Vector Name:
pCPD_nSBP-Halo_DC
Synonyms:
pCPD10_DC
Sequencing Primer:
Forward:
pCPD5698_F
Reverse:
pCPD6268_R
Description:
Bacterial expression vector with a T7 promoter and N-terminal SBP and Halo tags, seperated by protease cleavage sites for thrombin and TEV protease; ampicillin resistance in bacteria; Gateway cloning
Comments:
One in a series of pCPD vectors made by J. Saul for E. coli protein expression at CPD.
Size (bp):
7944
Parent Vector:
None
Properties:
Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Ji Qiu
Justin Saul
Center for Personalized Diagnostics
Ji Qiu
Justin Saul
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
1344
2026
death cassette
ccdB
ccdB death cassette (removed when insert present)
6098
7752
death cassette 2
ccdB (BsrGI removed)
ccdB death cassette with BsrGI restriction site removed (removed in plasmids with insert)
7307
7612
primer
HaloseqF
Forward sequencing primer (5' aagcctgcctaactgcaa 3')
5913
5930
primer
pCPD6268_R
reverse sequencing primer (5' agccaactcagcttccttt 3')
7832
7850
promoter
T7
T7 promoter
4911
4927
protease cleavage site
Thrombin
Thrombin protease cleavage site
5199
5136
protease cleavage site
TEV
TEV protease cleavage site (Promega EDLYYFQS)
6046
6066
recombination site
attR1
recombination site
6073
6097
recombination site
attR2
recombination site
7753
7777
repressor protein gene
LacI
LacI repressor
3438
4397
ribosome binding site
RBS
pET ribosomal binding site
4984
4990
selectable marker
AmpR
ampicillin resistance gene
587
1246
selectable marker
CmR
chloramphenicol resistance (removed when insert present)
6306
6962
tag
SBP
N-terminal Streptavidin-Binding Peptide tag
4999
5112
tag
HALO
N-terminal HALO tag
5143
6033
trxn regulatory element
lac operator
Lac operator
4930
4952
trxn termination sequence
T7 term
T7 transcriptional terminator
7872
7919
