Detailed Vector Information: pRSET-YtfM-8xHis-N-term

Vector Name:
pRSET-YtfM-8xHis-N-term
Synonyms:
''
Sequencing Primer:
Forward:  T7
Reverse:  T7 terminator
Description:
Bacterial expression with T7 promoter and N-terminal E. coli YtfM (aa1-21) signal sequence and 8xHis-tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
Comments:
Signal sequence is aa 1-21 from E. coli YtfM, which was supposedly used in PMID 20060837. Unfortunately, the actual sequence used in this publication was actually YtfM aa 1-27 with the V20A mutation, followed by 6xHis + LE (van den Berg, personal communication)
Size (bp):
3311
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Arizona State University
Felicia M. Craciunescu
Center for Membrane Proteins in Infectious Diseases
PSI:Biology
Publications:
None
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
2461
3134
promoter
T7
T7 promoter
20
39
ribosome binding site
RBS
ribosome binding site
85
92
selectable marker
AmpR
ampicillin resistance
1456
2316
signal sequence
YtfM
N-terminal E. coli YtfM (aa1-21) signal sequence
100
162
ssDNA origin
f1
f1 origin
870
1325
tag
His
N-terminal 8xHis tag
163
186
trxn termination sequence
T7 term
T7 terminator
670
799