Title: Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation.
Special MTA: None
ATGTCTGCTTGTAACACCTTTACTGAACATGTTTGGAAACCTGGTGAATGCAAGAATTGC
TTTAAACCTAAAAGTTTGCACCAGCTTCCCCCAGACCCTGAGAAGGCACCCATCACCCAT
GGCAATGTGAAAACTAATGCCAATCACAGTAACAACCACCGCATCAGGAACACGGGCAAT
TTCCGGCCTCCTGTGGCTAAAAAACCCACTATAGCTGTGAAGCCCACTATGATAGTGGCA
GATGGGCAAAGTATATGTGGTGAGCTTAGCATCCAAGAACACTGTGAGAACAAACCTGTC
ATCATAGGGTGGAACCGAAACAGAGCTGCCTTGAGTCAGAAACCACTTAACAATAATAAT
GAAGATGATGAAGGAATTAGCCATGTTCCTAAGCCTTATGGCAATAATGATAGTGCAAAG
AAGATGTCAGATAACAATAATGGACTAACTGAAGTGTTAAAGGAGATAGCAGGCTTGGAT
ACTGCCCCTCAGATAAGAGGAAATGAAACAAACTCCAGAGAAACATTCTTGGGAAGAATA
AATGATTGCTATAAACGATCATTGGAAAGAAAGCTTCCACCAAGTTGCATGATAGGTGGG
ATAAAGGAAACTCAGGGCAAGCATGTTATTCTGAGTGGGAGCACAGAAGTGATTAGTAAT
GAAGGGGGCCGGTTCTGTTACCCAGAGTTTTCCAGTGGCGAGGAGAGTGAAGAGGATGTA
CTTTTCAGTAACATGGAGGAGGAGCACGAGAGTTGGGATGAGAGTGATGAAGAGCTGTTG
GCCATGGAGATTCGCATGAGAGGGCAACCTCGCTTTGCCAACTTCAGAGCAAACACATTG
TCTCCTGTTCGATTCTTTGTGGACAAAAAATGGAATACCATCCCCCTGCGAAACAAGTCT
CTGCAGAGAATCTGTGCTGTGGACTATGATGACAGCTATGATGAAATCCTGAATGGTTAT
GAGGAAAATTCTGTGGTCTCTTATGGACAAGGAAGCATTCAGAGCATGGTGTCATCTGAC
TCCACATCACCAGATTCTTCTTTAACAGAAGAATCACGTTCTGAGACAGCCAGTAGTTTA
TCCCAGAAGATTTGTAATGGGGGATTATCTCCTGGTAACCCAGGAGATTCTAAGGACATG
AAGGAAATTGAGCCCAATTATGAAAGTCCCTCTAGTAATAATCAGGATAAAGATTCATCA
CAGGCTTCCAAAAGCTCAATAAAAGTTCCAGAGACCCACAAAGCAGTCCTTGCTCTCCGA
TTAGAAGAGAAAGATGGCAAGATTGCTGTACAAACTGAGAAGGAAGAAAGTAAAGCCTCT
ACAGATGTTGCTGGGCAAGCAGTAACCATAAACCTTGTCCCCACAGAAGAGCAAGCAAAA
CCTTACCGAGTTGTGAACCTGGAACAGCCATTGTGCAAGCCATATACTGTCGTGGATGTG
TCAGCAGCCATGGCCAGTGAGCACCTCGAGGGCCCTGTTAACAGCCCCAAGACAAAAAGC
TCATCCTCTACTCCAAACTCTCCAGTTACATCATCTTCATTGACACCAGGACAAATAAGT
GCCCATTTCCAAAAATCCAGTGCAATTCGATACCAAGAAGTATGGACTTCTAGCACCAGT
CCACGACAAAAGATACCTAAAGTAGAACTAATTACTAGTGGAACTGGACCAAATGTTCCT
CCAAGGAAAAACTGTCACAAATCAGCACCTACATCACCCACAGCTACAAACATTTCCTCC
AAAACCATCCCTGTTAAGTCACCTAATTTGTCTGAAATTAAATTTAATAGTTATAACAAT
GCTGGTATGCCACCTTTTCCAATTATCATTCATGACGAGCCAACTTATGCTCGGAGTTCC
AAAAATGCTATCAAAGTTCCCATTGTTATCAATCCAAATGCATATGACAATCTAGCTATC
TACAAAAGTTTTCTGGGAACAAGTGGAGAACTCTCAGTGAAGGAAAAAACCACAAGTGTA
ATAAGCCATACTTATGAAGAAATAGAAACAGAAAGCAAAGTGCCTGATAACACCACTAGC
AAAACCACTGACTGTCTTCAAACTAAAGGGTTTTCAAACAGCACAGAGCATAAAAGGGGC
TCAGTGGCTCAGAAGGTTCAAGAGTTTAACAACTGTCTCAACAGAGGTCAGTCTTCACCA
CAGAGAAGCTATAGTTCCAGCCACAGCTCCCCAGCAAAGATCCAGAGAGCCACTCAAGAG
CCTGTGGCCAAAATAGAAGGCACTCAGGAGTCTCAGATGGTGGGCAGCAGCAGCACCAGA
GAGAAAGCAAGCACAGTGCTTTCTCAGATTGTGGCTTCAATCCAACCCCCACAGTCTCCT
CCAGAAACACCTCAATCTGGCCCTAAAGCTTGCAGTGTGGAAGAGCTTTATGCCATTCCT
CCAGATGCTGATGTTGCTAAGAGCACACCTAAGAGTACGCCAGTCCGGCCCAAATCTCTC
TTTACATCTCAGCCTAGTGGTGAGGCTGAAGCACCTCAGACCACAGACAGTCCTACCACC
AAAGTACAGAAAGACCCATCCATAAAGCCAGTCACCCCCTCTCCCTCCAAATTAGTGACT
AGCCCCCAAAGTGAGCCACCAGCTCCCTTTCCCCCGCCACGCTCTACTTCTTCTCCTTAC
CATGCAGGTAACCTTTTGCAGAGGCATTTCACCAACTGGACCAAGCCAACCAGCCCTACC
AGGTCAACAGAAGCTGAATCAGTTTTGCACTCTGAAGGCAGCAGGCGGGCAGCTGATGCA
AAACCTAAGCGCTGGATATCATTTAAAAGCTTCTTCCGCCGTCGGAAAACAGATGAGGAG
GATGACAAAGAGAAAGAGCGAGAGAAAGGGAAACTGGTGGGCCTGGATGGCACAGTCATT
CACATGCTGCCTCCTCCTCCAGTTCAGCGCCATCACTGGTTCACAGAGGCGAAAGGAGAG
TCCAGTGAGAAACCAGCCATTGTCTTCATGTACAGGTGCGACCCTGCTCAAGGCCAGCTC
AGTGTGGATCAGAGCAAGGCTAGGACAGACCAGGCAGCAGTCATGGAGAAGGGTAGAGCA
GAGAATGCATTACTACAGGACTCAGAGAAGAAGAGGAGTCATTCTTCTCCATCACAGATT
CCTAAAAAGATTCTCAGTCACATGACCCATGAAGTAACAGAGGATTTTTCTCCTCGGGAT
CCAAGAACTGTTGTTGGGAAGCAAGATGGCAGGGGCTGCACTTTAGTCACAACAGCATTG
TCCCTACCTGAACTGGAAAGGGAAGATGGAAAAGAAGACATTTCAGATCCTATGGACCCG
AACCCTTGTAGTGCAACATACAGCAACTTAGGGCAATCTAGAGCAGCCATGATACCTCCC
AAGCAGCCACGACAGCCCAAGGGAGCTGTGGACGATGCCATCGCCTTTGGAGGGAAAACA
GACCAAGAAGCACCCAATGCTTCCCAACCTACACCACCCCCACTGCCAAAGAAGATGATC
ATAAGAGCCAATACAGAGCCAATCTCCAAGGACCTCCAAAAATCCATGGAAAGTAGTCTT
TGTGTCATGGCTAATCCCACCTATGATATCGACCCCAACTGGGATGCCAGCAGTGCTGGT
TCTTCCATCAGCTATGAACTCAAAGGACTGGACATTGAGTCTTATGACTCCTTGGAAAGG
CCTTTGCGCAAGGAGAGACCTGTCCCCTCAGCAGCAAACAGCATTTCCAGCTTAACCACT
CTCAGTATTAAGGATAGATTTTCCAACAGCATGGAATCCCTCTCCAGCCGGCGTGGGCCC
TCTTGCAGACAGGGCCGAGGCATCCAGAAGCCGCAGAGACAAGCACTTTATCGAGGACTT
GAGAATCGGGAGGAAGTAGTGGGTAAAATCCGAAGCCTTCATACAGATGCCTTGAAGAAA
CTGGCTGTTAAATGCGAAGACCTTTTCATGGCTGGGCAGAAAGACCAGCTCCGTTTTGGA
GTGGACAGCTGGTCAGACTTCAGGCTAACCAGTGACAAACCATGTTGTGAGGCAGGTGAT
GCGGTTTACTATACTGCTTCATATGCAAAAGATCCACTTAATAACTATGCAGTCAAGATC
TGTAAGAGCAAAGCTAAAGAATCTCAGCAGTATTATCACAGCTTGGCTGTCCGGCAGAGT
CTGGCTGTCCATTTTAACATTCAGCAGGACTGTGGTCATTTCCTTGCTGAAGTCCCTAAC
CGTCTGCTTCCCTGGGAGGATCCAGATGACCCTGAAAAGGATGAGGATGACATGGAAGAG
ACTGAAGAAGACGCCAAAGGAGAAACGGATGGGAAAAACCCAAAGCCCTGTTCTGAAGCA
GCATCATCCCAGAAAGAGAATCAGGGAGTCATGAGCAAGAAGCAGAGGAGCCACGTTGTG
GTCATCACCAGGGAGGTTCCATGTCTTACTGTGGCTGATTTTGTGCGAGACTCTCTGGCC
CAGCATGGGAAAAGCCCTGATTTGTATGAGAGGCAGGTGTGTCTGCTGCTCTTACAGCTA
TGCTCTGGTCTTGAGCACCTCAAACCCTACCATGTCACTCACTGCGATCTACGCCTAGAG
AACCTGCTACTTGTCCACTACCAGCCTGGGGGGACTGCCCAAGGCTTTGGGCCTGCAGAG
CCCACCCCCACCTCATCTTATCCCACTAGGCTTATAGTGAGCAACTTCTCTCAGGCCAAG
CAGAAGAGCCATCTGGTGGACCCCGAGATCCTCCGGGACCAGTCTCGCCTTGCCCCAGAG
ATCATAACAGCTACCCAGTATAAAAAGTGTGATGAGTTCCAGACAGGCATCCTCATCTAT
GAGATGCTGCACCTACCCAACCCCTTTGATGAGAACCCAGAGCTGAAGGAGAGGGAATAC
ACACGAGCAGACCTGCCTCGCATCCCATTCCGCTCCCCCTACTCCCGGGGTCTGCAGCAG
CTGGCCAGCTGCCTCCTGAATCCCAACCCTTCTGAGCGGATCCTCATTTCAGACGCCAAA
GGCATCCTCCAGTGTCTGCTCTGGGGCCCCCGCGAAGATCTCTTCCAGACTTTCACCGCC
TGCCCTAGCCTAGTACAGAGGAACACCCTGCTCCAAAACTGGCTAGACATCAAGCGAACA
CTGCTCATGATCAAGTTTGCTGAGAAGTCCCTGGACAGGGAAGGTGGAATCAGCCTTGAG
GACTGGCTTTGTGCTCAGTATTTGGCTTTTGCCACTACAGACTCCCTCAGTTGTATTGTG
AAAATTCTGCAGCACCGTTTG
MSACNTFTEHVWKPGECKNCFKPKSLHQLPPDPEKAPITHGNVKTNANHSNNHRIRNTGN FRPPVAKKPTIAVKPTMIVADGQSICGELSIQEHCENKPVIIGWNRNRAALSQKPLNNNN EDDEGISHVPKPYGNNDSAKKMSDNNNGLTEVLKEIAGLDTAPQIRGNETNSRETFLGRI NDCYKRSLERKLPPSCMIGGIKETQGKHVILSGSTEVISNEGGRFCYPEFSSGEESEEDV LFSNMEEEHESWDESDEELLAMEIRMRGQPRFANFRANTLSPVRFFVDKKWNTIPLRNKS LQRICAVDYDDSYDEILNGYEENSVVSYGQGSIQSMVSSDSTSPDSSLTEESRSETASSL SQKICNGGLSPGNPGDSKDMKEIEPNYESPSSNNQDKDSSQASKSSIKVPETHKAVLALR LEEKDGKIAVQTEKEESKASTDVAGQAVTINLVPTEEQAKPYRVVNLEQPLCKPYTVVDV SAAMASEHLEGPVNSPKTKSSSSTPNSPVTSSSLTPGQISAHFQKSSAIRYQEVWTSSTS PRQKIPKVELITSGTGPNVPPRKNCHKSAPTSPTATNISSKTIPVKSPNLSEIKFNSYNN AGMPPFPIIIHDEPTYARSSKNAIKVPIVINPNAYDNLAIYKSFLGTSGELSVKEKTTSV ISHTYEEIETESKVPDNTTSKTTDCLQTKGFSNSTEHKRGSVAQKVQEFNNCLNRGQSSP QRSYSSSHSSPAKIQRATQEPVAKIEGTQESQMVGSSSTREKASTVLSQIVASIQPPQSP PETPQSGPKACSVEELYAIPPDADVAKSTPKSTPVRPKSLFTSQPSGEAEAPQTTDSPTT KVQKDPSIKPVTPSPSKLVTSPQSEPPAPFPPPRSTSSPYHAGNLLQRHFTNWTKPTSPT RSTEAESVLHSEGSRRAADAKPKRWISFKSFFRRRKTDEEDDKEKEREKGKLVGLDGTVI HMLPPPPVQRHHWFTEAKGESSEKPAIVFMYRCDPAQGQLSVDQSKARTDQAAVMEKGRA ENALLQDSEKKRSHSSPSQIPKKILSHMTHEVTEDFSPRDPRTVVGKQDGRGCTLVTTAL SLPELEREDGKEDISDPMDPNPCSATYSNLGQSRAAMIPPKQPRQPKGAVDDAIAFGGKT DQEAPNASQPTPPPLPKKMIIRANTEPISKDLQKSMESSLCVMANPTYDIDPNWDASSAG SSISYELKGLDIESYDSLERPLRKERPVPSAANSISSLTTLSIKDRFSNSMESLSSRRGP SCRQGRGIQKPQRQALYRGLENREEVVGKIRSLHTDALKKLAVKCEDLFMAGQKDQLRFG VDSWSDFRLTSDKPCCEAGDAVYYTASYAKDPLNNYAVKICKSKAKESQQYYHSLAVRQS LAVHFNIQQDCGHFLAEVPNRLLPWEDPDDPEKDEDDMEETEEDAKGETDGKNPKPCSEA ASSQKENQGVMSKKQRSHVVVITREVPCLTVADFVRDSLAQHGKSPDLYERQVCLLLLQL CSGLEHLKPYHVTHCDLRLENLLLVHYQPGGTAQGFGPAEPTPTSSYPTRLIVSNFSQAK QKSHLVDPEILRDQSRLAPEIITATQYKKCDEFQTGILIYEMLHLPNPFDENPELKEREY TRADLPRIPFRSPYSRGLQQLASCLLNPNPSERILISDAKGILQCLLWGPREDLFQTFTA CPSLVQRNTLLQNWLDIKRTLLMIKFAEKSLDREGGISLEDWLCAQYLAFATTDSLSCIV KILQHRL
Coding Sequence Details
c3224t,S1075L; g4625c,S1542T
Recommended Growth Condition: