resistance cassette 4145 5143
tag His C-terminal His tag 500 532
tag myc C-terminal c-myc tag 500 535
trxn termination sequence T4g32 T4g32 transcription terminator 578 598
trxn termination sequence rrnB rrnB T2 transcriptional terminator 42 62