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Detailed Vector Information: pMCSG32

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Vector Name: pMCSG32
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Synonyms: None

pMCSG32

Sequencing Primer: Forward:  T7short
Reverse:  T7 terminator
Description: Bacterial expression vector with T7 promoter and C-terminal 6xHis, TEV-protease cleavage site, N-terminal MBP tag with TVMV site; ampicillin resistance in bacterial; ligation independent cloning (LIC)
Comments: None
Size (bp): 6418
Empty Vector: EvNO00340965
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: PSI
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
Publications: PMID: 20213425
Title: Cleavable C-terminal His-tag vectors for structure determination.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin of replication 4412 4412
primer T7 reverse T7 terminator reverse sequencing primer 66 83
primer T7 forward T7 forward sequencing primer 1491 1507
promoter T7 T7 promoter 1492 1508
protease cleavage site TVMV TVMV cleavage site 263 283
protease cleavage site TEV TEV protease cleavage site 232 252
recombination site LIC LIC site 245 265
repressor binding site lac operator LacO fragment 1467 1489
repressor protein gene LacI Lac I cds 2022 2981
selectable marker AmpR ampicillin resistance marker 5170 6030
tag His 2 His Tag;carboxy terminal His tag from pET-21a 140 157
tag His C-terminal 6xHis tag 208 225
tag MBP N-terminal maltose binding protein (MBP) tag 314 1420
trxn termination sequence T7 term T7 terminator 26 72