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Detailed Vector Information: pEU-HSBC


Vector Name: pEU-HSBC
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Synonyms: ''


Sequencing Primer: Forward:  His2 Forward
Reverse:  T7 terminator
Description: In Vitro SP6 transcription and wheat germ cell free protein expression vector with a SP6 promoter and N-terminal 6xHis tag; ampicillin resistance in bacteria; restriction enzyme cloning.
Comments: Cloning into this vector will remove the sacB/CAT cassette and allow growth in the presence of 5% sucrose; empty vector should be proagated in dam- E. coli strain
Size (bp): 6336
Empty Vector: EvNO00306667
Parent Vector: None
Properties: cell-free expression, in vitro transcription, with tag/fusion/marker
Author Name: Center for Eukaryotic Structural Genomics
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
gene fragment sacB sucrase gene from B. subtillis 790 2209
gene fragment CAT chloramphenicol acetyltransferase gene 2381 3037
primer His2 forward His2 forward sequencing primer 424 449
primer T7 reverse T7 terminator reverse sequencing primer 3250 3270
promoter SP6 SP6 promoter 373 392
selectable marker AmpR ampicillin resistance cassette (on complementary strand) 4956 5816
tag His N-terminal 6xHis tag 527 544
trln enhancer trln enhancer TMV-Omega translational enhancer 398 520