Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH469054.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
ZM552
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
ScCD00103085
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3657nts
Open reading frame: 1 to 3657
ATGATAAAAAATGGTACATGCCCCTATTGGGAACGTGATGATCTTTCGGAATGTGCTAGG
CGAGAATATATCGAGTTTAAATTTCCTCTATTTATATTGTTGACTGGTATGATATACGCG
TTTTGCAAAGTCTTTCGAGCCTTTTATCTAAGGGGGAAAAATCATACAAATGAAGCGCCA
GAATTTGAAGAACAAGGTAATGGAAACCACGAATATGCAAGGTTCTCAGTTTTAAGACTA
AAATCTGCATGGGAAAGCCGTAGCTTTTGTAATGTTAATAATAGATCTACTTTCGACAAA
TTCAAAAAATTTATAGAGGGTGCCTTCATTGTTTTGCAGCTTACTATCCACCTGTATATT
TTATCTAGTATGCCAATGGATAACAAAAAGTTCTTTCACCAAGGTTTTCTGGTTCAAATG
TTTCTCTGGATTTTACTGCTTGTTGTTATTACACTTCGTTTAATTTCAGCAAGCCAGTCA
TTTCGGTGGGTTTTAGCATGTAAGCGTGATTTATGGGCTGTTTCGTTTTACTCATATGCA
TCCCTGTTTACTCTTTCGATTTTACCTCTTCGTTCCGTCTTCATTGGAAAGATAAAAGAT
AAAATTATGGTCAAATATATCATTTCCGAGACTTTCATTGATTTAGCGCTTTTATTATTA
TTATCAACGTCAAGTATAGAAGGAACTAGGTACTCATTTTTGGTTGAAAATGAGAACAAA
AAGTTGCCACCGGCTCCTACCGTCTTTGGCCTTCTTACTTTTTCTAGGATTGATCGGCTT
ATTTGGAAAGCATACAAACACTGCCTTGGAAACGCTGATATTTGGGATTTGGATATTAAT
AATAAGTCTATAGCAATTTTGGCGAACTTTGAAATGTCTTCTAAAAAAGGAAGGCTCCTT
CCAAACATTATTTGTTATTTTAAGGCTGTTTTCATCTCCCAATTATTTCTGGCTTTTGTA
TCTAGTTTTTTGAACTTTGTACCCTCATTGTTGATGCCAAGAATACTATCATACGTTAAT
GACCCAAAGTCAAAATCATGGAACTTGGTGTCTTTATACGTCTCATCTATGCTCGTCAGT
AAAATCATTGCCACGACTTGTAGAGGTCAAGGATTGTTTTTAGGTGAAAAGGGTACTATG
CAACTAAGAACAGTTTTGATATCCAATATCTATTCCAAAACCTTAAGAAGAACAATTCTA
AAAGACTCAACAACGTCACTTCAAAAAAATGCGTCAACATCCTTTGAAGAAAATCCTGAC
TCTTCTGAAGCGGAACCTAGAAAAAAGTCTAGTAGGAAAGACAACTCTGTGAACAATGTT
ATGTCAATTGACGCTTTCAAGGTTTCTGAAGCTATGAACACTTTTTATCTGGCATGTGAA
GCAGTTTTCATGACAGTTACGGCCCTAATGATACTATATTCCCTACTGGGATGGTCCGCC
TTTGCTGGTACCTTTGCTCTTCTTGCCATGATTCCTTTGAATTTTTGGTGTGCAACTTTT
TACGGGAACTATCAGGCCGATCAATTAATCCTAACTGACAAGCGTACCTCTGGGATTAGT
GAGGCTCTGAACTCAATACGTGTAATAAAGCTACTGGCATGGGAAAATCTGTTTTATCAA
AAGATTATAAACGTAAGAGATGGGGAAATAAGACTCCTTAAAAAGAAGGCAACAATTTTC
TTTTTGAACCATCTCATTTGGTTCTTTGGGCCCACTTTGGTCTCTGCAATAACATTCTCA
GTGTTTATTAAATTCCAAAATCAGACACTTACTCCTACAATAGCATTTACAGCTCTTTCT
TTATTTGCAATATTGAGGACACCCATGGACCAAATTGCTTCGACTGTCAGCCTTTTGATA
CAGTCTTTTATTTCTCTTGAAAGAATCCAGGATTATCTTAATGAATCAGAAACAAGAAAA
TATGAGATTTTGGAACAAAGCAATACTAAATTTGGCTTTGAAGATGCAAGCATGGAATGG
GAAGCTGCTGAAACAAGTTTTAAACTTAAGAACATTTCTATTGATTTTAAGCTGAATAGT
CTCAACGCAATTATAGGTCCGACTGGGTCAGGAAAGTCTTCGCTATTACTTGGACTATTG
GGAGAATTGAACCTTCTTTCTGGAAAAATATACGTACCTACAGTTGAATCCCGCGACGAC
TTAGAGATTGGTAAAGACGGAATGACGAATTCAATGGCATATTGTTCTCAAACTCCGTGG
TTGATTAGTGGAACAATTAAAGATAACGTTGTTTTTGGAGAAATCTTCAACAAACAAAAA
TTTGATGATGTAATGAAATCCTGTTGTCTTGACAAAGATATCAAAGCAATGACAGCTGGC
ATAAGAACAGACGTGGGTGATGGAGGATTTTCCTTATCTGGCGGACAGCAGCAAAGGATT
GCTTTAGCCAGAGCAATTTACTCCTCTTCCAGGTATTTGATCCTTGATGATTGCTTGAGT
GCAGTAGATCCTGAAACTGCACTTTATATATATGAAGAGTGTTTATGCGGCCCCATGATG
AAAGGAAGGACCTGCATCATTACGAGTCATAATATTTCTTTAGTTACGAAACGGGCTGAC
TGGCTTGTGATTTTAGATCGTGGCGAGGTGAAATCACAGGGTAAACCATCGGACCTCATT
AAATCTAATGAGTTTTTGAGGGAAAGCATAAACAATGATTCAAAAAATACAACTCACAAT
CAAATTGACTTGAAAAGATCAACAACATCAAAGAAAACTAAGAATGGAGATCCCGAGGGA
GGGAACTCGCAAGACGAAGTGTGTGCTGAAGTTGAAAATTTTGAGGAAACAAAAATGGAA
GGATCAGTTAAATTTTCAGCTTACAAATGGTTAGCAGACTATTTCGGGGGACTGGGAGTT
GTTTTTGTTTTCACCTCGTCCTCCATCCTTATTCATGGAATCACACTGTCTCAGGGCTTT
TGGCTCAGATACTGGCTGGACACTGGATCTTCAGGGAGTAAGTCTACCTGGCTTTATAGA
ATAGTTGAGGGTCACTCTAACATCTATTTCTTACTGACTTATATTATCATAGGTCTTGTT
TCTTCATTTTTAACTTCTGGTAAAGTTTGGATAGCAATAATTTCAGGTACCAACGTCACC
AAGAAAATATTTGCGAAGCTTCTATCCAGTATCTTATATGCCAAGTTACGTTTTCATAAT
GTCACGCCGACTGGAAGAATAATGAACAGATTTAGCAAGGATATGGATATTATTGATCAA
CAATTGATCCCTAATTTCGAAGGACTCTCTTATAGCGTCGTTGTTTGTCTGTGGATTATA
CTTTTAATTGGATATGTTACTCCTCAATTTCTGTTATTTGCTATTCCTTTATGCGCTCTT
TATTATACCGTATGTACGTTATATCTTCGTGCATCTAGAGAGTTGAAGAGAATAGATAAC
ATCAATATCTCTCCAATACACCAGTTATTCGCTGAAGCCATCAAAGGAGTAACTACAATT
AGAGCATTAGCAGATGAGCGTAGGTTTATCACTCAATCTTTGGTTGCAATTGACAGAAGT
AATGCTCCATTTTTTTATCTCAATATGGCCACCGAGTGGATCACATATAGAGTGGATATA
ATTGGGACACTTGTTCTTTTTAGTTCTTCTGTAATGATCATAATGAAGGCCTCATAG
Protein sequence predicted based on nucleotide sequence
MIKNGTCPYWERDDLSECARREYIEFKFPLFILLTGMIYAFCKVFRAFYLRGKNHTNEAP EFEEQGNGNHEYARFSVLRLKSAWESRSFCNVNNRSTFDKFKKFIEGAFIVLQLTIHLYI LSSMPMDNKKFFHQGFLVQMFLWILLLVVITLRLISASQSFRWVLACKRDLWAVSFYSYA SLFTLSILPLRSVFIGKIKDKIMVKYIISETFIDLALLLLLSTSSIEGTRYSFLVENENK KLPPAPTVFGLLTFSRIDRLIWKAYKHCLGNADIWDLDINNKSIAILANFEMSSKKGRLL PNIICYFKAVFISQLFLAFVSSFLNFVPSLLMPRILSYVNDPKSKSWNLVSLYVSSMLVS KIIATTCRGQGLFLGEKGTMQLRTVLISNIYSKTLRRTILKDSTTSLQKNASTSFEENPD SSEAEPRKKSSRKDNSVNNVMSIDAFKVSEAMNTFYLACEAVFMTVTALMILYSLLGWSA FAGTFALLAMIPLNFWCATFYGNYQADQLILTDKRTSGISEALNSIRVIKLLAWENLFYQ KIINVRDGEIRLLKKKATIFFLNHLIWFFGPTLVSAITFSVFIKFQNQTLTPTIAFTALS LFAILRTPMDQIASTVSLLIQSFISLERIQDYLNESETRKYEILEQSNTKFGFEDASMEW EAAETSFKLKNISIDFKLNSLNAIIGPTGSGKSSLLLGLLGELNLLSGKIYVPTVESRDD LEIGKDGMTNSMAYCSQTPWLISGTIKDNVVFGEIFNKQKFDDVMKSCCLDKDIKAMTAG IRTDVGDGGFSLSGGQQQRIALARAIYSSSRYLILDDCLSAVDPETALYIYEECLCGPMM KGRTCIITSHNISLVTKRADWLVILDRGEVKSQGKPSDLIKSNEFLRESINNDSKNTTHN QIDLKRSTTSKKTKNGDPEGGNSQDEVCAEVENFEETKMEGSVKFSAYKWLADYFGGLGV VFVFTSSSILIHGITLSQGFWLRYWLDTGSSGSKSTWLYRIVEGHSNIYFLLTYIIIGLV SSFLTSGKVWIAIISGTNVTKKIFAKLLSSILYAKLRFHNVTPTGRIMNRFSKDMDIIDQ QLIPNFEGLSYSVVVCLWIILLIGYVTPQFLLFAIPLCALYYTVCTLYLRASRELKRIDN INISPIHQLFAEAIKGVTTIRALADERRFITQSLVAIDRSNAPFFYLNMATEWITYRVDI IGTLVLFSSSVMIIMKAS*
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
3657
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
yeast
Marker:
LEU2
Host Type: bacterial Marker: ampicillin
Host Type: bacterial Marker: ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
ZM552
Synonyms:
ZM552
Description:
Yeast expression vector with N-terminal 3X V5 epitope tag; ampicillin resistance in bacteria; LEU2 resistance in yeast; Gateway cloning.
Comments:
ZM511 Stu1 vector plus Gateway attR frame B cassette EcoRV fragment; same as ZM515. Cloning into the vector removes the ccdB cassette and chloramphenicol resistance.
Size (bp):
8302
Parent Vector:
None
Properties:
Gateway, acceptor (destination), recombinational cloning, with tag/fusion/marker, yeast expression
Author Name:
Zarmik Moqtaderi
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
primer site
M13F
M13 forward sequencing primer
8286
8301
primer site
M13R
M13 reverse sequencing primer
2630
2647
promoter
CMD1
CMD1 promoter
295
484
recombination site
attR1
attR1 recombinational cloning site
650
666
recombination site
attR2
attR2 recombination site
2337
2352
reporter gene
LacZ
LacZ alpha gene
8146
8214
selectable marker
AmpR
ampcillin resistance
3795
4454
selectable marker
CmR
chloramphenicol resistance. removed with the ccdB cassette in clones with insert
883
1539
selectable marker
LEU2
LEU2 selectable marker in yeast
5273
5344
tag
V5
N-terminal triple V5 tag
494
631
trxn termination sequence
CYC1
CYC1 terminator
2377
2582
yeast ARS
ARS
ARS yeast origin of replication
0
0
yeast centromere
CEN
yeast centromere
0
0
