TAF8 (S. cerevisiae )
In ZM552 (V5-tagged yeast expression vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH468536.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
ZM552
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
ScCD00101510
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1533nts
Open reading frame: 1 to 1533

ATGACTTCCAAAACGTCAGAAAGTGGTACCGGTACCCAAAGTACCATAGTTCAGCTGAGG
AACCTACCGGATTTGACGGAAATTAGTCACTTGGAAATAGATGCTCCCGTAGTTGAAATT
CTAAAGAAAACAGTACTTTTCCAGTTGAATTCGTTGAACATCTGCATTTCAAACTTTGCA
TTGGATGAGCTGGTCAACCTCGTGACCGTCCAAATGGACGGCATGTTTCGGAACCTGCAC
AATTTAACACTTTTACAAAGAAGGTCGCAAGCTTCTCAAGCAGATTTGAAATTGTTGCTT
CGAGAGTTTAACCTGGATGCGCCCTCCCTTTATCAGCAGTTTCAGGCCTCAGAATTCATC
AAATCGAAGCATTCCACGGAATATGAAAAATTGATGAGCTGGTCGTCTCTTGCTGCTTTA
CCGCATAACGAAGAAGATGAAGAGGACGAATTGAATAATATCGAGGAGCAACAGAACGAG
ATCAACGTTTTGCTGCCTCCCTCTAACCCACTGGAAAAACAGATACCGAGTTGGCTGCCC
AACTTTCCGCCTGACCATACCTATAAGTTTACACCTGAGTTCAACCATCCAATTACTGAT
CTAAAAACCATCAAGAAAGAAATAGTAAAAGAAAGCCAAGAGTCTGAAAAGGCACTATTG
AACTTGAACAAAAGTCTCTCCCACATATCATCAGCGTCAAATACCCCGCAACCGCCGGGT
TTAGACGATGAGGACGCTATCGAGCAACAATTGGAGATTTGGGGCAACGCGCTGGAAGAA
AGGAAACCAACCATAACTGAAAAGTCCTTCAATGAAAACAATATCGAGCAGTATGCGAAG
TATAGAGTGGAATTGGCGAGAGAACGTGTCACGAAATTTGAAGTGAATCAGTTGAAAAGA
ACAAAAAATCCATTCTTGAAAATTTCTGAAACTCTCTACCTTCCAGAGAGTCCTCACCAG
TCTCACAAAACCATACAAAAAACCATAGAATTGCAATTTCGTAAGTCTATGACACTATTC
ATGCACAATTTACCTAAGGTCCAGAAGTTGAAGAAGGAAAAAATACGAATGGCCAAGGAA
GAAAGAGCAAAATCGCTAAAACGTCGGCAAGAAGAGCTTATTTCACAACGAACAAAGAGG
GAACAAGATGAAGGCCATGATCTTGAGCTTCTACTAAATAACGAGCACGCAAGGGACGCT
GCAGACGACACGACCACCCCTAATGCATTAAACAACTCTACCATAGTTATCAACACAAAC
GCCGAGGATGAGGACGATGACATAAACTTATTTGGCATCTTAGGGAGCAGTGAAGATGAG
AATGAAATGTCGAGTATGCCTGCGGAGAACCTGGTCGCGGAATCGGAACCGCCAACAATG
ACAGCGCAAGATACTACAAATACTACACCTGTAGCACATAACACAACCAATATCGATGCT
ACTACAAGTCACTCTCCTCATTCGACGCCTAATGAAAACGCCCCGACATCTCCTCCTGCA
GATATAGCCACAGATCACGATATAACTATGTAG

Protein sequence predicted based on nucleotide sequence

MTSKTSESGTGTQSTIVQLRNLPDLTEISHLEIDAPVVEILKKTVLFQLNSLNICISNFA
LDELVNLVTVQMDGMFRNLHNLTLLQRRSQASQADLKLLLREFNLDAPSLYQQFQASEFI
KSKHSTEYEKLMSWSSLAALPHNEEDEEDELNNIEEQQNEINVLLPPSNPLEKQIPSWLP
NFPPDHTYKFTPEFNHPITDLKTIKKEIVKESQESEKALLNLNKSLSHISSASNTPQPPG
LDDEDAIEQQLEIWGNALEERKPTITEKSFNENNIEQYAKYRVELARERVTKFEVNQLKR
TKNPFLKISETLYLPESPHQSHKTIQKTIELQFRKSMTLFMHNLPKVQKLKKEKIRMAKE
ERAKSLKRRQEELISQRTKREQDEGHDLELLLNNEHARDAADDTTTPNALNNSTIVINTN
AEDEDDDINLFGILGSSEDENEMSSMPAENLVAESEPPTMTAQDTTNTTPVAHNTTNIDA
TTSHSPHSTPNENAPTSPPADIATDHDITM*

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 1533
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  yeast    Marker:  LEU2
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable