Vector Name:
pCMVi-UB
Synonyms:
''
Description:
Vector for antibody production in mice upon genetic immunization by gene gun with CMV promoter and N-terminal mouse ubiqiutin protein with Gly76Ala mutation; ampicillin resistance in bacteria; restriction enzyme cloning
Comments:
The mouse ubiquitin sequence contains the C-terminal Gly76Ala mutation to prevent de-ubiquination. Clone insert in-frame using the BglII restriction sites and another downstream site before the transcription terminator (such as XmaI)
Size (bp):
5218
Parent Vector:
None
Properties:
mammalian expression, multiple cloning site, with tag/fusion/marker
Author Name:
Arizona State University
Stephen Johnston
Kathryn Sykes
Stephen Johnston
Kathryn Sykes
Publications:
PMID:
10433551
Title: Genetic live vaccines mimic the antigenicity but not pathogenicity of live viruses.
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
CoE1 bacterial origin
2894
3750
gene fragment
ubiquitin
N-terminal mouse ubiquitin protein with Gly76Ala mutation
1250
1476
intron
intron
Intron
1028
1160
primer
forward primer
UBFOR sequencing primer AACATCCAGAAGGAGTCA
1427
1444
primer
reverse primer
HGHTER reverse sequencing primer ACTGGAGTGGCAACTTCCAGG
1594
1614
promoter
CMV
CMV promoter
174
915
selectable marker
AmpR
Ampicillin resistance
3760
4670
ssDNA origin
f1
f1 origin
4752
5204
trxn regulatory element
hGH
hGH terminator
1556
2032
