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Detailed Vector Information: pVP91A


Vector Name: pVP91A
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Synonyms: ''


Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: Bacterial expression vector with a T5 promoter and N-terminal 8xHis tag; ampicilliin resistance in bacteria; restriction enzyme cloning.
Comments: Cloning into this vector will remove the sacB/CAT cassette and allow growth in the presence of 5% sucrose; empty vector should be proagated in dam- E. coli strain.
Size (bp): 8538
Empty Vector: EvNO00306666
Parent Vector: None
Properties: bacterial expression, with tag/fusion/marker
Author Name: Center for Eukaryotic Structural Genomics
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin of replication 4359 5586
gene fragment sacB sucrase gene from B. subtillis 496 1920
gene fragment CAT chloramphenicol acetyltransferase gene 2094 2748
promoter T5 T5 promoter region 122 202
repressor protein gene LacI LacI-wt coding region (on complementary strand) 3189 4280
selectable marker AmpR ampicillin resistance cassette (on complementary strand) 5593 6452
tag His N-terminal 8xHis tag 231 262
trxn termination sequence T7 term T7 terminator reverse (on complementary strand) 2808 2825
trxn termination sequence rrnB rrnB transcription termination 3005 3101