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Detailed Vector Information: pRSET-natTEVGFPHis

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Vector Name: pRSET-natTEVGFPHis
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Synonyms: None

pRSET-natTEVGFPHis

Sequencing Primer: Forward:  T7
Reverse:  GFPR
Description: Bacterial expression vector with T7 promoter and C-terminal TEV-cleavavable GFP and 6xHis-tags; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
Comments: ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
Size (bp): 4016
Empty Vector: EvNO00629004
Parent Vector: None
Properties: bacterial expression, fluorescent marker, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: PSI:Biology
Arizona State University
Center for Membrane Proteins in Infectious Diseases
Felicia M. Craciunescu
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 origin 3166 3839
promoter T7 T7 promoter 20 39
protease cleavage site TEV TEV protease cleavage site 532 549
ribosome binding site RBS ribosome binding site 85 92
selectable marker AmpR ampicillin resistance 2161 3021
ssDNA origin f1 f1 origin 1575 2030
tag GFP C-terminal GFP 586 1299
tag His C-terminal 6xHis tag 1303 1320
trxn termination sequence T7 term T7 terminator 1375 1504