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Detailed Vector Information: pRSET-C8xHis


Vector Name: pRSET-C8xHis
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Synonyms: ''


Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with T7 promoter and C-terminal 8xHis tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
Comments: ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
Size (bp): 3248
Empty Vector: EvNO00629010
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Arizona State University
Felicia M. Craciunescu
Center for Membrane Proteins in Infectious Diseases
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin 2398 3071
promoter T7 T7 promoter 20 39
ribosome binding site RBS ribosomal binding site 85 92
selectable marker AmpR ampicillin resistance 1393 2253
ssDNA origin f1 f1 origin 807 1262
tag His C-terminal 8xHis tag 532 555
trxn termination sequence T7 term T7 terminator 607 736