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Detailed Vector Information: pET15Nano6HT_NESG

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Vector Name: pET15Nano6HT_NESG
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Synonyms: None

pET15Nano6HT_NESG

Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression withT7 lac promoter, N-terminal Nano-tag, 6XHis-tag, TEV-cleavable; ampicillin resistance in bacteria; restriction enzyme cloning
Comments: None
Size (bp): 5783
Empty Vector: None
Parent Vector: None
Properties: bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name: Northeast Structural Genomics Consortium
Rutgers University
Gaetano T. Montleione
Thomas B. Acton
PSI:Biology
Stephen Anderson
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 origin 3938 4620
promoter T7 T7 promoter 535 554
protease cleavage site TEV TEV protease cleavage site 384 401
repressor binding site lac operator lac operator 513 535
repressor protein gene LacI LacI repressor 944 2023
selectable marker AmpR Ampicillin resistance 4718 5377
tag His N-terminal 6xHis tag 402 419
tag Nano N-termianl streptavidin-binding Nano tag 420 464