Detailed Vector Information: pLenti6.3/V5-DEST

Vector Name:
pLenti6.3/V5-DEST
Synonyms:
''
Sequencing Primer:
Forward:  CMV forward
Reverse:  V5 reverse
Description:
with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection, mammalian expression expression with CMV and RSV and 5' splice donor and HIV-1 psi and HIV-1 RRE and 3' splice acceptor and attR1 and V5 and WPRE and 5pLTR; ampicillin resistance in bacterial, chloramphenicol resistance in bacterial, blasticidin resistance in mammalian;
Comments:
pLent6.3/V5-DEST from Invitrogen: https://www.thermofisher.com/order/catalog/product/V53306 Catalog: V53306 For research use only. Not intended for any therapeutic or diagnostic use. The upgrade from pLenti6 to pLenti6.3 is in the addition of a WPRE cassette. Lentiviral Gateway destination vector for expression of a C-terminally V5-tagged protein.
Size (bp):
9387
Parent Vector:
None
Properties:
Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
Miscellaneous Feature
V5
C-terminal V5 tag
4325
4366
packaging signal
HIV-1 psi
HIV-1 psi packaging signal
457
582
promoter
CMV
CMV Promoter
1935
2519
promoter
RSV
RSV promoter
3
229
recombination site
attR1
2568
2692
response element
HIV-1 RRE
HIV-1 Rev response element (RRE)
1075
1308
splice acceptor site
3' splice acceptor
3' splice acceptor
1656
1656
splice donor site
5' splice donor
5' splice donor site
520
520
trln enhancer
WPRE
4385
4982
viral LTR
5pLTR
5' LTR (truncated)
230
410