Detailed Vector Information: pMCSG49

Vector Name:
pMCSG49
Synonyms:
''
Sequencing Primer:
Forward:  T7 short
Reverse:  T7 terminator
Description:
Bacterial expression vector with a T7 promoter and N-terminal 6xHis tag, TEV cleavable; ampicillin resistance in bacteria; Ligation Independent cloning
Comments:
pMCSG7 digested with PshAI and BsaAI to remove a 1008 bp fragment containing the rop repressor gene and then re-ligated. Origin of replication now similar to the pUC vectors.
Size (bp):
4278
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 origin of replication
2271
2859
ligation independent cloning
LIC
LIC site
204
235
primer
forward
forward sequencing primer TAATACGACTCACTATAGGG
357
376
promoter
T7
T7 promoter
360
376
protease cleavage site
TEV
TEV protease cleavage site
223
243
repressor binding site
lac operator
LacO fragment
335
357
repressor protein gene
LacI
LacI CDS
890
1849
selectable marker
AmpR
Ampicillin resistance gene
3030
3890
tag
His
N-terminal 6xHis tag
268
285
trxn termination sequence
T7 term
T7 transcription termination sequence
26
72