Detailed Vector Information: pJFT7_cHalo-3xHA_DC

Vector Name:
pJFT7_cHalo-3xHA_DC
Synonyms:
''
Sequencing Primer:
Forward:  NAP150
Reverse:  cHaloseqR
Description:
In vitro expression vector with a T7 promoter and C-terminal TEV-cleavable HALO-3xHA tag; ampicillin resistance; Gateway or Flexi cloning compatible
Comments:
None
Size (bp):
6301
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Justin Saul
Ji Qiu
Fernanda Festa
Publications:
None
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3586
4268
bacterial origin
M13 Ori
M13 origin
5662
6117
death cassette
ccdB
ccdB death cassette (removed in plasmids that contain an insert)
1765
2070
enhancer
CITE
CITE enhancer
17
515
primer
NAP150
nap150f forward sequencing primer cccattgtatgggatctgatc
388
408
primer
reverse primer
cHaloseqR reverse sequencing primer CAACATCGACGTAGTGCAT
2351
2369
promoter
T7
T7 promoter
6277
3
protease cleavage site
TEV
TEV protease cleavage site (EDLYFQS)
2258
2278
recombination site
attR1
attR1 recombination site
531
555
recombination site
attR2
attR2 recombinational cloning site
2211
2235
reporter gene
LacZ
LacZ alpha
6122
6190
selectable marker
CmR
chloramphenicol resistance (removed in plasmids containing an insert)
764
1420
selectable marker
AmpR
Ampicillin resistance marker
4366
5025
ssDNA origin
f1
f1 origin
5794
6100
tag
HALO
C-terminal TEV-cleavable HALO-3xHA tag
2294
3178
tag
HA
C-terminal TEV-cleavable HALO-3xHA tag
3182
3274
trxn termination sequence
T7 term
T7 terminator
3346
3364