Detailed Vector Information: pCPD_nHalo_cHIS_DC

Vector Name:
pCPD_nHalo_cHIS_DC
Synonyms:
''
Sequencing Primer:
Forward:  HaloseqF
Reverse:  pCPD6268_R
Description:
Bacterial expression with a T7 promoter, N-terminal TEV-cleavable HaloTag and C-terminal 3C protease cleavable 6xHis tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pCPD vectors designed by J. Saul for E. coli protein expression at CPD.
Size (bp):
7821
Parent Vector:
None
Properties:
Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Justin Saul
Ji Qiu
Larissa Taveras
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
1344
2026
death cassette
ccdB (BsrGI removed)
ccdB death cassette - removed in plasmids with gene insert
7163
7468
primer
forward primer
Forward sequencing primer HaloseqF aagcctgcctaactgcaa
5769
5786
primer
reverse primer
Reverse sequencing primer pCPD6268_R agccaactcagcttccttt
7709
7727
promoter
T7
T7 promoter
4911
4930
protease cleavage site
3C
3C protease cleavage site
7635
7658
recombination site
attR1
attR1 recombination site
5929
5953
recombination site
attR2
attR2 recombinational cloning site
7609
7633
repressor binding site
lac operator
Lac Operator
4930
4953
repressor protein gene
LacI
LacI gene
2733
4397
selectable marker
AmpR
ampicillin resistance
587
1246
selectable marker
CmR
Chloramphenicol resistance - removed in plasmids with gene insert
6162
6818
tag
His
C-terminal 6xHis tag (can be removed by 3C protease)
7665
7682
tag
HALO
N-terminal HALO tag (can be removed by TEV protease)
4999
5889
trxn termination sequence
T7 term
T7 transcription termination
7738
7756