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Detailed Vector Information: pJFT7_nHalo_DC(r3)


Vector Name: pJFT7_nHalo_DC(r3)
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Synonyms: ''


Sequencing Primer: Forward:  HaloseqF
Reverse:  pCPD6268_R
Description: In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments: One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp): 6229
Empty Vector: EvNO00621024
Parent Vector: None
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Arizona State University
Joshua LaBaer
Justin Saul
Ji Qiu
Fernanda Festa
Center for Personalized Diagnostics
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin 3514 4196
death cassette ccdB (BsrGI removed) ccdB death cassette - removed in plasmids containing a gene insert 2692 2997
enhancer CITE CITE enhancer 17 515
primer forward primer Forward sequencing primer HaloseqF aagcctgcctaactgcaa 1289 1360
primer reverse primer Reverse sequencing primer pCPD6268_R agccaactcagcttccttt 3206 3224
promoter T7 T7 promoter 6205 6229
protease cleavage site TEV TEV protease cleavage site 1422 1442
recombination site attR1 attR1 recombination site 1458 1482
recombination site attR2 attR2 recombination site 3138 3162
reporter gene LacZ LacZ alpha 6050 6118
selectable marker AmpR ampicillin resistance 4294 4953
selectable marker CmR chloramphenicol resistance - removed in plasmids containing a gene insert 1691 2347
ssDNA origin f1 F1 ssDNA origin 5722 6028
tag HALO N-terminal TEV-cleavable Halo tag 519 1409
trxn termination sequence T7 term T7 transcription termination 3285 3332