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Detailed Vector Information: pMCSG55


Vector Name: pMCSG55
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Synonyms: None


Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with T7 promoter and N-terminal 6xHis and HA tags followed by TEV protease cleavage site; ampicillin resistance in bacteria; ligation independent cloning
Comments: origin of replication similar to pUC vectors
Size (bp): 4305
Empty Vector: EvNO00454846
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR332 origin of replication 2298 2886
ligation independent cloning LIC ligation independent cloning site 204 235
promoter T7 T7 promoter 387 403
protease cleavage site TEV TEV protease cleavage site 223 243
repressor binding site lac operator LacO fragment 362 384
repressor protein gene LacI Lac I 917 1876
selectable marker AmpR ampicillin resistance 3057 3917
tag His N-terminal 6xHis tag 295 312
tag HA N-terminal HA tag 250 276
trxn termination sequence T7 term T7 terminator 26 72