Detailed Vector Information: pMCSG55

Vector Name:
pMCSG55
Synonyms:
''
Sequencing Primer:
Forward:  T7
Reverse:  T7 terminator
Description:
Bacterial expression vector with T7 promoter and N-terminal 6xHis and HA tags followed by TEV protease cleavage site; ampicillin resistance in bacteria; ligation independent cloning
Comments:
origin of replication similar to pUC vectors
Size (bp):
4305
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR332 origin of replication
2298
2886
ligation independent cloning
LIC
ligation independent cloning site
204
235
promoter
T7
T7 promoter
387
403
protease cleavage site
TEV
TEV protease cleavage site
223
243
repressor binding site
lac operator
LacO fragment
362
384
repressor protein gene
LacI
Lac I
917
1876
selectable marker
AmpR
ampicillin resistance
3057
3917
tag
His
N-terminal 6xHis tag
295
312
tag
HA
N-terminal HA tag
250
276
trxn termination sequence
T7 term
T7 terminator
26
72