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Detailed Vector Information: pMK-Dest

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Vector Name: pMK-Dest
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Synonyms: ''

pMK-Dest

Sequencing Primer: Forward:  CMV forward
Reverse:  None
Description: with tag/fusion/marker assay, Gateway® clone, bacterial expression expression with CMV and CMV-F and attR1 and attR2 and lac promoter and pBR322 origin and NeoR and AttB2 and TEV; kanamycin resistance in bacterial, neomycin resistance in bacterial;
Comments: From Karen Anderson Lab, Produced by Bianca Varda, Krishna, and Mark Knappenberger
Size (bp): 3386
Empty Vector: EvNO00877066
Parent Vector: Name: pMax-Cloning
Description:
Properties: Gateway, bacterial expression, with tag/fusion/marker
Author Name: Center for Personalized Diagnostics
Bianca
Mark Knappenberger
Krishna
Publications: None
Vector Map:         Vector Sequence:
Sequence: Not Available

 



Vector Features:

Type Name Description Start Position End Position
CDS TEV TEV Cleavage site 1252 1272
Origin pBR322 origin 2748 2130
primer CMV-F 949 969
promoter CMV 938 1019
promoter lac promoter 1437 1460
recombination site attR1 1288 1412
recombination site attR2 1412 1312
recombination site AttB2 2066 2090
selectable marker NeoR Neomycin/Kanamycin Resistance 2816 209