Vector Name:
pANT7_GST-control
Synonyms:
''
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
In vitro expression vector expressing two GSTs as a control vector for clones in pANT7_cGST; ampicillin resistance in bacteria; recombinational cloning.
Comments:
This vector is a control vector for clones in pANT7_cGST that only expresses GST.
Size (bp):
4982
Parent Vector:
Name:
pANT7_cGST
Description:
Properties:
Gateway, in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Shane Miersch
Center for Personalized Diagnostics
Shane Miersch
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
2267
2949
bacterial origin
f1
f1 single stranded origin
4475
4781
enhancer
CITE
CITE enhancer
17
515
gene fragment
GST-2
GST expressed in tandem with GST from the pANT7_cGST vector
576
1241
promoter
T7
T7 promoter
4966
4982
recombination site
AttB1
attB1 recombinational cloning site
549
573
recombination site
AttB2
attB2 recombinational cloning site
1246
1270
selectable marker
AmpR
ampicillin resistance
3047
3706
tag
GST
GST
1300
1965
trxn termination sequence
T7 term
T7 terminator
2038
2085
