Detailed Vector Information: pVP91A

Vector Name:
pVP91A
Synonyms:
''
Sequencing Primer:
Forward:  Unknown
Reverse:  Unknown
Description:
Bacterial expression vector with a T5 promoter and N-terminal 8xHis tag; ampicilliin resistance in bacteria; restriction enzyme cloning.
Comments:
Cloning into this vector will remove the sacB/CAT cassette and allow growth in the presence of 5% sucrose; empty vector should be proagated in dam- E. coli strain.
Size (bp):
8538
Parent Vector:
None
Properties:
bacterial expression, with tag/fusion/marker
Author Name:
Center for Eukaryotic Structural Genomics
PSI
Publications:
None
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 bacterial origin of replication
4359
5586
gene fragment
sacB
sucrase gene from B. subtillis
496
1920
gene fragment
CAT
chloramphenicol acetyltransferase gene
2094
2748
promoter
T5
T5 promoter region
122
202
repressor protein gene
LacI
LacI-wt coding region (on complementary strand)
3189
4280
selectable marker
AmpR
ampicillin resistance cassette (on complementary strand)
5593
6452
tag
His
N-terminal 8xHis tag
231
262
trxn termination sequence
T7 term
T7 terminator reverse (on complementary strand)
2808
2825
trxn termination sequence
rrnB
rrnB transcription termination
3005
3101