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Detailed Vector Information: pJFT7_nFlag_cHalo_DC


Vector Name: pJFT7_nFlag_cHalo_DC
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Synonyms: ''


Sequencing Primer: Forward:  NAP150
Reverse:  cHaloseqR
Description: In vitro expression vector with a T7 promoter and N-terminal Flag and C-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments: None
Size (bp): 6238
Empty Vector: EvNO00595405
Parent Vector: None
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Joshua LaBaer
Justin Saul
Ji Qiu
Fernanda Festa
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin 3523 4205
death cassette ccdB (BsrGI removed) ccdB death cassette (removed in plasmids containing an insert) 1798 2103
enhancer CITE CITE enhancer 17 515
primer forward primer nap150f forward sequencing primer cccattgtatgggatctgatc 388 408
primer reverse primer cHaloseqR reverse sequencing primer CAACATCGACGTAGTGCAT 2384 2402
promoter T7 T7 promoter 6214 3
protease cleavage site TEV TEV protease cleavage site 2291 2311
recombination site attR1 attR1 recombinational cloning site 562 588
recombination site attR2 attR2 recombinational cloning site 2244 2268
reporter gene LacZ LAcZ alpha 3059 3127
selectable marker AmpR Ampicillin resistance 4303 4962
selectable marker CmR chloramphenicol resistance (removed in plasmids containing an insert) 797 1453
ssDNA origin f1 f1 origin 5731 6037
tag HALO C-terminal Halo tag 2327 3211
tag Flag N-terminal Flag tag 531 554
trxn termination sequence T7 term T7 transcriptional termination 3294 3341