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Detailed Vector Information: pCPD_nSBP-Halo_DC

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Vector Name: pCPD_nSBP-Halo_DC
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Synonyms: pCPD10_DC

pCPD_nSBP-Halo_DC

Sequencing Primer: Forward:  pCPD5698_F
Reverse:  pCPD6268_R
Description: Bacterial expression vector with a T7 promoter and N-terminal SBP and Halo tags, seperated by protease cleavage sites for thrombin and TEV protease; ampicillin resistance in bacteria; Gateway cloning
Comments: One in a series of pCPD vectors made by J. Saul for E. coli protein expression at CPD.
Size (bp): 7944
Empty Vector: EvNO00413803
Parent Vector: None
Properties: Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name: Joshua LaBaer
Center for Personalized Diagnostics
Ji Qiu
Justin Saul
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin 1344 2026
death cassette ccdB ccdB death cassette (removed when insert present) 6098 7752
death cassette 2 ccdB (BsrGI removed) ccdB death cassette with BsrGI restriction site removed (removed in plasmids with insert) 7307 7612
primer pCPD6268_R reverse sequencing primer (5' agccaactcagcttccttt 3') 7832 7850
primer HaloseqF Forward sequencing primer (5' aagcctgcctaactgcaa 3') 5913 5930
promoter T7 T7 promoter 4911 4927
protease cleavage site Thrombin Thrombin protease cleavage site 5199 5136
protease cleavage site TEV TEV protease cleavage site (Promega EDLYYFQS) 6046 6066
recombination site attR1 recombination site 6073 6097
recombination site attR2 recombination site 7753 7777
repressor protein gene LacI LacI repressor 3438 4397
ribosome binding site RBS pET ribosomal binding site 4984 4990
selectable marker AmpR ampicillin resistance gene 587 1246
selectable marker CmR chloramphenicol resistance (removed when insert present) 6306 6962
tag HALO N-terminal HALO tag 5143 6033
tag SBP N-terminal Streptavidin-Binding Peptide tag 4999 5112
trxn regulatory element lac operator Lac operator 4930 4952
trxn termination sequence T7 term T7 transcriptional terminator 7872 7919