Vector Name:
pMCSG56
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with T7 promoter and N-terminal 6xHis and double HA tags followed by TEV protease cleavage site; ampicillin resistance in bacteria; ligation independent cloning
Comments:
origin of replication similar to pUC vectors
Size (bp):
4332
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
PSI:Biology
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR bacterial origin of replication
2325
2913
ligation independent cloning
LIC
ligation independent cloning site
204
235
promoter
T7
T7 promoter
414
430
protease cleavage site
TEV
TEV protease cleavage site
223
243
repressor protein gene
LacI
Lac I
944
1903
selectable marker
AmpR
ampicillin resistance gene
3084
3944
tag
His
N-terminal 6xHis tag
322
339
tag
HA
N-terminal double HA tag
250
303
trxn regulatory element
lac operator
lac operator sequence
389
411
trxn termination sequence
T7 term
T7 terminator
26
72
