Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00868799
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 2985nts
Open reading frame: 1 to 2985
ATGTCATCATTGCCAAGAAGAGCGAAAGTACAGGTCCAGGATGTGGTACTGAAAGATGAA
TTTTCTTCATTCTCTGAGTTATCATCTGCCTCTGAAGAAGATGACAAGGAAGATAGTGCC
TGGGAGCCCCAAAAGAAAGTTCCCAGAAGCCGTAAACAGCCCCCTCCCAAGGAATCCAAA
CCAAAGAGGATGCCTCGGGTGAAGAAGAATGCCCCACAGATCAGTGATGGCTCAGAAGTC
GTTGTTGTTAAGGAGGAGCTGAATAGCTCTGTGGCTATTGCTGATACTGCTTTAGAAGAC
AGAAAAAATAAATTGGATACTGTACAGACTCTGAAAACAGCCAAGACAAAACAGAAATGT
GCAGCGCAGCCACATACAGTTCGAAGGACTAAAAAGCTGAAAGTTGAAGAAGAAACCAGC
AAAGCCAGCAACTTAGAGGGTGAGAGTAATAGTTCAGAGACACCATCCACAAGCACTGTG
TGGGGAGGTACATGCAAGAAGGAAGAGAATGACGATGACTTTACATTTGGTCAGTCCGCT
TTAAAGAAAATCAAGACTGAGACATATCCTCAGGGGCAGCCTGTCAAGTTTCCAGCAAAT
GCCAATAGTACTAAAGAGGAGGTGGAAATGAATTGGGACATGGTACAGGTTTTATCTGAG
AGAACTAATATTGAACCTTGGGTTTGTGCCAACATCATTCGTCTCTTTAATGATGATAAC
ACAATTCCCTTCATTATACGTTATAGAAAAGAGCTCATTAATAACCTTGATGCTGATTCC
TTGAGAGAAGTTCAGCAAACCCTAGAAGAGCTACGGGCTGTTGCAAAGAAAGTTCATAGT
ACAATCCAGAAAATTAAGAAGGAAGGGAAGATGTCTGAGTGCTTGTTAAAAGCCATGCTG
AATTGTAAAACTTTTGAAGAACTAGAACACGTGTCTGCTCCATATAAAACTGGAAGCAAA
GGGACTAAAGCCCAGAGAGCAAGACAGTTGGGCTTAGAAGGAGCAGCCAGGGCACTGCTT
GAGAAACCAGGGGAGCTCAGTCTGCTATCGTACATTAGGCCTGACGTTAAAGGGCTTTCA
ACGCTTCAGGATATTGAAATAGGAGTGCAGCATATTTTAGCAGATATGATTGCTAAAGAC
AAAGACACGCTTGACTTCATTCGGAACTTGTGCCAGAAGAGACATGTTTGTATCCAGTCA
TCTCTGGCAAAAGTATCCTCAAAAAAGGTAAATGAGAAAGATGTTGATAAGTTTCTGCTC
TACCAGCATTTTTCCTGCAACATAAGAAACATTCACCATCATCAGATTCTGGCAATTAAC
CGTGGAGAAAATTTGAAGGTACTGACGGTTAAGGTCAATATTTCTGATGGAGTGAAGGAT
GAATTCTGTAGGTGGTGCATCCAAAACAGGTGGAGACCACGTAGCTTTGCAAGGCCAGAG
TTAATGAAGATCTTATATAATTCACTGAATGATTCCTTTAAACGCCTTATTTATCCTCTT
CTCTGTAGAGAATTCAGAGCCAAACTAACATCAGATGCAGAGAAGGAATCAGTAATGATG
TTTGGACGGAACCTTCGTCAGCTCCTTTTAACAAGCCCTGTTCCAGGGCGCACCTTAATG
GGAGTGGATCCTGGTTATAAACATGGTTGCAAATTAGCTATAATTTCTCCTACTAGTCAG
ATACTTCATACTGATGTGGTTTACTTGCATTGTGGACAAGGCTTCCGAGAGGCGGAGAAA
ATAAAGACACTTTTGCTGAATTTCAACTGCAGCACAGTAGTGATTGGAAATGGAACTGCC
TGCAGGGAAACAGAAGCTTACTTTGCTGACCTGATAATGAAGAATTATTTTGCACCACTG
GATGTTGTTTACTGTATCGTCAGTGAAGCAGGAGCATCAATCTACAGTGTCAGCCCTGAA
GCTAACAAAGAGATGCCAGGGCTGGACCCTAATTTGAGAAGTGCAGTTTCCATAGCAAGG
CGTGTACAAGATCCATTAGCTGAGCTAGTGAAAATTGAGCCAAAGCACATTGGAGTTGGA
ATGTATCAGCATGACGTATCCCAGACTTTACTCAAGGCAACACTGGACAGTGTTGTAGAA
GAATGTGTCAGCTTTGTGGGAGTGGATATTAACATCTGTTCAGAAGTTTTGTTAAGGCAT
ATTGCAGGACTCAATGCCAACAGGGCCAAAAATATTATTGAATGGCGAGAGAAAAATGGA
CCCTTTATCAACCGAGAACAGCTGAAGAAAGTGAAAGGGCTGGGCCCAAAATCCTTCCAA
CAGTGTGCTGGCTTCATCAGAATCAACCAGGATTATATCCGAACGTTTTGCAGTCAGCAA
ACTGAAACTTCAGGCCAAATTCAAGGAGTTGCTGTGACATCTTCAGCAGACGTTGAGGTC
ACAAATGAGAAGCAGGGCAAAAAGAAGAGCAAAACTGCAGTGAATGTTTTACTGAAGCCA
AATCCTTTGGACCAAACTTGTATTCATCCAGAATCATATGACATAGCAATGAGGTTTTTG
TCATCCATTGGAGGGACACTGTATGAGGTTGGAAAGCCTGAAATGCAACAAAAAATAAAT
TCATTCCTTGAAAAGGAAGGAATGGAGAAAATTGCAGAAAGATTGCAAACAACAGTACAC
ACCTTACAGGTCATCATAGATGGTCTCAGCCAGCCTGAAAGCTTTGACTTTCGAACAGAT
TTTGATAAACCTGATTTCAAGAGAAGCATAGTATGCCTGGAAGATCTGCAGATTGGGACA
GTTCTTACAGGCAAAGTTGAGAATGCCACTCTCTTTGGAATTTTTGTGGATATAGGAGTG
GGGAAATCTGGGCTGATTCCCATACGAAATGTAACAGAAGCAAAACTTTCAAAAACAAAG
AAGAGAAGAAGCCTTGGACTGGGCCCCGGAGAAAGAGTGGAAGTCCAAGTACTCAACATT
GACATCCCCCGATCTAGGATTACTCTGGACCTCATTCGGGTGTTA
Protein sequence predicted based on nucleotide sequence
MSSLPRRAKVQVQDVVLKDEFSSFSELSSASEEDDKEDSAWEPQKKVPRSRKQPPPKESK PKRMPRVKKNAPQISDGSEVVVVKEELNSSVAIADTALEDRKNKLDTVQTLKTAKTKQKC AAQPHTVRRTKKLKVEEETSKASNLEGESNSSETPSTSTVWGGTCKKEENDDDFTFGQSA LKKIKTETYPQGQPVKFPANANSTKEEVEMNWDMVQVLSERTNIEPWVCANIIRLFNDDN TIPFIIRYRKELINNLDADSLREVQQTLEELRAVAKKVHSTIQKIKKEGKMSECLLKAML NCKTFEELEHVSAPYKTGSKGTKAQRARQLGLEGAARALLEKPGELSLLSYIRPDVKGLS TLQDIEIGVQHILADMIAKDKDTLDFIRNLCQKRHVCIQSSLAKVSSKKVNEKDVDKFLL YQHFSCNIRNIHHHQILAINRGENLKVLTVKVNISDGVKDEFCRWCIQNRWRPRSFARPE LMKILYNSLNDSFKRLIYPLLCREFRAKLTSDAEKESVMMFGRNLRQLLLTSPVPGRTLM GVDPGYKHGCKLAIISPTSQILHTDVVYLHCGQGFREAEKIKTLLLNFNCSTVVIGNGTA CRETEAYFADLIMKNYFAPLDVVYCIVSEAGASIYSVSPEANKEMPGLDPNLRSAVSIAR RVQDPLAELVKIEPKHIGVGMYQHDVSQTLLKATLDSVVEECVSFVGVDINICSEVLLRH IAGLNANRAKNIIEWREKNGPFINREQLKKVKGLGPKSFQQCAGFIRINQDYIRTFCSQQ TETSGQIQGVAVTSSADVEVTNEKQGKKKSKTAVNVLLKPNPLDQTCIHPESYDIAMRFL SSIGGTLYEVGKPEMQQKINSFLEKEGMEKIAERLQTTVHTLQVIIDGLSQPESFDFRTD FDKPDFKRSIVCLEDLQIGTVLTGKVENATLFGIFVDIGVGKSGLIPIRNVTEAKLSKTK KRRSLGLGPGERVEVQVLNIDIPRSRITLDLIRVL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
