Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH003435.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR201
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
ScCD00023490
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3495nts
Open reading frame: 1 to 3495
ATGGCTGGATTCAGCTTCGCCAAGAAATTCACTCACAAGAAACATGGAAAGACGCCTTCT
GATGCTTCTATCTCTGACCAATCTAGAGAAGCTTCACTGTCAACTCCTCCAAATGAAAAA
TTTTTTACAAAACAAGAAACGCCTCAGAAAGGGCGTCAATTTTCACAAGGTTACCATTCT
AATGTTAATAAAACTAGCTCACCACCTATGTTTGCCAGAAAACAGGTATCCGAATCAAGG
ATTCAACCGAGCGCAGTACCACCGCAACAGCGAAATGTCTCAGGACCGTCCACTACTCTC
CATAAGCAGCTTTCAAAACAGAGAGAATATACCGTATGGAATAGAATCAAGTTACAGAAT
TCTCCTTTTCCACGTTATAGACATGTTGCTTCAGCATATGTCACAGACAAGAACCAGATA
TATGTTATTGGGGGACTTCATGACCAATCTGTGTACGGCGATACATGGATATTAACGGCC
TTCGATAATGCCACCAGATTTTCCACTACAACGATAGACATAAGTGAAGCTACACCACCA
CCAAGGGTAGGCCACGCTGCTGTATTATGCGGGAATGCCTTTGTTGTATTTGGCGGTGAC
ACACACAAAGTCAATAAGGAAGGTTTGATGGACGATGACATATACCTTTTGAATATTAAT
TCCTATAAATGGACAGTCCCGGCACCCGTGGGACCACGTCCATTGGGAAGGTACGGTCAT
AAAATTTCGATTATCGCCACAACTCAAATGAAAACAAAACTGTACGTGTTTGGTGGTCAA
TTTGATGACACCTACTTCAACGACTTGGCTGTTTACGACTTATCTTCTTTTAGAAGACCG
GACTCTCATTGGGAATTTTTGAAACCAAGGACCTTTACACCACCACCTATTACCAATTTT
ACTATGATATCATACGATTCTAAACTATGGGTATTTGGTGGCGATACCTTACAAGGTTTA
GTAAATGATGTGTTTATGTACGACCCTGCAATAAATGATTGGTTTATCATTGATACCACG
GGTGAAAAGCCTCCTCCAGTTCAAGAACATGCTACTGTAGTCTACAACGATCTGATGTGT
GTTGTAGGCGGGAAAGATGAGCATGATGCTTATTTGAACTCCGTGTATTTCCTAAACTTG
AAATCGCGTAAATGGTTTAAATTACCTGTATTTACGGCTGGAATTCCGCAAGGCCGTTCT
GGGCATTCTTTAACATTATTAAAAAATGATAAAATTTTGATTATGGGAGGAGATAAATTT
GATTACGCTAGAGTCGAGGAGTACGACTTGCATACCTCTGATATTGATATGCAAAGAGGT
ACAATTGTTTATACGCTAGCCTTGGCCCGCATAAAGGACCTTTGTCCAGGCGTAATGGAT
GTTCCAACTGATACGCCAACTCCGAGAAATGGTAATCTTGACCTGGCGACGCCCGTTACC
CCAACCTCTCATCAAACTAAAAATATGAACGTACCAATTTCAGCAGCTCCTCTAGCTTCC
GCTCCATCACCTGCACCAAAAGATTTTTCAGATGCTGATCGCCTCAACAGGGAGGTACAC
AATCGAAATGTGTCAACAGAACACCAAAATCAGAGCCACCCAGTAAATTCTGAATCTCAC
TTGATTGCTGAGCCTAACATTTTGACTCCATATGTTCCATCTGAAAGCTCACAAACGCCG
GTTATGAAAATAACTTCCAACAAACCATTTGATACGCCTACTATCCAGAAAGAACCTGAT
CTTTCTGAAACTATGGATCCAACAGTTGGAAATCAGCGCATCCCATCTTCAATCTATGGA
GATAATCTCACCCCTGCAAACCAAATAAAGAATAACTCCCCAATATTAGAGACGCTTCCA
AGTAATGAGATAAAAACGCCCCAAAATGGTAATATAGAGGAAATAAAACATCTTCCTGAC
GCTGATGAAAAAATAGATTCCACGACGACCTTCGACCAAGAGATAAACGGGGACAAACTA
GGCACATCTTCGATGTCCAAGGTTGAAGAGGACGGCAATGTTGCTGACGAAGACGACGAA
ATCGGTGTGGCTCAAATGGCGAGCTCCCCATCCAAAGATCAGTTCAAAATAAAGCATTAT
AATGAATCTTCAGAATTATCACAGAATAATACGGAGATCGACAAACTGTCCGAGCCTGTG
GATATTACAATAAAGAAAAGCGACACCGCGGGCCATGATAGTGCTAACCATGTTATTGAT
GCAAGTGATGAAAAAAACGTGTCCCCAATGGGAGACGTTCCAACTGATACGAAAAATGAA
GAAGCTAGCGTTCCTATAAATAGAGATGCTACTACCGAAGTCGTAGATAGGGCACTATTT
GAGAAATTAAGATCGGAATTGCAAAGTTTGAAAGAATTGACGCACGAAAAGGCGCTTGAA
GCCGGAGCTCACATAAAGGAATTAGAGACGGAGCTATGGCAGTTAAAATCCCAGAAGAAT
TCTGGGACAACGAAAGAAATAGATGAATTGGATTCCGTCAGACTGCAATCAAAATGTGAA
ATACTAGAGGCAGATAATCATTCCTTGGAAGATAAAGTTAATGAGCTAGAAGAGTTGGTG
AATAGCAAATTCTTAGACATAGAGAATCTAAATGAAGTTATACAATTTCAGAACGAAAAA
ATTAAATCCTTAGAACTAGAGCCTAATTACAAAGAAAAACTTGAAGAACTACAGATAGAA
CATGAGAATTTGAGTAGAGAAAATGAAAGGTTGAAAAATGAAAGCAAACAACATAACGAA
GATATAATCAATAATGTTGCGAATTACTCGTCACAGTTAGGCTCATTGATCAGTCATTGG
AAAGAAAACAGAGCCAACTCTTCGTTTCTTGAATCTTCTTCTTCTTTGATATCTGTATCG
GATGAAAATGGCGAGAAAACTGTCGGTGAACCCTATGGTGATCAAAGTCGTCACCACCGT
GTAGTGATTAACAAACTAACGAATAGATTGGACGATTTGCTGGAGAGAAGTCAAGAGCTA
ACAATATCTAAAGAAAAACTATCTTCTGAATACCATGCTTTAAAGATGGAACACAGCTCA
TTAAGTCAAGATGTATTAGTAAAAGAGAATGAAATCAAAAAAATACAGAATGATTACAAA
GAAAGTATCAGTTCTATGGACAGTGCAAGTAAGGCACTAATGGTTTCACAAAGAGAGCTA
GAGAAGTACAAGAGTTTGAATAAAAAGTTAATCGACGAGTTAGATGAATTGAAGTTCAAA
AATGGCGTCTGCAGTGAAAATTTCGAAAATGGTCTCAGAAGCACCGAGGAGAGTTCCAAT
AATGTAAAAAATAGCAACAGCATAAGAGAAAATCAATTCAACATCAAAATAAATGATCTG
AAAGCGGAACTCTTCATCACTAATCAAGAAAGAGATGACTTGAAAAGTGAAGTGTTAGAG
TTGAAAAAGAGATTATTGAATTTGGAAAATAACACCAAACAAGTTAATGAAGATGCTGAC
AGCGATCTACTATAA
Protein sequence predicted based on nucleotide sequence
MAGFSFAKKFTHKKHGKTPSDASISDQSREASLSTPPNEKFFTKQETPQKGRQFSQGYHS NVNKTSSPPMFARKQVSESRIQPSAVPPQQRNVSGPSTTLHKQLSKQREYTVWNRIKLQN SPFPRYRHVASAYVTDKNQIYVIGGLHDQSVYGDTWILTAFDNATRFSTTTIDISEATPP PRVGHAAVLCGNAFVVFGGDTHKVNKEGLMDDDIYLLNINSYKWTVPAPVGPRPLGRYGH KISIIATTQMKTKLYVFGGQFDDTYFNDLAVYDLSSFRRPDSHWEFLKPRTFTPPPITNF TMISYDSKLWVFGGDTLQGLVNDVFMYDPAINDWFIIDTTGEKPPPVQEHATVVYNDLMC VVGGKDEHDAYLNSVYFLNLKSRKWFKLPVFTAGIPQGRSGHSLTLLKNDKILIMGGDKF DYARVEEYDLHTSDIDMQRGTIVYTLALARIKDLCPGVMDVPTDTPTPRNGNLDLATPVT PTSHQTKNMNVPISAAPLASAPSPAPKDFSDADRLNREVHNRNVSTEHQNQSHPVNSESH LIAEPNILTPYVPSESSQTPVMKITSNKPFDTPTIQKEPDLSETMDPTVGNQRIPSSIYG DNLTPANQIKNNSPILETLPSNEIKTPQNGNIEEIKHLPDADEKIDSTTTFDQEINGDKL GTSSMSKVEEDGNVADEDDEIGVAQMASSPSKDQFKIKHYNESSELSQNNTEIDKLSEPV DITIKKSDTAGHDSANHVIDASDEKNVSPMGDVPTDTKNEEASVPINRDATTEVVDRALF EKLRSELQSLKELTHEKALEAGAHIKELETELWQLKSQKNSGTTKEIDELDSVRLQSKCE ILEADNHSLEDKVNELEELVNSKFLDIENLNEVIQFQNEKIKSLELEPNYKEKLEELQIE HENLSRENERLKNESKQHNEDIINNVANYSSQLGSLISHWKENRANSSFLESSSSLISVS DENGEKTVGEPYGDQSRHHRVVINKLTNRLDDLLERSQELTISKEKLSSEYHALKMEHSS LSQDVLVKENEIKKIQNDYKESISSMDSASKALMVSQRELEKYKSLNKKLIDELDELKFK NGVCSENFENGLRSTEESSNNVKNSNSIRENQFNIKINDLKAELFITNQERDDLKSEVLE LKKRLLNLENNTKQVNEDADSDLL*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
3495
Mutations:
None
Discrepancy:
c126t; t198c;
t402c; a1340c,D447A
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR201
Synonyms:
''
Sequencing Primer:
Forward:
pDONR201-forward
Reverse:
pDONR201-reverse
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with attL1 and attL2 and rrnB T2 and rrnB T1 and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4470
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
959
1264
CDS
CmR
confers resistance to chloramphenicol
1606
2265
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
2868
3677
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
73
100
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
192
278
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2266
2368
protein binding site
attP1
recombination site for the Gateway BP reaction
332
563
protein binding site
attP2
recombination site for the Gateway BP reaction
2513
2744
recombination site
attL1
attL recombination site 1 present in recombined (with insert) form only, position is approximate
332
563
recombination site
attL2
attL recombination site 2 present in recombined (with insert) form only, position is approximate
2744
2513
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
3820
4408
