Bacterial expression with T7 promoter and N-terminal E. coli YtfM (aa1-21) signal sequence and 8xHis-tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
Comments:
Signal sequence is aa 1-21 from E. coli YtfM, which was supposedly used in PMID 20060837. Unfortunately, the actual sequence used in this publication was actually YtfM aa 1-27 with the V20A mutation, followed by 6xHis + LE (van den Berg, personal communication)
Size (bp):
3311
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Arizona State University
Felicia M. Craciunescu
Center for Membrane Proteins in Infectious Diseases
PSI:Biology