DNASU has two types of collections. The first are multi-plate collections of plasmids based on organism, vector or biological theme that you can request at a discounted price. The second are individual plasmids that are grouped together (such as the PSI or glyco-enzyme collections) so that you can find all plasmids from a particular biological collection more easily.
Thank you to all of the laboratories and individual investigators who have shared their plasmids!
Empty Vectors
| Transfer your gene of interest into these empty vectors for: |
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| Expression in a variety of systems including yeast, bacteria, mammalian |
| Search for plasmids based on vector features here |
Origins: PSI:Biology-Materials Repository Plasmids
| Explore 1000s of plasmids created by PSI researchers including: |
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| Over 106 empty vectors for expression and protein purification |
| Over 82,000 PSI-2 and PSI:Biology plasmids containing genes from over 500 organisms |
| Search for particular PSI plasmids using our PSI:Biology-MR Search |
Cystic Fibrosis Transmembrane Conductance Regulator Collection
| Explore over 350 CFTR plasmids: |
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| CFTR NBD1 and NBD2 domain constructs that have yielded protein structures in the PDB |
| Numerous mutations and truncations |
| Created by SGX Pharmaceuticals, Inc. (now part of Eli Lilly) |
Human Glyco-enzymes
| Explore over 1,300 human Glyco-enzymes: |
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| Including glycosyltransferases, glycosidases, sulfotransferases, and other glycan-related proteins |
| In both the Gateway entry vector and several expression vectors |
Cancer Expression Plasmids
| Explore over 200 plasmids targeting cancer related human proteins: |
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| Created by Argonne National Laboratory in collaboration with the NCI Clinical Proteomics Technology for Cancer (CPTC) Program |
Affinity Reagents
| Explore ~700 recombinant antibody (rAB) reagents: |
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| A collection of plasmids used to create renewable, high quality and high affinity recombinant antibodies generated by Recombinant Antibody Network as part of the NIH-funded Protein Capture Reagents Program |
Saccharomyces cerevisiae
| Explore ~16,000 sequence-verified ORFs: |
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| Genome-scale collection of ORFs in Gateway entry and two expression vectors (pBY011 and pZM552) |
Pseudomonas aeruginosa
| Explore over 2,300 sequence-verified plasmids: |
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| In the Gateway entry vector |
Bacillus anthracis
| Explore over 10,000 sequence-verified plasmids: |
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| In a Gateway entry vector |
| In a cell free expression vector |
Vibrio cholerae
| Genome-scale collection over 7,000 sequence-verified plasmids |
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| In a Gateway entry vector |
| In a GST-tagged cell free expression vector |
Francisella tularensis
| Genome-scale collection over 4,300 sequence-verified plasmids: |
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| In a Gateway entry vector |
| In an E. coli expression vector |
| In a yeast expression vector |
