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Detailed Vector Information: pJFT7_cHalo-3xFlag_DC


Vector Name: pJFT7_cHalo-3xFlag_DC
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Synonyms: ''


Sequencing Primer: Forward:  NAP150
Reverse:  cHaloseqR
Description: In vitro expression vector with a T7 promoter and C-terminal TEV-cleavable HALO-3xFlag tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments: None
Size (bp): 6274
Empty Vector: EvNO00595408
Parent Vector: None
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning
Author Name: Arizona State University
Joshua LaBaer
Justin Saul
Ji Qiu
Fernanda Festa
Center for Personalized Diagnostics
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin 3559 4241
bacterial origin M13 Ori M13 origin 5365 6090
death cassette ccdB Gateway ccdB death Cassette (DC) (removed in plasmids with inserts) 556 2210
death cassette 2 ccdB (BsrGI removed) ccdB (BsrGI removed) 1765 2070
enhancer CITE CITE enhancer 388 408
primer NAP150 NAP150F forward sequencing primer cccattgtatgggatctgatc (sequencing starts before the 3xFlag-HALO tag) 388 408
primer reverse primer cHaloseqR reverse sequencing primer CAACATCGACGTAGTGCAT (used for sequencing the gene insert) 2351 2369
promoter T7 T7 promoter 6250 3
protease cleavage site TEV TEV protease cleavage site EDLYFQS (removes C-terminal HALO 3xFlag tag) 2258 2278
recombination site attR1 attR1 recombination site 531 555
recombination site attR2 attR2 recombination site 2211 2235
reporter gene LacZ LacZ alpha gene 6095 6163
selectable marker CmR Chloramphenicol resistance gene (removed in plasmids containing gene inserts) 764 1420
selectable marker AmpR ampicillin resistance gene 4339 4998
ssDNA origin f1 f1 origin 5767 6073
tag Flag C-terminal 3xFlag tag 3179 3247
tag HALO C-terminal Halo tag 2294 3178
trxn termination sequence T7 term T7 transcription terminator 3319 3337