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Detailed Vector Information: pCPD_nHalo_cHIS_DC


Vector Name: pCPD_nHalo_cHIS_DC
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Synonyms: ''


Sequencing Primer: Forward:  HaloseqF
Reverse:  pCPD6268_R
Description: Bacterial expression with a T7 promoter, N-terminal TEV-cleavable HaloTag and C-terminal 3C protease cleavable 6xHis tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments: One in a series of pCPD vectors designed by J. Saul for E. coli protein expression at CPD.
Size (bp): 7821
Empty Vector: EvNO00621023
Parent Vector: None
Properties: Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name: Arizona State University
Joshua LaBaer
Justin Saul
Ji Qiu
Larissa Taveras
Center for Personalized Diagnostics
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin 1344 2026
death cassette ccdB (BsrGI removed) ccdB death cassette - removed in plasmids with gene insert 7163 7468
primer forward primer Forward sequencing primer HaloseqF aagcctgcctaactgcaa 5769 5786
primer reverse primer Reverse sequencing primer pCPD6268_R agccaactcagcttccttt 7709 7727
promoter T7 T7 promoter 4911 4930
protease cleavage site 3C 3C protease cleavage site 7635 7658
recombination site attR1 attR1 recombination site 5929 5953
recombination site attR2 attR2 recombinational cloning site 7609 7633
repressor binding site lac operator Lac Operator 4930 4953
repressor protein gene LacI LacI gene 2733 4397
selectable marker AmpR ampicillin resistance 587 1246
selectable marker CmR Chloramphenicol resistance - removed in plasmids with gene insert 6162 6818
tag His C-terminal 6xHis tag (can be removed by 3C protease) 7665 7682
tag HALO N-terminal HALO tag (can be removed by TEV protease) 4999 5889
trxn termination sequence T7 term T7 transcription termination 7738 7756