Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH149139.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
ScCD00024302
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4419nts
Open reading frame: 1 to 4419
ATGGATATTCCCTCTTCAAACCAAATTCAACATGGCCAAAGGAGTGAAAGGAACCGAAGA
ATGCCGAGGGCTTCTTTTTCTTCTACAGCCACTACTTCTACGGCGGCCACATTGACTTCC
GCGATGGTACTGGATCAGAACAATTCGGAACCGTACGCAGGTGCAACATTCGAAGCCGTG
CCAAGTTCTATTGTATCATTCCATCACCCTCATTCTTTTCAATCAAGCAACCTCCCAAGT
CCGCATTCTTCAGGGAACCTAGAGCAGAGAGGCCGCCGATTGACTGAATCAGAGCCTTTA
GTATTATCCTCGGCTGAACAGTCTCGAAGCTCTTCTAGAAACCCTTCTCATTTTCGATTT
TTCACTCAGGAGCAAATATCCAATGCTGAAGGCGCTTCCACGTTAGAAAATACAGATTAT
GACATGGCCTGGGATGCCACCCCCGCTTATGAACAAGATAGAATCTATGGTACGGGCCTA
TCTTCCAGGCGTTCTTCTATTCGAAGTTTTTCCAGAGCATCATCTCTCTCAAATGCAAAG
TCTTATGGATCTTTCAGTAAGAGGGGTCGCTCTGGTTCAAGGGCACCACAAAGGCTGGGA
GAAAATTCTGACACTGGGTTCGTTTATCATTCCGCTACACATTCATCATCCAGTTTATCG
AGATATACGACCAGAGAAAGAATTCCTATTGAACTAGAAAGTCAAACAGATGAAATACTA
GAAGACGAGTCTTCTACACATTCTTTAGAATCTTCAGATAGTAGGCGATCTGCAAGCGAA
AATAATAGAGGAAGCTTTTCTGGTCATGATGATGTTCATAATCAGCATAGCGAATATCTA
AAACCTGACTATCATGAAAAGTTTTATCCACAGTATGCACCAAACTTGCATTACCAAAGG
TTTTACATTGCGGAAGAGGATTTAGTTATTGGTATCGCTGCTTACCAGACCTCAAAATTC
TGGTATATTATTTACAACCTGTGCTGCTTCTTGACTTTCGGATTAGTTTATCTTTTAACG
AGATGGCTTCCCCATCTCAAAGTTAAATTATATGGCGTGAAAGTGCCATTGGCTAAAGCA
GAATGGGTCGTGATAGAAAATGAGTTTGGTGAGTTTGTCATACAACCTATCGATCGACAG
TGGTATAATAGACCATTAAGTACTGTTTTACCTTTTGAAAATTACCCTAATCCGTCATAT
GAACCAAATGATATCAATTTGAGCCACCACCATGCCAATGAAATTAATCCAAATGTGCCT
ATCTTGATCACATTTGAATACAGATATATTAAGTTCATATATTCACCGCTCGATGATCTC
TTTAAAACTAACAATAACTGGATAGATCCTGACTGGGTGGATTTAAGTACCGTATCAAAT
GGTTTAACTAAGGGGGTTCAAGAAGATAGAGAACTTGCGTTTGGTAAAAATCAAATTAAT
TTGAGAATGAAAACTACTTCAGAAATTTTATTCAATGAAGTGTTGCATCCGTTTTATGTC
TTTCAAGTATTTTCGATAATTTTGTGGGGCATTGATGAATACTACTACTACGCCGCATGC
ATTTTTTTGATTTCTGTACTGTCTATTTTTGATTCATTGAATGAACAGAAGAAAGTATCG
AGAAACTTAGCTGAAATGTCGCACTTTCATTGTGACGTTCGTGTTTTGAGGGATAAGTTT
TGGACAACGATAAGCTCATCAGAACTAGTTCCGGGCGATATATATGAAGTATCTGACCCA
AATATAACAATATTGCCTTGTGACTCTATTCTTCTGTCCAGTGATTGTATAGTCAATGAA
TCCATGCTAACTGGTGAATCTGTGCCCGTTTCGAAATTTCCAGCTACGGAAGAAACAATG
TACCAATTATGTGACGACTTCCAGAGCACCCAAATATCGAGTTTTGTTTCCAAATCCTTC
TTGTATAATGGTACAAATATAATTAGAGCAAGAATTGCCCCCGGGCAAACCGCCGCTTTG
GCGATGGTTGTAAGGACAGGATTCTCAACAACAAAGGGCTCCTTGGTTCGTTCCATGGTT
TTCCCAAAACCAACTGGTTTTAAATTCTATAGAGATTCCTTTAAATATATTGGATTCATG
TCATTAATCGCAATTTTCGGTTTTTGCGTCAGTTGCGTTCAGTTCATTAAACTTGGTTTA
GACAAAAAGACAATGATATTAAGAGCTTTAGATATCATAACAATTGTAGTGCCACCAGCT
CTGCCCGCGACTTTAACCATTGGTACCAATTTTGCTTTGAGTAGGTTAAAGGAGAAGGGA
ATATTTTGTATCTCTCCAACAAGGCTTAACATTAGTGGTAAAATCGATGTTATGTGTTTT
GATAAAACTGGAACACTTACCGAAGATGGCTTAGATGTACTTGGAGTACAAATATCGGAG
CCAAATGGGGTTAGAGGCCAGAAGTTTGGAGAACTATTGAGTGATATTCGCCAAGTATTT
CCTAAATTTTCTTTGAACGATTGTAGTTCGCCATTAGATTTTAAGTCCAGGAACTTTTTC
ATGTCATTATTAACATGTCACTCGCTGAGATCAGTTGATGGAAATTTACTCGGTGACCCA
CTAGACTTTAAAATGTTTCAATTTACCGGTTGGTCATTCGAGGAGGACTTCCAAAAACGA
GCATTCCATTCATTATACGAGGGAAGACATGAAGATGATGTTTTCCCAGAAAATTCAGAA
ATCATTCCTGCGGTAGTTCACCCTGATAGTAATAATAGAGAAAATACGTTTACTGATAAT
GATCCTCATAATTTTCTAGGGGTGGTGAGAAGCTTTGAATTTTTATCTGAGCTGAGACGT
ATGAGCGTAATTGTTAAAACGAATAATGACGACGTATATTGGTCGTTTACTAAAGGTGCG
CCCGAAGTTATTTCAGAAATATGTAATAAATCAACTTTACCTGCAGATTTTGAAGAAGTA
CTACGTTGTTACACTCATAACGGATATAGGGTTATTGCATGTGCTGGGAAGACACTTCCA
AAAAGAACTTGGTTATATTCCCAAAAAGTATCTAGGGAAGAGGTTGAGTCAAATTTGGAA
TTTTTAGGTTTCATTATCTTCCAAAATAAACTAAAAAAAGAGACCTCTGAAACTCTGAAA
AGTTTACAAGATGCAAACATCAGGACGATAATGTGTACGGGTGATAACATACTTACTGCT
ATTTCCGTAGGTAGGGAAGCTGGTTTGATACAATGCTCTCGCGTTTATGTTCCATCCATT
AATGATACACCCTTGCACGGTGAGCCCGTAATAGTATGGAGGGACGTCAATGAGCCAGAT
AAAATTTTGGATACCAAGACTTTGAAACCTGTCAAGCTGGGGAATAATAGTGTTGAAAGT
CTACGTGAGTGTAACTATACTCTTGCAGTTAGCGGCGATGTATTTAGACTGTTGTTCAGA
GATGAAAACGAGATACCAGAGGAATACTTAAACGAGATTTTATTGAATTCATCAATCTAC
GCAAGAATGTCGCCTGATGAAAAGCATGAATTGATGATACAGCTGCAGAAGCTAGATTAT
ACAGTTGGGTTCTGTGGTGATGGTGCAAATGATTGTGGGGCACTCAAAGCGGCAGACGTA
GGTATTTCCCTTTCGGAGGCTGAAGCTTCAGTTGCTGCTCCGTTCACTTCAAAGATATTT
AATATTAGCTGCGTACTTGATGTCATTAGGGAAGGGCGTGCTGCGTTAGTTACATCTTTT
GCATGTTTCCAGTACATGAGTTTGTACTCTGCCATCCAATTCATCACTATTACTATACTT
TACAGCCGTGGTTCTAATTTGGGAGATTTCCAATTCCTGTACATTGATTTATTGCTGATC
GTTCCGATCGCAATTTGCATGTCATGGTCCAAATCCTATGAAAAGATAGATAAAAAGAGG
CCTTCCGCTAACCTAGTTTCACCTAAGATTTTGGTTCCTCTTTTGATTAGCGTATTCTTG
GTGTTTTTATTCCAGTTCATTCCATGGATAATCGTACAAAAGATGAGCTGGTACATCAAG
CCAATCGTTGGGGGCGACGACGCCGTGCAATCTTCAGATAATACCGTATTGTTCTTCGTC
TCAAATTTTCAATATATTTTGACTGCTATAGTTTTGTCTGTGGGTCCACCATATAGAGAG
CCGATGTCAAAAAACTTTGAATTTATCGTTGATATCACTGTCTCTATCGGTGCAAGTCTA
CTGCTAATGACTTTGGATACAGAATCATACCTTGGTAAGATGTTACAGCTGACCCCTATA
TCAAACAGTTTTACAATGTTTATCATTGTATGGGTGATATTAAATTACTATGCTCAACTG
TATATACCGCCATCGATAAAAGGTTGGTTAAAGAAAAAGAAGAGTAGCAAAAAGTATAAA
CTACTGATACAAGAAGAAATGAAATTAAAAGAAGTTTAG
Protein sequence predicted based on nucleotide sequence
MDIPSSNQIQHGQRSERNRRMPRASFSSTATTSTAATLTSAMVLDQNNSEPYAGATFEAV PSSIVSFHHPHSFQSSNLPSPHSSGNLEQRGRRLTESEPLVLSSAEQSRSSSRNPSHFRF FTQEQISNAEGASTLENTDYDMAWDATPAYEQDRIYGTGLSSRRSSIRSFSRASSLSNAK SYGSFSKRGRSGSRAPQRLGENSDTGFVYHSATHSSSSLSRYTTRERIPIELESQTDEIL EDESSTHSLESSDSRRSASENNRGSFSGHDDVHNQHSEYLKPDYHEKFYPQYAPNLHYQR FYIAEEDLVIGIAAYQTSKFWYIIYNLCCFLTFGLVYLLTRWLPHLKVKLYGVKVPLAKA EWVVIENEFGEFVIQPIDRQWYNRPLSTVLPFENYPNPSYEPNDINLSHHHANEINPNVP ILITFEYRYIKFIYSPLDDLFKTNNNWIDPDWVDLSTVSNGLTKGVQEDRELAFGKNQIN LRMKTTSEILFNEVLHPFYVFQVFSIILWGIDEYYYYAACIFLISVLSIFDSLNEQKKVS RNLAEMSHFHCDVRVLRDKFWTTISSSELVPGDIYEVSDPNITILPCDSILLSSDCIVNE SMLTGESVPVSKFPATEETMYQLCDDFQSTQISSFVSKSFLYNGTNIIRARIAPGQTAAL AMVVRTGFSTTKGSLVRSMVFPKPTGFKFYRDSFKYIGFMSLIAIFGFCVSCVQFIKLGL DKKTMILRALDIITIVVPPALPATLTIGTNFALSRLKEKGIFCISPTRLNISGKIDVMCF DKTGTLTEDGLDVLGVQISEPNGVRGQKFGELLSDIRQVFPKFSLNDCSSPLDFKSRNFF MSLLTCHSLRSVDGNLLGDPLDFKMFQFTGWSFEEDFQKRAFHSLYEGRHEDDVFPENSE IIPAVVHPDSNNRENTFTDNDPHNFLGVVRSFEFLSELRRMSVIVKTNNDDVYWSFTKGA PEVISEICNKSTLPADFEEVLRCYTHNGYRVIACAGKTLPKRTWLYSQKVSREEVESNLE FLGFIIFQNKLKKETSETLKSLQDANIRTIMCTGDNILTAISVGREAGLIQCSRVYVPSI NDTPLHGEPVIVWRDVNEPDKILDTKTLKPVKLGNNSVESLRECNYTLAVSGDVFRLLFR DENEIPEEYLNEILLNSSIYARMSPDEKHELMIQLQKLDYTVGFCGDGANDCGALKAADV GISLSEAEASVAAPFTSKIFNISCVLDVIREGRAALVTSFACFQYMSLYSAIQFITITIL YSRGSNLGDFQFLYIDLLLIVPIAICMSWSKSYEKIDKKRPSANLVSPKILVPLLISVFL VFLFQFIPWIIVQKMSWYIKPIVGGDDAVQSSDNTVLFFVSNFQYILTAIVLSVGPPYRE PMSKNFEFIVDITVSIGASLLLMTLDTESYLGKMLQLTPISNSFTMFIIVWVILNYYAQL YIPPSIKGWLKKKKSSKKYKLLIQEEMKLKEV*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
4419
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
