Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH464160.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
ZM552
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
ScCD00102552
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6156nts
Open reading frame: 1 to 6156
ATGGACGCTTACTCCACAAGACCATTAACCCTATCTCACGGTTCTTTAGAGCACGTGCTT
CTGGTACCAACCGCTTCATTTTTCATTGCTTCGCAATTACAAGAACAATTTAATAAAATT
TTGCCCGAACCCACTGAAGGGTTTGCTGCAGATGACGAGCCTACCACACCTGCTGAACTA
GTGGGGAAATTCCTTGGCTACGTATCTTCTCTAGTCGAACCTTCCAAGGTCGGTCAATTC
GATCAGGTCTTGAACCTTTGCTTAACAGAATTTGAAAACTGTTATTTAGAAGGCAATGAC
ATTCACGCCTTGGCTGCTAAACTATTACAGGAAAACGACACAACTTTAGTGAAGACTAAA
GAACTAATTAAAAATTATATTACCGCCAGAATAATGGCTAAGAGACCATTTGAGAAAAAA
TCCAACTCTGCTCTTTTTAGGGCCGTCGGCGAGGGTAACGCACAATTGGTAGCCATTTTC
GGTGGTCAAGGTAACACCGACGACTACTTTGAAGAATTGCGTGATCTATATCAAACTTAT
CATGTCTTAGTGGGAGATTTAATCAAGTTCTCCGCTGAAACTTTAAGTGAACTGATTAGA
ACTACTTTAGATGCTGAAAAAGTCTTTACTCAAGGTTTAAACATATTGGAATGGTTGGAG
AACCCTTCAAATACCCCAGACAAGGACTATTTACTTTCCATTCCAATTTCATGCCCCTTA
ATTGGTGTCATTCAATTGGCTCACTACGTAGTTACTGCCAAGCTTTTGGGTTTCACTCCA
GGTGAGTTAAGATCTTACTTAAAAGGTGCTACAGGTCACTCTCAAGGTTTGGTTACTGCT
GTCGCCATAGCTGAGACGGATTCCTGGGAATCCTTCTTCGTCTCCGTAAGAAAAGCAATT
ACTGTATTATTCTTCATCGGTGTTCGTTGTTACGAAGCATACCCAAACACTTCCCTACCA
CCATCCATCTTGGAAGATTCCTTGGAAAACAATGAAGGTGTTCCATCTCCAATGTTGTCC
ATTTCCAATCTAACTCAAGAACAAGTTCAAGACTATGTAAATAAGACTAACTCTCATTTG
CCAGCTGGTAAACAAGTTGAAATTTCTCTAGTCAATGGTGCGAAGAATCTAGTCGTATCG
GGCCCACCACAATCATTATATGGTTTAAACTTGACTTTAAGAAAGGCCAAGGCCCCATCT
GGACTGGATCAATCAAGAATCCCATTCAGCGAAAGAAAATTGAAGTTCTCCAATAGGTTC
TTACCTGTTGCATCACCATTCCATTCCCATCTATTGGTTCCAGCTTCAGATTTGATTAAC
AAAGACTTAGTCAAAAACAATGTCAGCTTTAACGCTAAAGATATTCAAATCCCCGTTTAC
GACACTTTTGATGGTTCAGATCTAAGAGTCCTTTCAGGTTCCATTTCCGAGAGAATCGTC
GACTGCATCATTAGATTACCTGTCAAATGGGAAACTACTACACAATTCAAAGCCACCCAC
ATATTAGACTTTGGTCCAGGTGGAGCTTCCGGTTTAGGTGTTTTAACCCATCGTAATAAA
GATGGTACTGGTGTTCGTGTTATCGTTGCCGGTACTCTCGACATTAACCCAGATGATGAT
TACGGATTCAAGCAAGAAATCTTTGATGTTACTAGTAATGGTTTGAAGAAAAATCCAAAC
TGGTTGGAAGAATACCATCCAAAATTAATTAAGAACAAATCAGGCAAAATTTTTGTCGAA
ACAAAATTTTCTAAATTAATCGGTAGACCACCTTTATTGGTTCCTGGTATGACACCATGT
ACTGTTTCTCCAGATTTCGTAGCTGCTACCACAAATGCTGGTTATACCATTGAGTTGGCC
GGTGGTGGTTACTTTTCCGCAGCAGGTATGACCGCCGCTATTGATTCTGTGGTTTCTCAG
ATAGAAAAGGGTAGTACCTTCGGTATCAACTTGATCTACGTCAATCCATTTATGTTACAA
TGGGGTATTCCATTAATCAAGGAACTAAGAAGCAAAGGTTATCCAATTCAATTCTTGACC
ATTGGTGCTGGTGTCCCATCATTGGAAGTTGCTAGTGAATACATAGAGACATTAGGTTTG
AAGTACTTGGGTTTGAAACCAGGTTCCATTGATGCTATTTCGCAAGTTATAAACATTGCT
AAAGCACATCCAAACTTCCCAATAGCTTTACAATGGACCGGTGGTAGAGGTGGTGGTCAT
CATTCTTTCGAAGATGCCCACACTCCAATGTTACAAATGTACTCCAAGATTAGAAGACAT
CCAAACATTATGTTGATATTCGGTTCTGGTTTCGGTTCTGCTGATGACACTTACCCATAC
TTAACCGGTGAATGGTCCACAAAATTCGATTATCCACCAATGCCATTCGATGGTTTCCTA
TTTGGTTCGAGGGTCATGATTGCTAAGGAAGTTAAAACTTCTCCTGATGCTAAGAAGTGT
ATTGCTGCTTGTACTGGTGTTCCTGATGATAAATGGGAACAAACCTACAAGAAGCCAACT
GGTGGTATTGTCACTGTTCGCTCTGAAATGGGTGAACCAATTCACAAAATTGCCACTCGT
GGTGTTATGCTATGGAAGGAATTCGACGAAACCATCTTCAACTTACCAAAGAATAAGTTG
GTACCAACTTTGGAAGCAAAGAGAGATTACATTATCTCAAGATTGAACGCCGATTTCCAA
AAACCATGGTTTGCTACCGTCAACGGTCAAGCCCGTGACCTAGCCACAATGACATACGAA
GAAGTTGCAAAGAGATTGGTGGAATTAATGTTCATCAGATCTACCAACTCTTGGTTTGAT
GTCACATGGAGAACCTTTACTGGTGATTTCCTACGTCGTGTCGAAGAACGTTTCACTAAA
AGTAAGACATTGTCTTTAATCCAATCCTATTCTCTACTAGACAAGCCTGATGAAGCTATT
GAAAAAGTATTTAATGCTTATCCTGCCGCTAGGGAACAGTTCTTGAATGCGCAAGATATT
GATCACTTTTTGAGCATGTGTCAAAATCCAATGCAAAAACCAGTGCCTTTTGTTCCAGTT
TTGGATCGTAGATTCGAGATTTTTTTCAAAAAAGATTCGTTATGGCAATCTGAGCACTTG
GAAGCCGTCGTCGACCAAGACGTTCAAAGAACATGTATCCTACATGGACCTGTTGCAGCA
CAATTCACTAAAGTCATCGATGAACCAATTAAGAGCATTATGGATGGTATTCACGATGGT
CACATCAAAAAGTTACTACATCAATATTACGGTGACGATGAGTCAAAGATTCCAGCAGTT
GAGTACTTTGGTGGTGAAAGCCCTGTAGACGTACAAAGTCAAGTTGATTCTTCCTCTGTA
TCTGAAGACTCAGCTGTTTTTAAGGCAACATCCTCTACTGATGAAGAAAGCTGGTTTAAG
GCTTTGGCGGGATCCGAAATTAACTGGAGACATGCAAGTTTCTTATGTTCCTTTATCACT
CAAGATAAAATGTTTGTTTCTAACCCAATTAGAAAAGTTTTCAAGCCAAGCCAAGGAATG
GTTGTTGAGATTTCCAACGGCAATACTTCTTCAAAGACTGTTGTCACTCTTTCAGAACCT
GTTCAAGGTGAATTGAAACCAACTGTTATTTTGAAGTTGTTGAAGGAGAACATAATCCAA
ATGGAAATGATTGAGAACAGAACTATGGATGGTAAGCCCGTCAGCTTGCCATTGTTGTAC
AACTTCAACCCAGATAATGGTTTTGCTCCAATCTCTGAAGTTATGGAGGACAGAAACCAA
AGAATTAAGGAAATGTACTGGAAATTATGGATTGATGAGCCTTTCAATTTGGACTTTGAC
CCAAGAGATGTCATTAAGGGCAAAGATTTCGAGATCACCGCTAAAGAAGTTTATGACTTT
ACACACGCTGTTGGAAACAATTGTGAAGACTTCGTTTCTAGACCTGATAGAACGATGTTG
GCCCCAATGGACTTTGCTATTGTTGTCGGATGGAGAGCCATCATCAAGGCCATTTTCCCT
AATACGGTCGATGGTGACTTATTGAAGTTGGTTCATTTGTCTAACGGCTACAAGATGATT
CCTGGCGCTAAGCCACTGCAAGTTGGTGATGTTGTTTCAACTACTGCTGTTATTGAATCT
GTCGTCAACCAACCTACAGGAAAGATTGTCGATGTGGTAGGTACATTATCGAGAAATGGC
AAGCCTGTCATGGAAGTCACCTCCTCATTCTTCTACAGAGGCAACTATACTGACTTTGAA
AACACTTTCCAAAAGACTGTTGAACCTGTTTATCAAATGCACATCAAAACTTCTAAAGAT
ATAGCTGTCTTGCGCTCTAAGGAGTGGTTCCAATTGGACGATGAAGACTTCGATCTGTTA
AACAAAACTTTGACTTTCGAAACTGAAACTGAAGTTACTTTCAAGAATGCTAACATCTTC
TCTTCAGTGAAATGTTTTGGCCCAATTAAAGTTGAATTGCCAACCAAAGAAACCGTGGAG
ATCGGTATTGTCGATTACGAAGCCGGTGCCTCTCACGGTAACCCTGTTGTTGATTTCTTG
AAGAGAAACGGTTCCACATTGGAACAAAAGGTCAATCTAGAAAATCCTATTCCAATTGCA
GTACTTGATTCGTACACTCCAAGTACCAACGAACCATACGCTAGAGTTTCTGGTGATTTG
AATCCAATTCACGTTTCACGTCATTTTGCCTCTTACGCAAACTTGCCAGGTACTATCAAG
CACGGTATGTTTTCTTCTGCTTCCGTCCGTGCTTTGATTGAAAACTGGGCTGCTGACAGT
GTTTCATCCAGGGTACGTGGCTACACTTGTCAATTTGTTGACATGGTTTTGCCTAACACT
GCTTTGAAAACATCGATTCAACATGTTGGTATGATCAATGGTAGAAAATTGATAAAGTTT
GAAACTAGAAATGAAGATGACGTTGTAGTTTTGACTGGTGAAGCCGAAATTGAACAACCT
GTTACTACCTTCGTTTTCACTGGTCAAGGTTCACAAGAACAAGGTATGGGTATGGACTTA
TACAAAACTTCTAAAGCTGCTCAAGATGTTTGGAATAGAGCTGACAACCATTTCAAGGAC
ACTTATGGTTTCTCTATCTTAGACATTGTCATTAACAACCCAGTTAACTTAACAATTCAC
TTCGGTGGTGAAAAGGGTAAGAGGATCAGAGAAAACTATTCTGCTATGATCTTTGAGACT
ATCGTGGATGGAAAATTGAAGACTGAAAAAATTTTCAAGGAAATTAATGAGCACAGTACT
TCTTACACATTTAGATCTGAAAAAGGTTTATTGTCTGCTACTCAATTTACACAACCAGCT
TTAACTTTGATGGAAAAAGCTGCTTTCGAAGACTTGAAATCTAAAGGTTTGATCCCAGCC
GATGCTACTTTTGCTGGTCACTCTTTAGGTGAGTATGCTGCTTTGGCCTCTTTGGCTGAT
GTTATGTCTATCGAATCTTTAGTTGAAGTTGTGTTCTACAGAGGTATGACTATGCAAGTT
GCTGTTCCAAGAGATGAGTTGGGCAGATCCAACTATGGTATGATTGCCATTAACCCAGGT
AGAGTCGCTGCATCATTCTCTCAAGAAGCTTTGCAATATGTTGTTGAGAGAGTTGGTAAG
AGAACCGGCTGGTTGGTTGAAATCGTCAACTACAACGTTGAAAACCAACAATATGTTGCA
GCTGGTGATCTAAGAGCTTTAGACACCGTTACCAATGTTCTAAACTTCATCAAATTACAA
AAAATTGATATTATTGAACTACAAAAGTCCTTATCTTTGGAAGAAGTTGAAGGTCATTTG
TTTGAGATCATTGACGAAGCTTCCAAGAAATCTGCTGTCAAGCCTCGCCCACTTAAATTG
GAGAGAGGTTTTGCTTGTATCCCATTAGTTGGTATTTCTGTTCCTTTCCATTCCACCTAC
TTGATGAATGGTGTTAAACCATTCAAGAGTTTCTTGAAGAAGAATATCATAAAAGAAAAT
GTGAAGGTTGCTAGATTGGCCGGAAAGTACATTCCAAACTTGACTGCAAAACCATTCCAG
GTTACTAAGGAATATTTCCAGGACGTTTATGATTTGACTGGCTCCGAACCTATCAAGGAA
ATCATCGACAACTGGGAAAAGTATGAACAATCCTAG
Protein sequence predicted based on nucleotide sequence
MDAYSTRPLTLSHGSLEHVLLVPTASFFIASQLQEQFNKILPEPTEGFAADDEPTTPAEL VGKFLGYVSSLVEPSKVGQFDQVLNLCLTEFENCYLEGNDIHALAAKLLQENDTTLVKTK ELIKNYITARIMAKRPFEKKSNSALFRAVGEGNAQLVAIFGGQGNTDDYFEELRDLYQTY HVLVGDLIKFSAETLSELIRTTLDAEKVFTQGLNILEWLENPSNTPDKDYLLSIPISCPL IGVIQLAHYVVTAKLLGFTPGELRSYLKGATGHSQGLVTAVAIAETDSWESFFVSVRKAI TVLFFIGVRCYEAYPNTSLPPSILEDSLENNEGVPSPMLSISNLTQEQVQDYVNKTNSHL PAGKQVEISLVNGAKNLVVSGPPQSLYGLNLTLRKAKAPSGLDQSRIPFSERKLKFSNRF LPVASPFHSHLLVPASDLINKDLVKNNVSFNAKDIQIPVYDTFDGSDLRVLSGSISERIV DCIIRLPVKWETTTQFKATHILDFGPGGASGLGVLTHRNKDGTGVRVIVAGTLDINPDDD YGFKQEIFDVTSNGLKKNPNWLEEYHPKLIKNKSGKIFVETKFSKLIGRPPLLVPGMTPC TVSPDFVAATTNAGYTIELAGGGYFSAAGMTAAIDSVVSQIEKGSTFGINLIYVNPFMLQ WGIPLIKELRSKGYPIQFLTIGAGVPSLEVASEYIETLGLKYLGLKPGSIDAISQVINIA KAHPNFPIALQWTGGRGGGHHSFEDAHTPMLQMYSKIRRHPNIMLIFGSGFGSADDTYPY LTGEWSTKFDYPPMPFDGFLFGSRVMIAKEVKTSPDAKKCIAACTGVPDDKWEQTYKKPT GGIVTVRSEMGEPIHKIATRGVMLWKEFDETIFNLPKNKLVPTLEAKRDYIISRLNADFQ KPWFATVNGQARDLATMTYEEVAKRLVELMFIRSTNSWFDVTWRTFTGDFLRRVEERFTK SKTLSLIQSYSLLDKPDEAIEKVFNAYPAAREQFLNAQDIDHFLSMCQNPMQKPVPFVPV LDRRFEIFFKKDSLWQSEHLEAVVDQDVQRTCILHGPVAAQFTKVIDEPIKSIMDGIHDG HIKKLLHQYYGDDESKIPAVEYFGGESPVDVQSQVDSSSVSEDSAVFKATSSTDEESWFK ALAGSEINWRHASFLCSFITQDKMFVSNPIRKVFKPSQGMVVEISNGNTSSKTVVTLSEP VQGELKPTVILKLLKENIIQMEMIENRTMDGKPVSLPLLYNFNPDNGFAPISEVMEDRNQ RIKEMYWKLWIDEPFNLDFDPRDVIKGKDFEITAKEVYDFTHAVGNNCEDFVSRPDRTML APMDFAIVVGWRAIIKAIFPNTVDGDLLKLVHLSNGYKMIPGAKPLQVGDVVSTTAVIES VVNQPTGKIVDVVGTLSRNGKPVMEVTSSFFYRGNYTDFENTFQKTVEPVYQMHIKTSKD IAVLRSKEWFQLDDEDFDLLNKTLTFETETEVTFKNANIFSSVKCFGPIKVELPTKETVE IGIVDYEAGASHGNPVVDFLKRNGSTLEQKVNLENPIPIAVLDSYTPSTNEPYARVSGDL NPIHVSRHFASYANLPGTIKHGMFSSASVRALIENWAADSVSSRVRGYTCQFVDMVLPNT ALKTSIQHVGMINGRKLIKFETRNEDDVVVLTGEAEIEQPVTTFVFTGQGSQEQGMGMDL YKTSKAAQDVWNRADNHFKDTYGFSILDIVINNPVNLTIHFGGEKGKRIRENYSAMIFET IVDGKLKTEKIFKEINEHSTSYTFRSEKGLLSATQFTQPALTLMEKAAFEDLKSKGLIPA DATFAGHSLGEYAALASLADVMSIESLVEVVFYRGMTMQVAVPRDELGRSNYGMIAINPG RVAASFSQEALQYVVERVGKRTGWLVEIVNYNVENQQYVAAGDLRALDTVTNVLNFIKLQ KIDIIELQKSLSLEEVEGHLFEIIDEASKKSAVKPRPLKLERGFACIPLVGISVPFHSTY LMNGVKPFKSFLKKNIIKENVKVARLAGKYIPNLTAKPFQVTKEYFQDVYDLTGSEPIKE IIDNWEKYEQS*
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
6156
Mutations:
None
Discrepancy:
c414g,D138E; c4739a,T1580K
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
yeast
Marker:
LEU2
Host Type: bacterial Marker: ampicillin
Host Type: bacterial Marker: ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
ZM552
Synonyms:
ZM552
Description:
Yeast expression vector with N-terminal 3X V5 epitope tag; ampicillin resistance in bacteria; LEU2 resistance in yeast; Gateway cloning.
Comments:
ZM511 Stu1 vector plus Gateway attR frame B cassette EcoRV fragment; same as ZM515. Cloning into the vector removes the ccdB cassette and chloramphenicol resistance.
Size (bp):
8302
Parent Vector:
None
Properties:
Gateway, acceptor (destination), recombinational cloning, with tag/fusion/marker, yeast expression
Author Name:
Zarmik Moqtaderi
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
primer site
M13F
M13 forward sequencing primer
8286
8301
primer site
M13R
M13 reverse sequencing primer
2630
2647
promoter
CMD1
CMD1 promoter
295
484
recombination site
attR1
attR1 recombinational cloning site
650
666
recombination site
attR2
attR2 recombination site
2337
2352
reporter gene
LacZ
LacZ alpha gene
8146
8214
selectable marker
AmpR
ampcillin resistance
3795
4454
selectable marker
CmR
chloramphenicol resistance. removed with the ccdB cassette in clones with insert
883
1539
selectable marker
LEU2
LEU2 selectable marker in yeast
5273
5344
tag
V5
N-terminal triple V5 tag
494
631
trxn termination sequence
CYC1
CYC1 terminator
2377
2582
yeast ARS
ARS
ARS yeast origin of replication
0
0
yeast centromere
CEN
yeast centromere
0
0
