Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH148674.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
ScCD00024283
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4968nts
Open reading frame: 1 to 4968
ATGGCTACACCTTCATTTGGCAATTCCTCTCCACAATTGACCTTCACTCATGTTGCTAAT
TTTATGAACGATGCTGCTGCTGATGTATCCGCTGTGGATGCCAAGCAATTGGCCCAAATA
AGACAGTTTTTGAAAGCTAACAAGACAAATCTTATCGAGAGTCTGAACACAATAAGGCAG
AATGTAACTTCTTCCGGCGATCATAACAAGTTACGCTCTACAATTGCAAACTTATTGCAA
ATAAACGTCGATAATGATCCATTCTTTGCTCAATCTGAGGACCTTTCTCATGCTGTAGAA
TTCTTTATGTCTGAGAGATCATCTAGACTTCATATAGTATACTCACTTCTCGTTAACCCT
GACATCGACCTCGAGACATATTCATTTATTGACAACGATAGATTCAATGTAGTGGGGAAA
TTGATTTCTATAATATCATCTGTAATCCAGAACTACGACATTATTACAGCTTCATCTTTA
GCACATGATTATAATAACGATCAAGACATGTTCACAATTGTCTCATTGGTGCAGTTAAAA
AAATTTTCTGATTTGAAGTTTATCTTGCAGATATTACAAATTTTGAATTTAATGATCCTC
AACACAAAAGTACCAGTAGATATCGTCAATCAATGGTTTCTACAGTATCAAAATCAATTT
GTTGAATTTTGCAGAAATATAAATTCAACAGACAAGAGTATAGATACCTCGTCACTACAA
TTGTACAAGTTTCAGAATTTCCAAGACCTCAGTTACCTCTCAGAAACGCTTATTTCCAGG
ATTTCATCTTTATTTACTATCACAACTATATTGATACTTGGACTGAACACTTCAATCGCG
CAATTTGATATTCAGTCACCTTTGTACATGGATACAGAAACTTTCGACACCGTCAATAGC
GCTCTAGAGAATGATGTAGCAACCAATATCGTCAACGAGGACCCCATTTTCCATCCAATG
ATTCATTACTCCTGGTCATTTATACTTTATTATAGGCGAGCATTACAATCGTCGGAATCA
TTTGATGATTCAGACATAACGAAATTTGCTCTGTTTGCTGAATCACATGATGTTCTTCAA
AAATTGAATACTCTCTCAGAAATTCTATCCTTTGATCCTGTTTACACCACAGTAATAACA
GTATTTTTGGAGTTTTCTTTAAATTTTATTCCTATAACTGCATCTACTTCCCGGGTTTTC
GCCAAGATAATTTCAAAGGCACCAGAGCAGTTCATTGAGAATTTCTTAACTAATGACACT
TTTGAGAAAAAATTGAGCATCATAAAGGCTAAATTGCCCCTATTGAACGAATCACTGATC
CCGCTAATTAATTTAGCCTTAATTGATACCGAGTTTGCCAATTTTGAATTAAAAGATATA
TGTTCATTTGCTGTTACCAAAAGTAGTTTGAACGATTTGGATTATGACTTAATTGCTGAT
ACAATAACAAACTCTTCTTCATCCTCCGATATTATAGTGCCTGATTTGATAGAGTTGAAG
TCTGATTTACTAGTGGCGCCACCCTTAGAAAACGAAAACTCTAATTGTCTCTTGTCAATA
CCAAAATCAACAAAGGGAAAAATTCTTACTATCAAACAACAACAACAACAACAACAACAA
CAGAATGGACAACAGCCGCCAACTACTTCCAATTTGATTATTTTCCTTTACAAATTCAAT
GGTTGGTCTTTGGTGGGTAGAATATTGCAAAATCTATTACATTCATATATGGAAAAGGGT
ACACAACTGGATGACTTACAACATGAATTGATGATATCCATAATAAAGTTAGTCACTAAC
GTTGTCGATCCGAAAACCTCGATCGAGAAATCTAGTGAAATTCTGTCATATTTATCAAAT
TCTCTGGATACTTCAGCCAGCACCATAAATGGAGCGTCGATTATCCAAGTAATTTTCGAG
ATTTTTGAAATATCACTACAAAGAAAAGATTATACCTCAATTGTACAATGTTGTGAATTT
ATGACTATGCTGACACCGAATTACCTTCATCTTGTTTCTTCTTACTTGAACAAATCGGAT
TTGTTAGACAAATATGGTAAGACTGGTCTTTCCAATATGATACTCGGTTCGGTTGAATTA
TCTACGGGGGATTACACATTCACTATCCAATTGCTTAAGCTAACAAAAGTCTTTATTCGA
GAATCTCTCTCATTGAAAAATATCCATATTTCCAAGAGAAGTAAAATTGATATTATTAAT
AAGTTGATTCTACATGCAATTCATATCTTTGAGAGTTATTACAACTGGAAGTACAACAAC
TTCTTACAAAAATTCGAAATTGCCTTCCATTTGACATTAATTTTTTATGATGTACTTCAT
GACGTTTTCACCATAAATCCACATCAAAAGGATCAGTTAATCATTTCCTCGTCAGCAAAC
AAATTGTTGCAGTTATTTTTGACTCCCATGGATTCCATTGATTTGGCTCCAAATACTTTG
ACGAATATTCTGATTTCACCATTGAATACCACAACAAAAATTTTAGGCGACAAAATTTTG
GGTAATTTATATAGCAAGGTAATGAATAACTCCTTCAAGCTGTGCACGCTTTTGATCGCT
ATTCGAGGTAGCAATCGTGATTTAAAGCCAAGCAACCTGGAAAAATTGCTTTTCATAAAT
TCATCAAAATTGGTTGACGTTTATACCCTACCAAGTTATGTTCATTTCAAAGTGCAGATC
ATAGAGTTGTTAAGCTATTTAGTAGAGGCTCCTTGGAATGACGACTATCCATTTCTGTTA
TCTTTCCTTGGTGAAGCAAAGTCAATGGCATTTTTGAAAGAAGTCCTGTCGGACCTGAGC
TCTCCAGTGCAAGATTGGAACCTTCTCCGAAGTTTGTACATTTTTTTTACTACCCTATTA
GAAAGTAAACAAGATGGGTTATCTATCCTCTTCTTAACAGGTCAATTTGCCTCCAATAAA
AAAATTAATGACGAATCCTCCATAGACAAAAAGAGTTCTATATTAACTGTTTTGCAAAAG
AATTCTTTACTATTAGATTCAACGCCTGAAGAAGTAAGTTGCAAGTTATTAGAAACGATA
ACATATGTTTTAAACACTTGGACAAATTCTAAAATATTCATAAAAGATCCTAAATTTGTG
AATTCCTTATTGGCTAAGTTGAAGGACTCAAAAAAATTATTTCAGAAAAAGGAAAATTTG
ACTCGTGACGAAACTGTTTCTTTGATTAAGAAATACAAATTGATCTCAAGGATTGTAGAA
ATATTTGCGTTGTGTATTTACAACTCCACTGATTCAAACTCGGAAATTTTAAATTTTTTG
AATCAGGAAGACTTGTTTGAATTAGTTCATCATTTCTTCCAAATTGATGGGTTCAATAAA
ACTTTTCATGATGAATTAAACTTAAAATTTAAAGAGAAATGGCCCAGTTTAGAGCTTCAG
TCGTTCCAAAAAATTCCATTATCCAGAATAAACGAAAATGAGAATTTCGGTTATGATATT
CCGTTATTAGATATAGTTTTAAAAGCTGACCGTAGTTGGAACGAACCAAGTAAGAGTCAG
ACAAATTTTAAGGAGGAAATAACAGACGCTTCATTAAATTTGCAGTATGTCAATTACGAA
ATATCTACCGCAAAAGCATGGGGCGCACTAATCACCACGTTTGTGAAGAGGAGCACTGTT
CCGTTAAATGATGGTTTTGTCGACTTGGTAGAGCATTTCCTCAAGTTAAATATTGATTTT
GGTTCAGATAAACAAATGTTTACCCAAATATATCTGGAAAGAATTGAGTTGTCGTTTTAC
ATTTTATATTCGTTCAAGTTGTCGGGAAAATTGTTGAAGGAAGAAAAGATAATTGAATTG
ATGAACAAGATTTTTACAATTTTCAAATCTGGTGAGATTGACTTTATTAAAAATATTGGT
AAGTCACTAAAAAATAACTTTTATAGACCTCTTTTGAGATCTGTCTTAGTTCTTCTCGAG
TTAGTCTCGTCAGGAGATCGTTTTATCGAATTAATATCTGATCAACTACTGGAATTTTTT
GAATTAGTGTTTAGTAAGGGTGTTTATTTGATACTGTCTGAGATATTATGTCAGATCAAT
AAATGCTCCACGAGGGGTTTAAGCACCGATCACACCACCCAAATTGTCAACTTGGAGGAT
AATACTCAGGATTTATTGTTATTATTATCACTCTTCAAGAAGATCACCAATGTGAATCCC
TCTAAAAACTTCAATGTTATTCTAGCATCATCCCTAAACGAAGTAGGAACTTTGAAAGTT
ATACTGAACCTATATTCAAGCGCGCATTTGATAAGGATTAACGATGAACCAATTCTTGGG
CAAATTACTCTGACCTTTATCTCCGAATTGTGTTCTATTGAGCCAATTGCTGCAAAACTC
ATCAACAGTGGATTGTATAGCGTTCTATTGGAAAGTCCACTATCTGTTGCAATACAGCAA
GGTGATATAAAACCTGAATTTTCGCCTAGATTGCACAACATTTGGAGCAATGGTTTGTTA
TCCATTGTTCTACTACTATTAAGTCAGTTCGGTATCAAGGTTTTACCTGAGACGTGCCTA
TTTGTTTCATATTTTGGAAAACAAATAAAATCTACAATATATAACTGGGGTGACAACAAG
TTAGCAGTGTCAAGTTCGTTGATAAAGGAAACGAATCAATTGGTTTTATTGCAAAAAATG
CTAAACTTGCTAAATTACCAAGAATTGTTTATTCAGCCTAAAAACTCTGATGATCAACAA
GAAGCTGTAGAATTGGTTATTGGTCTAGATTCTGAACATGATAAAAAAAGGTTAAGCGCT
GCATTAAGCAAGTTTCTAACACATCCAAAATACTTGAATTCAAGAATAATACCTACAACT
TTAGAAGAGCAACAGCAGTTGGAGGATGAATCAAGCAGATTGGAGTTCGTCAAGGGTATC
AGCAGAGACATTAAAGCATTACAAGATTCACTATTTAAGGACGTTTAG
Protein sequence predicted based on nucleotide sequence
MATPSFGNSSPQLTFTHVANFMNDAAADVSAVDAKQLAQIRQFLKANKTNLIESLNTIRQ NVTSSGDHNKLRSTIANLLQINVDNDPFFAQSEDLSHAVEFFMSERSSRLHIVYSLLVNP DIDLETYSFIDNDRFNVVGKLISIISSVIQNYDIITASSLAHDYNNDQDMFTIVSLVQLK KFSDLKFILQILQILNLMILNTKVPVDIVNQWFLQYQNQFVEFCRNINSTDKSIDTSSLQ LYKFQNFQDLSYLSETLISRISSLFTITTILILGLNTSIAQFDIQSPLYMDTETFDTVNS ALENDVATNIVNEDPIFHPMIHYSWSFILYYRRALQSSESFDDSDITKFALFAESHDVLQ KLNTLSEILSFDPVYTTVITVFLEFSLNFIPITASTSRVFAKIISKAPEQFIENFLTNDT FEKKLSIIKAKLPLLNESLIPLINLALIDTEFANFELKDICSFAVTKSSLNDLDYDLIAD TITNSSSSSDIIVPDLIELKSDLLVAPPLENENSNCLLSIPKSTKGKILTIKQQQQQQQQ QNGQQPPTTSNLIIFLYKFNGWSLVGRILQNLLHSYMEKGTQLDDLQHELMISIIKLVTN VVDPKTSIEKSSEILSYLSNSLDTSASTINGASIIQVIFEIFEISLQRKDYTSIVQCCEF MTMLTPNYLHLVSSYLNKSDLLDKYGKTGLSNMILGSVELSTGDYTFTIQLLKLTKVFIR ESLSLKNIHISKRSKIDIINKLILHAIHIFESYYNWKYNNFLQKFEIAFHLTLIFYDVLH DVFTINPHQKDQLIISSSANKLLQLFLTPMDSIDLAPNTLTNILISPLNTTTKILGDKIL GNLYSKVMNNSFKLCTLLIAIRGSNRDLKPSNLEKLLFINSSKLVDVYTLPSYVHFKVQI IELLSYLVEAPWNDDYPFLLSFLGEAKSMAFLKEVLSDLSSPVQDWNLLRSLYIFFTTLL ESKQDGLSILFLTGQFASNKKINDESSIDKKSSILTVLQKNSLLLDSTPEEVSCKLLETI TYVLNTWTNSKIFIKDPKFVNSLLAKLKDSKKLFQKKENLTRDETVSLIKKYKLISRIVE IFALCIYNSTDSNSEILNFLNQEDLFELVHHFFQIDGFNKTFHDELNLKFKEKWPSLELQ SFQKIPLSRINENENFGYDIPLLDIVLKADRSWNEPSKSQTNFKEEITDASLNLQYVNYE ISTAKAWGALITTFVKRSTVPLNDGFVDLVEHFLKLNIDFGSDKQMFTQIYLERIELSFY ILYSFKLSGKLLKEEKIIELMNKIFTIFKSGEIDFIKNIGKSLKNNFYRPLLRSVLVLLE LVSSGDRFIELISDQLLEFFELVFSKGVYLILSEILCQINKCSTRGLSTDHTTQIVNLED NTQDLLLLLSLFKKITNVNPSKNFNVILASSLNEVGTLKVILNLYSSAHLIRINDEPILG QITLTFISELCSIEPIAAKLINSGLYSVLLESPLSVAIQQGDIKPEFSPRLHNIWSNGLL SIVLLLLSQFGIKVLPETCLFVSYFGKQIKSTIYNWGDNKLAVSSSLIKETNQLVLLQKM LNLLNYQELFIQPKNSDDQQEAVELVIGLDSEHDKKRLSAALSKFLTHPKYLNSRIIPTT LEEQQQLEDESSRLEFVKGISRDIKALQDSLFKDV*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
4968
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
