The DNASU Plasmid Repository serves to reduce the burden on individual labs to store and maintain plasmid data and samples and to distribute these plasmids to researchers around the world. DNASU currently has a growing collection of over 200,000 plasmids with genes from more than 600 organisms that includes clones created in the LaBaer laboratory and various clones from outside researchers. We also have two prominate collaborations. As a founding member of the ORFeome Collaboration we have participated in creating thousands of human and mouse genes and are a key distirbutor for the ORFeome Collaboration plasmids and plasmid collections. Since 2006, as the PSI:Biology-Materials Repository, we have sequence verified and distributed tens of thousands of expression-ready clones created by researchers in the NIGMS-funded Protein Structure Initiative (PSI).
Each sample that enters our lab is sequence validated and annotation about each plasmid is stored in an Oracle-based database. Samples are stored in a state-of-the art Brooks BioStore Freezer Storage and Retrival system (funded by a NIGMS ARRA grant) that allows us to strack the plasmids and quickly retrieve requested plasmids. Plasmid information is searchable through this website and additional information about each plasmid can be found by clicking on the Clone ID or vector name. We have distributed over 200,000 plasmids to over 550 laboratories though our website, thus enabling researchers to execute the broadest possible variety of experiments on the functional roles for genes and proteins in biology and disease.
In addition to distributing materials, the MR has sought to simplify the MTA process in order to decrease the time it takes for institutions to deposit or receive plasmids. To achieve this goal, the MR pioneered two documents, the depositor's agreement, which sets forth the terms enabling the MR to distribute deposited plasmids from outside institutions, and the expedited process MTA, which eliminates the need for researchers to wait for their institutions to sign an MTA. In the future, the MR will continue to make PSI plasmids and data available to researchers and will expand its expedited MTA network so that researchers can receive PSI plasmids without delay.
PSI:Biology-Materials Repository (PSI:Biology-MR)
The Protein Structure Initiative's (PSI) main scientific mission is to make the three-dimensional atomic-level structures of most proteins easily obtainable from knowledge of their corresponding DNA sequences. Thus far over 5,100 protein structures have been determined by the 28 current and 3 former large scale structural genomics centers as part of the NIGMS-funded PSI project. The goals of the next phase of the PSI, PSI:Biology, which began the summer of 2010, are to continue solving protein structures and to apply high-throughput structural biology to important biological problems, which includes encouraging partnerships between structural biologists and investigators from the biological, biochemical, and/or molecular communities
In order to express, purify, crystallize and solve the structures of these protein families and biological targets, over 100,000 plasmid clones containing genes cloned from genomic DNA obtained from the host organism have been created. Many of these clones are of particular value to researchers because we have already tested and documented their ability to produce protein, including whether the protein was soluble and can be purified. All of this information is captured and searchable through DNASU. Additional biological and structural information about each of these clones can also be found by clicking on the CloneID and linking to the PSI Structural Biology Knowledgebase and the Protein Data Bank.
The Protein Structure Initiative-Materials Repository (PSI-MR), now the PSI:Biology-Materials Repository, was created in 2006 with the mission of storing, maintaining and distributing the over 100,000 clones created by all PSI researchers. In addition, each clone is sequence verified before made available for distribution, ensuring that the clone being sent to a researcher is correct. As of February 2014, over 82,000 PSI plasmids are available for distribution. For more information about he PSI:Biology-MR, visit the PSI:Biology-MR web portal or the PSI Structural Biology Knowledgebase.
Thank you for using the DNASU Plasmid repository as a resource for plasmids. As a non-profit entity based at a research institution, our ability to add and develop new clone collections to DNASU relies on grant funding. To track our resource's impact for future grant applications, we request that you cite DNASU in any papers using plasmids from the repository.
- Seiler, C.Y., Park, J.G., Sharma, A., Hunter, P., Surapaneni, P., Sedillo, C., Field, J., Alger, R., Steel, J., Throop, A., Fiacco, M., and LaBaer, J. (2013) DNASU plasmid and PSI:Biology-Materials repositories: resources to accelerate biological research. Nucleic Acids Research doi: 10.1093/nar/gkt1060 PMID: 24225319
- Cormier, C.Y., Park, J.G., Fiacco, M., Steel, J., Hunter, P., Kramer, J. Singla, R., Labaer, J. (2011). PSI:Biology-materials repository: a biologist's resource for protein expression plasmids. Journal of Structural and Functional Genomics. Jul;12(2):55-62. Epub 2011 Mar 1. PMID: 21360289
- Cormier, C.Y., Mohr, S.E., Zuo, D., Hu, Y., Rolfs, A., Kramer, J., Taycher, E., Kelley, F., Fiacco, M., Turnbull, G., and LaBaer, J. (2010). Protein Structure Initiative Material Repository: an open shared public resource of structural genomics plasmids for the biological community. Nucleic Acids Research 38(Database issue): D743-9. PMID: 19906724